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creamcorn
mad scientist


Registered: 03/13/06
Posts: 2,962
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need liquid honeywater myc advice
#5436013 - 03/23/06 09:20 PM (18 years, 1 month ago) |
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Ok.. I had an exceedingly OLD spore print (as in from the 80s!) that I didn't really think would do anything, so I figured I might as well do some experimentation. Anyway, I made up a standard MEA plate as sort of a control experiment, and to my surprise got some growth - it was slow to start but it worked! After seeing that, I thought I'd try my hand with the liquid honeywater tek... made up two jars, one with a low concentration of honey and one with a higher concentration of honey.
In both jars I noticed a bit of sediment after PC'ing... fine brown/yellowish specs at the bottom of the jar - brings me to my first question, is that normal or was there crap in my honey that shouldn't have been? (Wish I hadn't loaned my camera out or I'd post pics)
Its been about 9 days now, and I'm getting nothing in the higher concentration jar. The lower concentration jar however I'm not sure what I'm getting - I don't know what myc should look like in liquid (only found one picture on the site here that hasn't helped), and I don't know what a contam would look like either. See, all the little sediment specs are now stuck together in a snotty looking glob - they're all still loose and free in the high concentration jar. If I swirl it around it moves really quite like you'd expect a booger to move around in a jar of water. Looking really close, it seems stringy (rhizo growth?), it tends to stick to the bottom unless i swirl it loose, and I see it give off little tiny bubbles on occasion - a sign that something's alive down there. There's also lots of little white specs that float throughout the liquid (more myc?) The big glob is still that yellowish brown color though, but it could appear so because of all the sediment trapped in it. What do you think I got happening in there? Would myc "anchor" itself together in such a way when given the honey sediment to attach to? And what do you suppose I do with it? I can't really suck it up in a syringe, its about the size of a half-dollar coin, curious if I fish it out with an innoc loop and drop it in some substrate it'd do something?
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creamcorn
mad scientist


Registered: 03/13/06
Posts: 2,962
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Re: need liquid honeywater myc advice [Re: creamcorn]
#5436043 - 03/23/06 09:29 PM (18 years, 1 month ago) |
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After posting this and getting a list of "related posts" I think I answered my own sediment question. So now just wondering if its normal that myc anchors to that sediment and ends up globbing all together...
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Darkenshroom
PsychedelicExplorationist


Registered: 02/24/06
Posts: 683
Loc: I don't exist on this pla...
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Re: need liquid honeywater myc advice [Re: creamcorn]
#5436530 - 03/23/06 11:16 PM (18 years, 1 month ago) |
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In my limited experience with honey water and that of a friend of mine. Yes Mycelium actually does aggressively eat up the sediment first, I haven't worked with anything but Karo and Honey so have little comparison. But, the way that honey colonizes is odd. I will let my friend know of this thread and he can tell you more about how his Myc ate the sediment in his honey first as well.
Darken *smiles*
PS- Grats on success with germination of a 20 something year old print.
-------------------- ~The first and most important step in cultivation of the wonderful mushroom, is the cultivation of patience for without it you doom yourself to failure~
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Herbus
...

Registered: 10/19/04
Posts: 1,477
Loc: Reading the map...
Last seen: 10 years, 4 months
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Re: need liquid honeywater myc advice [Re: Darkenshroom]
#5436985 - 03/24/06 02:29 AM (18 years, 1 month ago) |
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I just started a bunch of honey jars myself... here's what I noticed:
The jars with higher concentrations of honey (1/2 pints) are actually moving quicker than the thinner solutions.
They have very weird growth patterns but one distinctive feature is still present; rhizomorphic mycelium. As long as you've got that rhizomorphic mycelium you're good.
I'm sold on this technique, have had high efficacy with it so far. I've even taken chunks of mycelium and dropped them into the solution which, apparently, is quite effective. Every jar is displaying mycelial growth.
Good shit.
-------------------- ...
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creamcorn
mad scientist


Registered: 03/13/06
Posts: 2,962
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Re: need liquid honeywater myc advice [Re: Herbus]
#5437944 - 03/24/06 11:19 AM (18 years, 1 month ago) |
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Well just in the 12 or so hours since last night the glob has suddenly doubled in size, and its definitely mycelium! (Ironic of course I wait 9 days to ask, then suddenly it becomes obvious!) White and stringy... still looks like boogers haha.
I'm excited to see these ~20 year old spores come to life like this. Its a mystery strain too... know its a cube but thats all. We'll see what happens!
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