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Invisibleohmatic
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peroxide agar
    #5418840 - 03/19/06 01:42 PM (18 years, 2 months ago)

round two.

when using peroxide agar i remember reading that the mycelium breaks down the h2o2
when its growing on the media.

what i wonder is, as the mycelium just grows on the top surface of the agar
i guess it only breaks down the h2o2 in the top surface aswell whereas
the below laying portion of agar still got "working" h2o2 in it.

is that correct ?


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Offlinefalcon
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Re: peroxide agar [Re: ohmatic]
    #5422795 - 03/20/06 07:04 PM (18 years, 2 months ago)

The mycelium grows into the agar and on top of the agar. It breaks down the peroxide wherever it has grown.

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Invisibleohmatic
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Re: peroxide agar [Re: falcon]
    #5424895 - 03/21/06 11:39 AM (18 years, 2 months ago)

Quote:

falcon said:
The mycelium grows into the agar and on top of the agar. It breaks down the peroxide wherever it has grown.




yes i did understand that, but im interested into knowing if i cut a wedge of peroxide agar,
is just the h2o2 on the top surface where the mycelium grew destroyed
or did it manage to destroy all of it in the entire wedge (such as bottom/sides)


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OfflineRogerRabbitM
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Re: peroxide agar [Re: ohmatic]
    #5425166 - 03/21/06 12:51 PM (18 years, 2 months ago)

Peroxide breaks down very fast in the presence of organic matter. I would imagine all of the h2o2 was broken down long before the mycelium finished colonizing the top of the dish.
RR


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