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OfflineCrackalack
Stranger
Registered: 08/14/05
Posts: 38
Last seen: 17 years, 3 months
H. Poo Contam.
    #5423006 - 03/20/06 07:42 PM (17 years, 10 months ago)

Is it true that injecting LC into pasturized H. Poo will most likely cause contams? If this is true what is a good substrate for LC? I thought one of the main advantages of using H.Poo is because it is a great source for shroom nutes.


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InvisibleMindsEye
AMT Fiend
Male

Registered: 02/20/06
Posts: 645
Loc: cerebral cortex
Re: H. Poo Contam. [Re: Crackalack]
    #5423046 - 03/20/06 07:51 PM (17 years, 10 months ago)

Anything is good for LC's think of it as spores that have germinated, poo cakes, wbs, rye, pf jars they are all good for LC.


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Easy Poo Cake Tek


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InvisibleSwan Song
Registered: 02/08/06
Posts: 559
Re: H. Poo Contam. [Re: Crackalack]
    #5423065 - 03/20/06 07:54 PM (17 years, 10 months ago)

i dont know about the contams. but i do know you need to go to grain or cakes or something else first.
as far as contams. i would say that because your spawn ratio is so small the bacteria remaining in the hpoo is going to kill just about any chance the myc has to start growth.
there is just alot more of it in the poo.
check around for spawn ratios to substrate i think it is something like 10 to one. but dont quote me on that.


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OfflineCrackalack
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Registered: 08/14/05
Posts: 38
Last seen: 17 years, 3 months
Re: H. Poo Contam. [Re: Swan Song]
    #5423209 - 03/20/06 08:23 PM (17 years, 10 months ago)

This would be in spawn bags not cake. I don't know why it would contaminate but that is what some one said in another thread. I think it is 8 to 1 but not positive either. Ill check. Thanks guys.


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OfflineCrackalack
Stranger
Registered: 08/14/05
Posts: 38
Last seen: 17 years, 3 months
Re: H. Poo Contam. [Re: Crackalack]
    #5423242 - 03/20/06 08:30 PM (17 years, 10 months ago)

The thing is though, if you're going to grow from straight poo, then you need to completely sterilize the poo (not pasteurize) and then knock it up w/ an LC.

When using pasteurized poo, you need to use a spawn which has already been fully colonized. The use of LCs as an inoculant in pasteurized poo will generally result in contamination.

This was posted by rabid_bird or something like that. But this is where I heard it.


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Invisiblefigment
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Registered: 01/04/06
Posts: 522
Loc: Quidity
Re: H. Poo Contam. [Re: Crackalack]
    #5423431 - 03/20/06 09:17 PM (17 years, 10 months ago)

Sterilized poo is not good..contaminates VERY easy. Pasturized poo, on the other hand, can stay good for several months. Just make sure you are very, very clean when injecting your LC.


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OfflineEquilibriuM
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Registered: 07/17/05
Posts: 2,323
Last seen: 16 years, 7 months
Re: H. Poo Contam. [Re: figment]
    #5423463 - 03/20/06 09:24 PM (17 years, 10 months ago)

you need a good recipe. not just hpoo. poo/vermiculite/wbs/vegtable oil/kelp meal is a damn good one.

Here is Wronguys recipe

"kay, I'll share. Now the ingredients were compiled with the help of Blue Helix and Agar. There is nothing exotic in here, but it has a good nutritional profile. This recipe is for 4 large filter patch spawn bags. It will fill them within 2 1/2 inches of the bottom of the filter patch and lays out perfectly in 19 quart containers with a substrate depth of 3 1/2 inches.

The recipe is as follows:

48 Cups of horse manure (shredded)
24 Cups of vermiculite
16 1/2 Cups of dry WBS (do not soak or simmer)
4 Tablespoons of dry Kelp Meal
6 Tablespoons of Canola or Vegetable oil
21 Cups of water

Thoroughly mix all dry ingredients together. Now, add the water and mix again. Due to the human error factor on measurements, you may consider adding only 18 cups of water to begin with and adjust it from there. However, I have found that any measurements that are off are negligible in the moisture content.

After all the water is added, test your moisture content by giving the substrate a hard squeeze. You should only be able to extract a few drops from this, not a steady drip. If you find that your substrate is a little too wet, slowly add vermiculite until you get the right consistency. If you measure everything correctly this should not be necessary. The moisture content needs to be slightly dryer than normal to allow for the liquid culture you will inject later.

The final step is adding the oil. I prefer to add the oil in after the water has been added. I find it's much easier to mix in and doesn't stick to the substrate in one area or clump. Mixing the oil in with the dry ingredients will surely clump on you.

Load your spawn bags at the same time and make sure they are as even as possible. Now it does not matter where you mix this. I do mine in my garage. I know I'm going to sterilize the substrate so contaminates are less of a concern. Follow the tek by Blue Helix to complete it. I personally PC for 3 1/2 hours at 15 PSI.

After they have cooled just inoculate and wait. "


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HELP!!!!!!!!!


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