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Offlinediscountpass
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LC PROBLEMO w/pics
    #5422804 - 03/20/06 07:06 PM (17 years, 10 months ago)

ok, my Lc is kinda caramel color, ive seen in pics that the myc in the LC is white. any one have any comments? should i go ahed and inject?







Blah! i unno, looks yellowish


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InvisibleMindsEye
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Re: LC PROBLEMO w/pics [Re: discountpass]
    #5422854 - 03/20/06 07:15 PM (17 years, 10 months ago)

what did you make the LC out of, karo, honey, malt dex?


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Offlinediscountpass
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Re: LC PROBLEMO w/pics [Re: MindsEye]
    #5422934 - 03/20/06 07:30 PM (17 years, 10 months ago)

Honey.

please tell me it will be okay doctor!?>!?!


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Offlinemogur
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Re: LC PROBLEMO w/pics [Re: MindsEye]
    #5422964 - 03/20/06 07:36 PM (17 years, 10 months ago)

Yeah, it's kind of important to know how cloudy the solution was when you inoculated. Bacterial contams in LCs turn the solution cloudy. If you went from clear to cloudy, that indicates a problem. If you started cloudy, then no indication. Biologists use turbidity measurements to calculate bacterial growth in liquids. Order of magnitude determination can be discerned by eyeball, spectrography is used for accuracy and identification of wavelengths associated with specific bacteria.


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Offlinediscountpass
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Re: LC PROBLEMO w/pics [Re: mogur]
    #5422972 - 03/20/06 07:37 PM (17 years, 10 months ago)

its always been kinda cloudy, never was white. i guess all i can do is inject 5 jars and see what happens ? :/


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InvisibleHolydiver
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Re: LC PROBLEMO w/pics [Re: discountpass]
    #5422973 - 03/20/06 07:37 PM (17 years, 10 months ago)

If it looks that rough right out of the PC, you cooked too long and carmelized your solution. How long are you PC'ing?


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InvisibleMindsEye
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Re: LC PROBLEMO w/pics [Re: mogur]
    #5422980 - 03/20/06 07:38 PM (17 years, 10 months ago)

since you used honey this is questionable, really the only way to tell at this point is make a couple of "control jars" they are just tester jars to see if LC is contamed, if they all contaminate at the inoculation points then you know theres a problem with the LC.


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Offlinemogur
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Re: LC PROBLEMO w/pics [Re: discountpass]
    #5422992 - 03/20/06 07:40 PM (17 years, 10 months ago)

Honey? Oops. That is probably bacteria, then. If you are already carmelizing the honey, then you don't want to increase PC time. Try again, and pay real close attention to aseptic methods during inoculation.


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Offlinediscountpass
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Re: LC PROBLEMO w/pics [Re: Holydiver]
    #5422999 - 03/20/06 07:41 PM (17 years, 10 months ago)

30mins, if tis caramelized, can i still inject? or start again


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Offlinediscountpass
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Re: LC PROBLEMO w/pics [Re: discountpass]
    #5423010 - 03/20/06 07:43 PM (17 years, 10 months ago)

judging by the pictures u see, how do they look? im going to try the test jar right now, if its not contam how long till i see results?


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InvisibleHolydiver
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Re: LC PROBLEMO w/pics [Re: discountpass]
    #5423033 - 03/20/06 07:48 PM (17 years, 10 months ago)

30 mins is too long. Try 15 minutes next run. Personally, I wouldn't waste spores on a carmelized LC (tried that w/ no luck).


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InvisibleMindsEye
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Re: LC PROBLEMO w/pics [Re: discountpass]
    #5423034 - 03/20/06 07:48 PM (17 years, 10 months ago)

I wouldnt waste the spore on it, make another one pc for 15- 20 mins.


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Offlinediscountpass
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Re: LC PROBLEMO w/pics [Re: MindsEye]
    #5423094 - 03/20/06 08:02 PM (17 years, 10 months ago)

im going to try again.


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Offlinemikeownow
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Re: LC PROBLEMO w/pics [Re: discountpass]
    #5423469 - 03/20/06 09:25 PM (17 years, 10 months ago)

I use a lot of honey in myn and some carmelises but my lc's are always ok but I only pc for 15-25 minutes my latest one I pced for only 13


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Invisiblecappa
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Re: LC PROBLEMO w/pics [Re: mikeownow]
    #5424350 - 03/21/06 02:03 AM (17 years, 10 months ago)

My first honey LC owned me. Seriously, it kicked my ass.
Try and try again! GL!


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Offlinediscountpass
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Re: LC PROBLEMO w/pics [Re: cappa]
    #5424657 - 03/21/06 10:41 AM (17 years, 10 months ago)

ok, i just PC'ed the lc w/honey lasrt night for 15 mins and i look @ it this moring and it looks caramel/cloudy again. WTF am i doing, im going to try corn syrup. f*ck honey! :evil:


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OfflineBlueBruiser
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Re: LC PROBLEMO w/pics [Re: discountpass]
    #5424870 - 03/21/06 11:33 AM (17 years, 10 months ago)

Sediment forms after you PC with honey, malt/dextrose and im not sure about corn syrup. The cloudyness is from the sediment I would think. Thats normal. You can boil the water first then add the honey to make some of the sediment form then you can filter the water with some coffee filters, that might clear it up a bit. Also caramelization occurs when it gets too hot in your PCer. The Mushroom cultivation book by Paul Stamets recommends not exceding a temp of 250F in the PCer or else the sugar caramelizes. At 15psi the temp in the PCer is 250 so as a precautionary tip you can PC at 12psi for say 20 min. Just dont exceede 250F and your good.


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Offlinemogur
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Re: LC PROBLEMO w/pics [Re: BlueBruiser]
    #5424963 - 03/21/06 11:54 AM (17 years, 10 months ago)

Light karo is glucose/fructose and makes an excellent LC, mainly because of its clarity. Don't worry about the vanilla flavoring in it, either, because some people use vanilla by itself for LCs. A 1% solution takes off the fastest, but grinds to a halt after a few days at the flaky stage, before forming into a ball, and is subject to bacterial infection. Greater than 5% solution is really slow to get started, protects against bacterial growth because of osmotic pressure of the sugar concentration, and keeps growing to very dense colonization of the culture. 3-4% is a good compromise for fast and full colonization. That's about a Tablespoon per pint of water. But don't get all uptight about measuring, it just isn't that critical.


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InvisibleliloldmeFacebook
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Re: LC PROBLEMO w/pics [Re: mogur]
    #5425003 - 03/21/06 12:09 PM (17 years, 10 months ago)

try boiling instead of pc... just a thought...


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