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Offlineb0ngman
Stranger
Registered: 10/31/05
Posts: 64
Last seen: 11 years, 5 months
Re: casing layer not colonizing [Re: b0ngman]
    #5450767 - 03/28/06 10:43 AM (17 years, 10 months ago)

mabye my contams were due to the fact that i didn't pasteurize or do anything to sterilize the 50/50 mix? next time im going to have to do that


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OfflineHotnuts
old hand
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Registered: 02/26/05
Posts: 3,436
Loc: Wild Blue Yawnder
Last seen: 25 days, 16 hours
Re: casing layer not colonizing [Re: b0ngman]
    #5451027 - 03/28/06 11:58 AM (17 years, 10 months ago)

Quote:

b0ngman said:

here is 2 of the 3 casings that got badly contamed when they were put in the warm closest (about 80F). What conditions could have sparked this? what contam is this?




80 degrees for starters and a lack of frequent air exchange. Trichoderma and other menacing molds love warm, moist and stagnant air enviroments. You need to up the air exchange it appears and get those contaminated projects away from your healthy ones. The green you see on this mold are zillions of spores.


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Offlineb0ngman
Stranger
Registered: 10/31/05
Posts: 64
Last seen: 11 years, 5 months
Re: casing layer not colonizing [Re: Hotnuts]
    #5451081 - 03/28/06 12:12 PM (17 years, 10 months ago)

i thought that when you let the cases incubate, they are in the vegatative growth stage and you do not want to check on them and let any fresh air in. You want the incubating cases to thrive off the carbon dioxide, am i mistaken?? I also thought 80F was a prime temp for incubating cases?


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Offlineb0ngman
Stranger
Registered: 10/31/05
Posts: 64
Last seen: 11 years, 5 months
Re: casing layer not colonizing [Re: b0ngman]
    #5452744 - 03/28/06 07:49 PM (17 years, 10 months ago)

so was i supposed to give the cases fresh air while incubating??? I am confused


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InvisibleDIRTYMAN
Jesusdon'tcomethrough thecotton.
Female
Registered: 11/18/05
Posts: 18,558
Loc: CZ NIGGUH
Re: casing layer not colonizing [Re: b0ngman]
    #5452771 - 03/28/06 07:55 PM (17 years, 10 months ago)

Not fresh air exchange, I think it's called passive air exhange. As long as you have some polyfill/tyvek/other filtered hole to let excess CO2 get pushed out of you're fine. You don't need a fan or anything.


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I'm racist.                http://k-k-k.com/


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OfflineHypercube
80 SRM
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Folding@home Statistics
Registered: 12/18/05
Posts: 814
Last seen: 11 years, 22 days
Re: casing layer not colonizing [Re: DIRTYMAN]
    #5452902 - 03/28/06 08:25 PM (17 years, 10 months ago)

K so to JACK this guy's thread (sorry), I have a casing problem of my own.
It's been a looooong time incubating this one casing, I think the moisture was too high and there was too much air exchange during the casing run.  I think I cased it close to 3 weeks ago  :crazyeyes:

I initiated pinning conditions 'bout a week ago, cuz I had lost patience.  About 1-2% of the casing had myc popping through.  Now, I am thinking of thinning out the casing layer (i.e. taking 1/4" off) and keep up the pinning conditions, so that primordia can fruit on the actual block of substrate.

Is this an OK idea?


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Invisiblemungojerry
free as a bird
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Registered: 02/25/06
Posts: 3,598
Loc: Nappy Dub
Re: casing layer not colonizing [Re: Hypercube]
    #5452942 - 03/28/06 08:34 PM (17 years, 10 months ago)

dude this thread has just explained what happened to me when i tried casing WOULDNT COLONIZE!!!!!


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Offlinehyphae
born to grow
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Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
Trusted Cultivator
Re: casing layer not colonizing [Re: Hypercube]
    #5452961 - 03/28/06 08:38 PM (17 years, 10 months ago)

That will work if you don't have the patience. In the mean time read my pinning strategy it will help you next time. BTW you don't want any air exchanges during the casing run the moisture is fine as long as it's not saturated. Optimal temps also play a huge roll in whether your playing the wait game or not. GL


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Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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OfflineHypercube
80 SRM
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Folding@home Statistics
Registered: 12/18/05
Posts: 814
Last seen: 11 years, 22 days
Re: casing layer not colonizing [Re: Hypercube]
    #5452999 - 03/28/06 08:49 PM (17 years, 10 months ago)

Yeah, casing was saturated on that one....just fucked it up, and have to move out soon.

Gonna take the casing off it, hopefully I get a flush from it, was a good 10 lbs of substrate...


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