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Babo911
Pooper Scooper


Registered: 09/05/05
Posts: 1,745
Loc: Arizona Bay
Last seen: 10 years, 7 months
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Liquid Growth VS spore syringe
#5418348 - 03/19/06 10:29 AM (17 years, 10 months ago) |
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I have 2cc of B+ left and I was wondering if i should try to make a liquid culture using karo thanks to this post by road kill or should i just make a spore syringe. I make WBS jars if that plays a difference, I got a pressure cooker but never got a glove box and with the limited space in my apartment i don't plan on getting one till i move into a house. Please let me know.
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Ronhero on the Growery is me
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: Liquid Growth VS spore syringe [Re: Babo911]
#5418389 - 03/19/06 10:48 AM (17 years, 10 months ago) |
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LC, without question.
You can turn 2 ml into 10,000.
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Babo911
Pooper Scooper


Registered: 09/05/05
Posts: 1,745
Loc: Arizona Bay
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Re: Liquid Growth VS spore syringe [Re: Babo911]
#5418400 - 03/19/06 10:54 AM (17 years, 10 months ago) |
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that is what i was thinking, i just wanteda a second opinion. And if you know what should i do for a "filtered lid" i was thinking of poking a hole and covering with Tyvek over the lid but under the ring? any suggestions?
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Ronhero on the Growery is me
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stelthvue
is frightened ofpoo
Registered: 01/20/06
Posts: 272
Last seen: 17 years, 9 months
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Re: Liquid Growth VS spore syringe [Re: Babo911]
#5418405 - 03/19/06 10:55 AM (17 years, 10 months ago) |
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ive come to learn that 3M micropore tape is amazing for a filter.
-------------------- No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.
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Babo911
Pooper Scooper


Registered: 09/05/05
Posts: 1,745
Loc: Arizona Bay
Last seen: 10 years, 7 months
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Re: Liquid Growth VS spore syringe [Re: stelthvue]
#5418410 - 03/19/06 10:57 AM (17 years, 10 months ago) |
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works for me, now is there any chance i can pick it up at wal-mart, i work there so it be realy convient to get it while i am there
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Ronhero on the Growery is me
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stelthvue
is frightened ofpoo
Registered: 01/20/06
Posts: 272
Last seen: 17 years, 9 months
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Re: Liquid Growth VS spore syringe [Re: stelthvue]
#5418441 - 03/19/06 11:07 AM (17 years, 10 months ago) |
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yeah, check the medical section.
-------------------- No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: Liquid Growth VS spore syringe [Re: Babo911]
#5418443 - 03/19/06 11:08 AM (17 years, 10 months ago) |
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Quote:
Babo911 said: that is what i was thinking, i just wanteda a second opinion. And if you know what should i do for a "filtered lid" i was thinking of poking a hole and covering with Tyvek over the lid but under the ring? any suggestions?
    Small bore rubber hose from hardware, or autoparts store. RTV gasket sealer from autoparts store.
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Babo911
Pooper Scooper


Registered: 09/05/05
Posts: 1,745
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Re: Liquid Growth VS spore syringe [Re: Babo911]
#5418455 - 03/19/06 11:11 AM (17 years, 10 months ago) |
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one for air exchange the other to suck out the culture i assume? Both stuffed with polly fill. I know this is going to sound stupid but where did you get the plastic top
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Ronhero on the Growery is me
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stelthvue
is frightened ofpoo
Registered: 01/20/06
Posts: 272
Last seen: 17 years, 9 months
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Re: Liquid Growth VS spore syringe [Re: Babo911]
#5418462 - 03/19/06 11:14 AM (17 years, 10 months ago) |
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not a stupid question. ive checked a few stores and cant find plastic lids for 1/2 pint kerr jars.
-------------------- No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: Liquid Growth VS spore syringe [Re: Babo911]
#5418470 - 03/19/06 11:16 AM (17 years, 10 months ago) |
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Miracle Whip, and/or Mayo jars @ any grocery store. (recycling for a differing use is a good thing)
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figment
Apothecary


