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endlesswinter77
Nomadic Herdsman


Registered: 11/09/05
Posts: 106
Loc: Nor Wes
Last seen: 12 years, 1 month
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LC question
#5424303 - 03/21/06 01:13 AM (17 years, 10 months ago) |
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I recently inoculated two jars of liquid culture made with powdered malt extract. I dissolved the powder completely to begin with, and used about 1 Tablespoon to 1 cup water. Now there is a grimey lookin sedimentation in the bottom. Anything to worry about?
-------------------- There's some things a man has to prove to himself alone... not to anyone else.
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cappa
Nerd
Registered: 02/12/06
Posts: 854
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Probably overcooked your LC. Make a new one and PC it for a little less time.
-------------------- Their are 10 types of people. Those that understand binary, and those who don't. ~Cappa.
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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>>>>powdered malt extract<<<<<<<
What kind? I hope it wasn't for making malted milk.
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Teon
Chemist/Self-AppointedFAQ Facist


Registered: 03/16/02
Posts: 321
Loc: Here
Last seen: 15 years, 1 month
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While Cappa is most likely correct concerning the impetus of your issue, I would contend (on the basis of the experience of SWIM) that it will be fine.
-------------------- No statements made in any post or message by myself should be construed to mean that I am now, have ever been, or will at any future time will be participating in or considering participation in any activities in violation of any legal statue; be it local, state, or federal law. All posts are works of fiction, and are intended for entertainment purposes only. Don't just assume anything I or anyone else says is true. Test it for yourself. See if it's true. Generate more useful information. The world is running desperately low on it.
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Wronguy
Registered: 03/05/05
Posts: 4,450
Last seen: 12 years, 7 months
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Re: LC question [Re: agar]
#5424472 - 03/21/06 07:55 AM (17 years, 10 months ago) |
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You used too much malt extract. A tablespoon is about 10 grams. I never use more than 10 grams per 4 cups of water and get some really nice, thick liquid cultures. I have found, in most cases, that the grimy sedimentation is mycelium feeding on the bottom. An excessive amount of malt extract can easily discolor your colonizing mycelium and give the false impression of a contaminate.
Next time I recommend using 10 grams of light malt extract and 10 grams of dextrose to every 4 cups of water. The results speak for themselves.
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endlesswinter77
Nomadic Herdsman


Registered: 11/09/05
Posts: 106
Loc: Nor Wes
Last seen: 12 years, 1 month
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Re: LC question [Re: Wronguy]
#5425666 - 03/21/06 02:52 PM (17 years, 10 months ago) |
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Right on. thanks for the feedback, i do think that I put in too much extract, and could possibly have overcooked it also. I recently checked the jars, and the sedimentation has turned into larger thicker more translucent clumps, so i think the mycelium is growing down there. Since it could give the false impersonation of a contam.... I could try it out on say a cake and if it grows its not contaminated right?
-------------------- There's some things a man has to prove to himself alone... not to anyone else.
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