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InvisibleLeft Nut City
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WBS jars question..
    #5441903 - 03/25/06 06:38 PM (17 years, 10 months ago)

I've got two 1/2 gallon jars of WBS with spores I inoculated over 2 weeks ago with absolutely no signs of life. If I get 2 different syringes and just inject these same jars would that be cool?

Or, should I just scrap this attempt and start over with fres WBS?

Thanks!!


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Re: WBS jars question.. [Re: Left Nut City]
    #5441914 - 03/25/06 06:42 PM (17 years, 10 months ago)

I would test the syringes with a smaller jar before possibly wasting them on 1/2 gallon jars again. It could be bad spores or that the grains are too dry.


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Re: WBS jars question.. [Re: Left Nut City]
    #5441920 - 03/25/06 06:44 PM (17 years, 10 months ago)

Don't scrap, big jars can take a little longer....

If the syringes are from a vendor then there probably good...

And if nothing does happen don't re-inject, just redo it

Don't combine, more then one strain in one jar...


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Re: WBS jars question.. [Re: Left Nut City]
    #5441921 - 03/25/06 06:44 PM (17 years, 10 months ago)

Also, make sure the jars are COMPLETELY cooled down before inoculating, especially with larger jars.


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Re: WBS jars question.. [Re: FooMan]
    #5441929 - 03/25/06 06:46 PM (17 years, 10 months ago)

If it is because the grains are too dry, would it help just to add some water that was boiled and then cooled to room temp?


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Re: WBS jars question.. [Re: Left Nut City]
    #5441931 - 03/25/06 06:48 PM (17 years, 10 months ago)

Yes but be sterile during the process, and you could add to much


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Re: WBS jars question.. [Re: Left Nut City]
    #5441933 - 03/25/06 06:49 PM (17 years, 10 months ago)

I wouldn't, but you could give it a shot. It's better to have the grains at optimum moisture content ahead of time. The grains may not soak up the water like you think they will.


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Re: WBS jars question.. [Re: FooMan]
    #5441945 - 03/25/06 06:54 PM (17 years, 10 months ago)

Thanks for all your replies.

My last try was TOO wet so this time when I strained the WBS before PC'ing I let it sit in a colander for over an hour and a half When I felt them they were still wet, and shaking them in the jars I can tell they have some water content in them.

I suspect the spores were duds, but after 2 weeks of no fluffy whiteness I guess injecting 10-15 cc's of sterile water won't kill.

Hell, they aren't doing anything anyways so I guess it is worth a last ditch effort.


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Re: WBS jars question.. [Re: Left Nut City]
    #5441952 - 03/25/06 06:57 PM (17 years, 10 months ago)

I did it once for a few jars that I messed up.

And it did seem to work...

Though I have no idea of how much to add.

I since make sure that it is correct......


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