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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Vermiculite

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OfflineShroomNewb
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Registered: 11/09/01
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Failure
    #542590 - 02/06/02 04:08 PM (14 years, 9 months ago)

I think I have found out the reason why I have failed twice now.

I don't use tape on the holes of my jars. I use tinfoil, but the verm layer always seems to be damp or even soaked. Is this my problem?


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OfflineSeussA
Error: divide byzero

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Registered: 04/27/01
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Re: Failure [Re: ShroomNewb]
    #542600 - 02/06/02 04:16 PM (14 years, 9 months ago)

Without a lot more information there is no telling. If you are having problems, then you should follow one of the easier teks verbatim. Don't start to change things until you have done them all correctly.

The analogy I like to use is baking. I used to have loads of problems getting bread to turn out... it would always rise too much while baking and turn out awful. One time when I was showing somebody what happens, they asked why I wasn't adding any salt. Well, I wanted healthy low-salt bread. That was when I found out that salt helps inhibit the growth of the yeast and prevents the bread from rising too much while baking. I had always assumed the salt was just for taste.

What I am getting at here, is the teks spell out things for a purpose. If they say to use tape, use tape. Until you understand all the parts of the tek, don't go changing things. Most likely your changes won't work and you will wonder why it failed.

Peace


--------------------
Just another spore in the wind.


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Invisibleangryshroom
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Re: Failure [Re: ShroomNewb]
    #542616 - 02/06/02 04:28 PM (14 years, 9 months ago)

Hey bro,

I use tin foil on my jars. You have to be certain however that they are TIGHT and well secured on the jars, before you throw them in the pot or PC. If your using a pot of boiling water, (which I use), you have to make sure the water level is not how high they tell you to put it (which is half way up the sides of the jars)...I usually only put like 1 1/2 inch of water, put a washcloth on that, then set my jars on top. Make sure there is int any water splashing up into the tin foil.

What do you mean by failing, no growth, or contams?


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OfflineShroomNewb
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Registered: 11/09/01
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Re: Failure [Re: angryshroom]
    #542727 - 02/06/02 06:21 PM (14 years, 9 months ago)

First try was slow growth, then contams, second time was hardcore contams.

Hehe.

I'm just asking this cause some teks say use tape, and some don't. Just asking if it is normal to get water in your jars or not?

Btw, it could just be steam thats getting under the tinfoil into the jars, I dunno, I only use a couple inches of water in my pressure cooker.


Edited by ShroomNewb (02/06/02 06:23 PM)


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Invisibleangryshroom
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Re: Failure [Re: ShroomNewb]
    #542827 - 02/06/02 08:15 PM (14 years, 9 months ago)

Yeah true. I guess using some masking tape wouldn't hurt ya.
I should try it sometime...makes sense.



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Offlinejag192
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Re: Failure [Re: ShroomNewb]
    #542871 - 02/06/02 09:10 PM (14 years, 9 months ago)

Have you considered that you may be getting contams when you inoculate and not from the foil or sterilization process. How do you keep everything sterile while you inoculate? This seems more likely to me than anything else as it is the one time your substrate is exposed. Just my two cents hope you have better luck next time...don't give up!!!!


--------------------
"The man of Tao remains unknown
Perfect virtue produces nothing
'No-Self' is 'True-Self'
And the greatest man is Nobody"


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OfflineUrbanistiC
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Re: Failure [Re: jag192]
    #542995 - 02/06/02 11:19 PM (14 years, 9 months ago)

I am taking a guess in the dark, but I bet if you use the oven tek or a hood for the innoculation process (with latex gloves on, and heat the needles real good) then you might get better results. Just my 2 pennies.


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Keep it ReaL.


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Offlinebassplayer74
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Registered: 11/24/01
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Re: Failure [Re: ShroomNewb]
    #543005 - 02/06/02 11:27 PM (14 years, 9 months ago)

Maybe you're not sterilizing your substrate completely? Do you use a pressure cooker? If not, that could very well be your problem. If you just use a big pot, then let it boil longer. And make sure no water or steam gets into your jars during the process. And definitely use the oven tek. It's the only way to fly...bassplayer74


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If you will it, it is no dream.


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OfflineShroomNewb
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Registered: 11/09/01
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Re: Failure [Re: bassplayer74]
    #545069 - 02/08/02 05:52 PM (14 years, 9 months ago)

I do the brf method, I PC it at 15psi for 1 hour, I use the oven tek, I use sterilizing hand soap, mad lysol, I wear a face mask, hairnet, I use rubbing alcohol, I flame the needle, and I regularl have showers.

My room is quite dirty, although they should be ok in my room because they are in the jars with the verm layer, in a sealed container, and when I look at them I spray lysol right?

The verm layer isn't doing it's job if it is wet though, am I right? dampness attracts contams..

I am getting down about this although I'm not about to quit. I already have invested over 200 bucks into all my shit, and I'm not about to throw all of that money away without a little payoff.


Edited by ShroomNewb (02/08/02 06:38 PM)


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Offlinetomatofarmer
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Registered: 01/10/02
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Re: Failure [Re: ShroomNewb]
    #545118 - 02/08/02 07:02 PM (14 years, 9 months ago)

maybe its your syringes...get them from a good vendor?i use the same method and i get no contams..i got 30 jars now and not 1 contam...all at about 50%-90% colonized....
i use tape...but i sterilize my needle with flame and wipe with alcahol, and insert strait through the tape into the hole..i resterileize evry jar to keeo from cross com...i dont recover the holes at all..incobate them with holes *punctured* and covered with double layered tin foil.....just my 2 cents
-Lee
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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Vermiculite

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