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Offlinejamman
SCI fan

Registered: 12/14/05
Posts: 280
Last seen: 16 years, 10 months
Is an LC considered 2nd generation?
    #5433707 - 03/23/06 10:07 AM (17 years, 10 months ago)

So if spores are the first generation, would that make LC's from spores a second generation?

Could I still do a G2G from colonized LC jars for another generation?


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Offlineblackout
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Registered: 07/16/00
Posts: 5,266
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Re: Is an LC considered 2nd generation? [Re: jamman]
    #5434283 - 03/23/06 12:32 PM (17 years, 10 months ago)

I would consider and LC the same as getting a large MS agar wedge, chopping it up and dumping it in a jar.

G2G from a LC jar should be no problem at all.


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OfflineSolidcell
tolerance++;
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Registered: 01/20/06
Posts: 754
Loc: Memphis & 53rd
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Re: Is an LC considered 2nd generation? [Re: blackout]
    #5434378 - 03/23/06 12:57 PM (17 years, 10 months ago)

you can't do a G2G with an LC, there's no grain in a liquid culture to transfer... Making a liquid culture syringe isn't related to G2G. Spore germinate into mycellium in a jar of say WBS as they do in an LC, it's the same mycellium. You can use the LC to transfer myc as you would transfer myc with G2G (one with and one without the syringe of course). the myc would be the same 'generation' if done either way.


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Psychedelic Jar Project

"It is dangerous to be right when the government is wrong." -Voltaire

"Everything that I accepted as being true up to now I acquired from the senses or through the senses. However, I have occasionally found that they deceive me, and it is prudent never to trust those who have deceived us, even if only once." -Descartes


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Offlinejamman
SCI fan

Registered: 12/14/05
Posts: 280
Last seen: 16 years, 10 months
Re: Is an LC considered 2nd generation? [Re: Solidcell]
    #5434388 - 03/23/06 01:01 PM (17 years, 10 months ago)

I meant g2g with a jar that was inoculated with liquid culutre. Sorry for any confusion


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InvisibleRoadkillM
Retired Shroomery Mod
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Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: Is an LC considered 2nd generation? [Re: jamman]
    #5434404 - 03/23/06 01:04 PM (17 years, 10 months ago)

Quote:

jamman said:

I meant g2g with a jar that was inoculated with liquid culutre. Sorry for any confusion




your confusing me!~

do you mean...transfering an LC to a new LC jar?


g2g = grain to grain transfer.


tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineSolidcell
tolerance++;
Male

Registered: 01/20/06
Posts: 754
Loc: Memphis & 53rd
Last seen: 15 years, 4 months
Re: Is an LC considered 2nd generation? [Re: Solidcell]
    #5434418 - 03/23/06 01:08 PM (17 years, 10 months ago)

Yes, you can inoculate a jar with an LC and then when that jar colonizes, yes you can do a G2G to another jar. But why not just use more of the LC?


--------------------
Psychedelic Jar Project

"It is dangerous to be right when the government is wrong." -Voltaire

"Everything that I accepted as being true up to now I acquired from the senses or through the senses. However, I have occasionally found that they deceive me, and it is prudent never to trust those who have deceived us, even if only once." -Descartes


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Offlinemikeownow
Humungus fungus

Registered: 09/01/05
Posts: 2,856
Loc: WA,USA
Last seen: 17 years, 3 months
Re: Is an LC considered 2nd generation? [Re: Solidcell]
    #5434500 - 03/23/06 01:32 PM (17 years, 10 months ago)

GTG is stupid use some Hpoo like I am trying


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No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.


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Offlinejamman
SCI fan

Registered: 12/14/05
Posts: 280
Last seen: 16 years, 10 months
Re: Is an LC considered 2nd generation? [Re: jamman]
    #5434506 - 03/23/06 01:34 PM (17 years, 10 months ago)

Start an LC with a syringe(1), let colonize, inject into grain jars(2). Let those colonize and transfer to more grains(3). Would the last batch of grains (3) be considered the third generation or second generation?


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Common Misspelled Words


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InvisibleRoadkillM
Retired Shroomery Mod
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Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: Is an LC considered 2nd generation? [Re: jamman]
    #5434516 - 03/23/06 01:37 PM (17 years, 10 months ago)

a second generation.


tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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InvisibleCaptainKirk

Registered: 07/15/05
Posts: 2,478
Loc: gone
Re: Is an LC considered 2nd generation? [Re: mikeownow]
    #5434518 - 03/23/06 01:37 PM (17 years, 10 months ago)

uhhhh  g2g is great...i dont know where you get that info but it is wrong..i i g2g jars all the time ...its quick and easy ..if you can work sterile :wink:LC's rock as well
Quote:

GTG is stupid use some Hpoo like I am trying




Edited by CaptainKirk (03/23/06 01:40 PM)


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OfflineIGnosticAbhorI
Stranger-er

Registered: 11/06/04
Posts: 4,899
Last seen: 8 months, 5 days
Re: Is an LC considered 2nd generation? [Re: mikeownow]
    #5434521 - 03/23/06 01:38 PM (17 years, 10 months ago)

G2G is very effiecent when dealing with lots of jars...And it's really great when you hate messing around with needles...What does hpoo have to do with inoculation? lol. Hpoo is used in bulk grows..

:confused:

-Gnostic


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Offlinejamman
SCI fan

Registered: 12/14/05
Posts: 280
Last seen: 16 years, 10 months
Re: Is an LC considered 2nd generation? [Re: mikeownow]
    #5434538 - 03/23/06 01:44 PM (17 years, 10 months ago)

Yeah man, g2g is awesome. It and LC are amazing ways to produce incredible amounts of spawn. I think its stupid not to.


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Common Misspelled Words


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OfflineLouddrum
Stranger
Registered: 03/10/06
Posts: 80
Last seen: 16 years, 9 months
Re: Is an LC considered 2nd generation? [Re: jamman]
    #5434555 - 03/23/06 01:48 PM (17 years, 10 months ago)

Why should horse poo be only for bulk grow's.......or are you just stating the obvious that's it's used in bulk grow's?


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