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Offlinesplifner180
Student
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Registered: 03/24/06
Posts: 1,288
Loc: USA, East Coast
Last seen: 1 year, 3 months
Re: my setup [Re: ssjwizard]
    #5471559 - 04/02/06 05:39 PM (17 years, 9 months ago)

Hey Porksoda, I'm pretty new so forgive me if this is a stupid question.  :wink:

What is LC?

splif


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First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub
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OfflineShroomInduced
I read too manymycology books
Male

Registered: 04/19/05
Posts: 309
Loc: A giant mushroom in my he...
Last seen: 12 years, 5 months
Re: my setup [Re: splifner180]
    #5471592 - 04/02/06 05:48 PM (17 years, 9 months ago)

Liquid Culture. Its really easy to make as well. Just take a jar, put a hole in it, add a cup of water, add a teaspoon of honey or karo with vanilla or vanilla, and then pc at 20psi for 15 minutes, take out let cool, hit it up with some spores, in about 5 days you should have myc growing in it. Suck some of that up in a syringe, shoot it in a jar, 1-2 days later the jars colonize.


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That's a deep kiss too, like the Europeans. You know, the French, they have to unhinge their jaw to show love.

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Offlinessjwizard
Stranger
Registered: 09/01/05
Posts: 206
Last seen: 15 years, 17 days
Re: my setup [Re: ShroomInduced]
    #5566227 - 04/28/06 08:25 AM (17 years, 9 months ago)

ok so last jars stalled and were discarded. ive determined that my syringe was not bad but simply quite weak, either there werent enough spores or they had low germination rate. i determined this by using 3ml of solution(leaving me with just under 5ml) to start a LC jar. this is about 2 wks old and it seem to me that it should be further along but theres definatly a good volume of mycelium in it. 3 days ago i prepaired 2 new jars and 2 days ago i injected the remaining spore solution i had into them. i am currently boiling 2 more jars made using the coffee method. prepaired acording to PF tek for simple minds using 1/2 coffee 1/2 water inplace of just water. im going to inoculate these jars either late tonight or tomarrow with the LC that ive got made up. so hopfully in a few weeks i should have some nice jars of mycelium growing and ill finaly get a chance to use my fruiting chamber.


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All statments, Imagery, and Ideas contained therin any postings are meerly retold sotrys of fictional acts created by a figmant of the universe imagination.


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Invisiblecreamcorn
mad scientist
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Registered: 03/13/06
Posts: 2,962
Trusted Cultivator
Re: my setup [Re: ssjwizard]
    #5566364 - 04/28/06 09:21 AM (17 years, 9 months ago)

outa curiosity why the hell have you gathered so much stuff and equipment, to knock up *2* jars at a time? :smile:  how do you plan on filling three casing trays like you mentioned from a lonely 2 jars?  they are half pinters right?  on a friend's first grow he got 3 dozen jars, made 4 LC's from a small portion of a single syringe, knocked up 30 (10 at a time, spaced out with a week between so they wouldn't all finish at once), and kept 2 empties around just in case there were other uses for them later.  oh boy did the jars just keep on coming.  as the first jars cakes finish fruiting, the last jars are ready to go on, and a nice continuous cycle is thus made :smile:

and your jars didn't stall because of spores.  if you have a bad or dilute spore syringe, they simply wont start.  if they started, and stalled, its something else.  if you're using jars larger than 1/2 pints, theres a good chance thats why.  if you kept your air holes tightly covered, that's another reason why.  if they were exposed to excessive heat at some point, there's yet another possibility.  if they were infected by bacteria, they'd smell, and stall... well you get the picture, there's lots of other reasons why jars stall.  especially considering your LC worked, you just proved the spores are fine.

I think you're making an incorrect assumption that its the spores, need to take a closer look here and find out what the real reason was so it doesn't happen again.

a better idea might be to suck up that LC you got going.  get as much myc as you can into the syringe.  if you see some floaties in there you're good.  shake like mad.  use something like 2-3cc per 1/2pt jar spread between the 4 innoc points.  go for more jars, 10 or 12.  it will take 2-3 syringes worth of LC to get them all.  you'll notice growth just explode within 3-4 days... especially when adding coffee ive seen full colonization in as little as 10 days (but IMO 25% coffee to 75% water is more ideal.  coffee is acidic, and so are myc waste products.  too much coffee means too acidic an environment towards the end of your colonizing, and slowed or stalled growth right near the very end.)

good luck.


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Offlinessjwizard
Stranger
Registered: 09/01/05
Posts: 206
Last seen: 15 years, 17 days
Re: my setup [Re: creamcorn]
    #5569447 - 04/29/06 01:43 AM (17 years, 9 months ago)

i have all this stuff that i INTEND on using to its fullest AFTER i get my methods locked in. the 2 jars at a time are simply so that i dont waste to much material in failure. and your right my last jars didnt stall they never began i looked at pictures i took of them and what i beleived to be mycelium that stalled i further examined and it was just wet clumps of BRF. i have 2 dozen jars that ill be able to use once i actualy get up and going im just tring to be cautous with my startup beacouse id rather use my money for something else since i allready spent as much as i need to for several full grows. i just inoculated the 2 coffee jars with LC about 6 ml each beacouse my LC looks very sparse compared to some images ive seen here on the shroomery. im aware that its easy to excede the moisture level in the jars and i took into account that i would be doing this when prepairing the jars they had just enough for the verm brf to be properly prepaired but i put 1/4 inch of dry verm on the bottom of the jar so that the excess moisture will have someplace to go so its not pooling. these jars i went with 5 inoculation points using about 1ml in each point. i hope to see something happening with the lc/coffee jars in about 3-4 days. anyways thanks for the reply hope to talk to ya more in the future.


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All statments, Imagery, and Ideas contained therin any postings are meerly retold sotrys of fictional acts created by a figmant of the universe imagination.


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