Registered: 01/04/06
Posts: 522
Loc: Quidity
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Re: Liquid Growth VS spore syringe [Re: stelthvue]
#5418472 - 03/19/06 11:17 AM (17 years, 10 months ago) |
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you can get them from Mycosupply.com
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: Liquid Growth VS spore syringe [Re: figment]
#5418478 - 03/19/06 11:20 AM (17 years, 10 months ago) |
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stelthvue
is frightened ofpoo
Registered: 01/20/06
Posts: 272
Last seen: 17 years, 9 months
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Re: Liquid Growth VS spore syringe [Re: agar]
#5418486 - 03/19/06 11:24 AM (17 years, 10 months ago) |
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sweet agar, thanks. now i wont have to throw out lids because of rust.
-------------------- No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.
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Babo911
Pooper Scooper


Registered: 09/05/05
Posts: 1,745
Loc: Arizona Bay
Last seen: 10 years, 7 months
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Re: Liquid Growth VS spore syringe [Re: stelthvue]
#5418514 - 03/19/06 11:35 AM (17 years, 10 months ago) |
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see lids are cheap for me, it is abot $1.00 for 12, but for LC i found that the lid to the now empty penutbutter jar fits great, i am soo happy
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Ronhero on the Growery is me
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Babo911
Pooper Scooper


Registered: 09/05/05
Posts: 1,745
Loc: Arizona Bay
Last seen: 10 years, 7 months
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Re: Liquid Growth VS spore syringe [Re: agar]
#5418522 - 03/19/06 11:38 AM (17 years, 10 months ago) |
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Ronhero on the Growery is me
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fireworks_god
Sexy.Butt.McDanger


Registered: 03/12/02
Posts: 24,855
Loc: Pandurn
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Re: Liquid Growth VS spore syringe [Re: Babo911]
#5418549 - 03/19/06 11:46 AM (17 years, 10 months ago) |
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So, then, one theoretically can take a spore syringe, inoculate some liquid substrate, exponentially increase the amount of solution one can then use to inoculate more jars/casings?
That is to ask, that is the purpose, right? 
 Peace.
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If I should die this very moment I wouldn't fear For I've never known completeness Like being here Wrapped in the warmth of you Loving every breath of you
Edited by fireworks_god (03/19/06 11:52 AM)
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Tweexican
Clit Commander

Registered: 11/06/03
Posts: 657
Last seen: 5 years, 6 months
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Re: Liquid Growth VS spore syringe [Re: fireworks_god]
#5418557 - 03/19/06 11:48 AM (17 years, 10 months ago) |
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well THAT, and the promise of faster colonization since the spores are already germinated.
think, g2g in liquid form
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fireworks_god
Sexy.Butt.McDanger


Registered: 03/12/02
Posts: 24,855
Loc: Pandurn
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Re: Liquid Growth VS spore syringe [Re: Tweexican]
#5418574 - 03/19/06 11:54 AM (17 years, 10 months ago) |
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I thought of that after I made my post... I assume that it is also quicker/easier for the spores to germinate in the liquid substrate than in, say, a verm/brf substrate?
Definitely something to look forward to trying out. 
 Peace.
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If I should die this very moment I wouldn't fear For I've never known completeness Like being here Wrapped in the warmth of you Loving every breath of you
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skeletor
the dude
Registered: 09/06/05
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Re: Liquid Growth VS spore syringe [Re: fireworks_god]
#5418680 - 03/19/06 12:31 PM (17 years, 10 months ago) |
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its not really exponetial. but if you are growing on a small scale it pretty much is haha
-------------------- im sorry about your mother. She was a terrible attrative woman. Get back to nature; hunting burgers and gathering fries.
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: Liquid Growth VS spore syringe [Re: skeletor]
#5418749 - 03/19/06 01:02 PM (17 years, 10 months ago) |
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  If you have/use a stir plate.
Growth is EXPONENTIAL. 1 myc strand broken in to 10 strands. 10 strands broken into 100 strands. 100 into 1000 1000 into 10,000 10,000 into 100,000 100,000 into 1,000,000 So on & so forth. In a matter of a FEW DAYS
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fireworks_god
Sexy.Butt.McDanger


Registered: 03/12/02
Posts: 24,855
Loc: Pandurn
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Re: Liquid Growth VS spore syringe [Re: agar]
#5418788 - 03/19/06 01:25 PM (17 years, 10 months ago) |
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So one obtains a stir plate, prepares the liquid substrate, inoculates, uses this stir plate to stir the hell out of it, presumably, and it grows like crazy, or something?
 Peace.
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If I should die this very moment I wouldn't fear For I've never known completeness Like being here Wrapped in the warmth of you Loving every breath of you
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