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Offlineredshadow
Yaddia Holla?
I'm a teapot User Gallery

Registered: 09/28/04
Posts: 282
Loc: Zeta Reticuli
Last seen: 2 years, 9 months
contamination problems!!!!
    #5438411 - 03/24/06 01:45 PM (17 years, 10 months ago)

huhhhh, contamination again! im not sure what else to do at this point. ive pressure cooked the jars for an hour, i sterlized all the equpmetnt i used. the water i used in the substrate was filtered water and i boiled it before mixing. i also used tyvek and polyfil on the jars and taped the holes shut and the jars are wrapped in foil. what more can i do!? i still got contams in my jars, one of the cakes is almost completly colonized and is just got some contamination at he top, a coule of thers were a couple days into the colonizing and they got contaminated to. i used to boil the BRF cakes but always had lost of contams, i fuqured the pressure cooker woul get rid of all of it.help!!!


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=<{:  Its only when you've lost everything that your free to do anything.......  :}>=


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Offlinefireworks_godS
Sexy.Butt.McDanger
Male

Registered: 03/12/02
Posts: 24,855
Loc: Pandurn
Last seen: 1 year, 12 days
Re: contamination problems!!!! [Re: redshadow]
    #5438417 - 03/24/06 01:46 PM (17 years, 10 months ago)

Did you wipe down the syringe needles with alcohol, or flame-sterilize them?

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Peace. :mushroom2:


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If I should die this very moment
I wouldn't fear
For I've never known completeness
Like being here
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Loving every breath of you

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Offlineskeletor
the dude
Registered: 09/06/05
Posts: 1,235
Loc: heaven on earth
Last seen: 17 years, 4 months
Re: contamination problems!!!! [Re: fireworks_god]
    #5438508 - 03/24/06 02:07 PM (17 years, 10 months ago)

put some silicone over your inoculation holes. wipe everything with alcohole soaked cotton balls and inject through a wrapped soaked ball, through the silicon. other then that maybe its what you are injecting that is already contaminated, unless its a soponcers syringe... but if it is your own LC or a syringe you made from a print that could be your problem.


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im sorry about your mother. She was a terrible attrative woman.

Get back to nature; hunting burgers and gathering fries.


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OfflineAkira
CosmicConsciousness
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Registered: 12/30/05
Posts: 2,283
Loc: Hay Un Mundo Mas Alla
Last seen: 11 years, 7 days
Re: contamination problems!!!! [Re: skeletor]
    #5438580 - 03/24/06 02:31 PM (17 years, 10 months ago)

I was going to say the same thing. Maybe the syringes themselves are contaminated. Have they all been from the same batch?


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Orissa India Bulk Grow (Tub Tek)
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"Our intention is our eternal fingerprint in the universe."

We know that God is good, and so are hamburgers and hot dogs. We know that hamburgers and hot dogs definitely do exist, so then by deduction of logic God too must also exist. Hamburgers + Hot dogs = God.... Duh


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Offlineredshadow
Yaddia Holla?
I'm a teapot User Gallery

Registered: 09/28/04
Posts: 282
Loc: Zeta Reticuli
Last seen: 2 years, 9 months
Re: contamination problems!!!! [Re: skeletor]
    #5438583 - 03/24/06 02:32 PM (17 years, 10 months ago)

i flame the syring everytime i insert it in a hole, so 4 times per jar. i am using a soponcers syringe so i would hope its not from them. hopefully the other jars will be ok, or ill have to save the one that is almost done, its lik 90% colonized so hopefully i will be able to save it, im all out of spores and money!!!!! i sent a good amount of cash on my new set up and hoeping to get some of it back.ill have to try the siclicone thing, should i put silicone over the jars that re already incubaing, i afraid that when i pull of the tape, even though ill be for just a split second i might contaminate them but i would like to do what i can to make sure they dont get contaminated.


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=<{:  Its only when you've lost everything that your free to do anything.......  :}>=


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Offlineskeletor
the dude
Registered: 09/06/05
Posts: 1,235
Loc: heaven on earth
Last seen: 17 years, 4 months
Re: contamination problems!!!! [Re: redshadow]
    #5438673 - 03/24/06 03:07 PM (17 years, 10 months ago)

rig up a shitty gove box
find a cardboard box... tape plastic wrap over the top two arm holes oust the shit out of teh inside and work in there
wash hands wipe down what you put in with alcohol


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im sorry about your mother. She was a terrible attrative woman.

Get back to nature; hunting burgers and gathering fries.


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