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OfflineBabo911
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Liquid Growth VS spore syringe
    #5418348 - 03/19/06 10:29 AM (17 years, 10 months ago)

I have 2cc of B+ left and I was wondering if i should try to make a liquid culture using karo thanks to this post by road kill or should i just make a spore syringe. I make WBS jars if that plays a difference, I got a pressure cooker but never got a glove box and with the limited space in my apartment i don't plan on getting one till i move into a house. Please let me know.


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Ronhero on the Growery is me


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Invisibleagar
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Re: Liquid Growth VS spore syringe [Re: Babo911]
    #5418389 - 03/19/06 10:48 AM (17 years, 10 months ago)

LC, without question.

You can turn 2 ml into 10,000.


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OfflineBabo911
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Re: Liquid Growth VS spore syringe [Re: Babo911]
    #5418400 - 03/19/06 10:54 AM (17 years, 10 months ago)

that is what i was thinking, i just wanteda a second opinion. And if you know what should i do for a "filtered lid" i was thinking of poking a hole and covering with Tyvek over the lid but under the ring? any suggestions?


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Ronhero on the Growery is me


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Offlinestelthvue
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Re: Liquid Growth VS spore syringe [Re: Babo911]
    #5418405 - 03/19/06 10:55 AM (17 years, 10 months ago)

ive come to learn that 3M micropore tape is amazing for a filter.


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No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.


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OfflineBabo911
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Re: Liquid Growth VS spore syringe [Re: stelthvue]
    #5418410 - 03/19/06 10:57 AM (17 years, 10 months ago)

works for me, now is there any chance i can pick it up at wal-mart, i work there so it be realy convient to get it while i am there


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Ronhero on the Growery is me


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Offlinestelthvue
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Re: Liquid Growth VS spore syringe [Re: stelthvue]
    #5418441 - 03/19/06 11:07 AM (17 years, 10 months ago)

yeah, check the medical section.


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No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.


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Invisibleagar
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Re: Liquid Growth VS spore syringe [Re: Babo911]
    #5418443 - 03/19/06 11:08 AM (17 years, 10 months ago)

Quote:

Babo911 said:
that is what i was thinking, i just wanteda a second opinion. And if you know what should i do for a "filtered lid" i was thinking of poking a hole and covering with Tyvek over the lid but under the ring? any suggestions?





Small bore rubber hose from hardware, or autoparts store.
RTV gasket sealer from autoparts store.


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OfflineBabo911
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Re: Liquid Growth VS spore syringe [Re: Babo911]
    #5418455 - 03/19/06 11:11 AM (17 years, 10 months ago)

one for air exchange the other to suck out the culture i assume? Both stuffed with polly fill. I know this is going to sound stupid but where did you get the plastic top


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Ronhero on the Growery is me


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Offlinestelthvue
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Re: Liquid Growth VS spore syringe [Re: Babo911]
    #5418462 - 03/19/06 11:14 AM (17 years, 10 months ago)

not a stupid question. ive checked a few stores and cant find plastic lids for 1/2 pint kerr jars.


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No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.


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Invisibleagar
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Re: Liquid Growth VS spore syringe [Re: Babo911]
    #5418470 - 03/19/06 11:16 AM (17 years, 10 months ago)

Miracle Whip, and/or Mayo jars @ any grocery store.
(recycling for a differing use is a good thing)


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Invisiblefigment
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Re: Liquid Growth VS spore syringe [Re: stelthvue]
    #5418472 - 03/19/06 11:17 AM (17 years, 10 months ago)

you can get them from Mycosupply.com


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Invisibleagar
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Re: Liquid Growth VS spore syringe [Re: figment]
    #5418478 - 03/19/06 11:20 AM (17 years, 10 months ago)



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Offlinestelthvue
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Re: Liquid Growth VS spore syringe [Re: agar]
    #5418486 - 03/19/06 11:24 AM (17 years, 10 months ago)

sweet agar, thanks. now i wont have to throw out lids because of rust.


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No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.


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OfflineBabo911
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Re: Liquid Growth VS spore syringe [Re: stelthvue]
    #5418514 - 03/19/06 11:35 AM (17 years, 10 months ago)

see lids are cheap for me, it is abot $1.00 for 12, but for LC i found that the lid to the now empty penutbutter jar fits great, i am soo happy


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Ronhero on the Growery is me


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OfflineBabo911
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Re: Liquid Growth VS spore syringe [Re: agar]
    #5418522 - 03/19/06 11:38 AM (17 years, 10 months ago)

Quote:

agar said:
http://www.polsteins.com/prod-0000084.html

http://www.polsteins.com/prod-0000085.html




that is the site i origionally got my jars from


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Ronhero on the Growery is me


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Offlinefireworks_godS
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Re: Liquid Growth VS spore syringe [Re: Babo911]
    #5418549 - 03/19/06 11:46 AM (17 years, 10 months ago)

So, then, one theoretically can take a spore syringe, inoculate some liquid substrate, exponentially increase the amount of solution one can then use to inoculate more jars/casings?

That is to ask, that is the purpose, right? :grin:

:headbang: :headbang: :headbang: :satansmoking:
Peace. :mushroom2:


--------------------
:redpanda:
If I should die this very moment
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For I've never known completeness
Like being here
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:yinyang: :levitate: :earth: :levitate: :yinyang:


Edited by fireworks_god (03/19/06 11:52 AM)


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OfflineTweexican
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Re: Liquid Growth VS spore syringe [Re: fireworks_god]
    #5418557 - 03/19/06 11:48 AM (17 years, 10 months ago)

well THAT, and the promise of faster colonization since the spores are already germinated.

think, g2g in liquid form


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Offlinefireworks_godS
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Re: Liquid Growth VS spore syringe [Re: Tweexican]
    #5418574 - 03/19/06 11:54 AM (17 years, 10 months ago)

I thought of that after I made my post... I assume that it is also quicker/easier for the spores to germinate in the liquid substrate than in, say, a verm/brf substrate?

Definitely something to look forward to trying out. :cool:

:headbang: :headbang: :headbang: :satansmoking:
Peace. :mushroom2:


--------------------
:redpanda:
If I should die this very moment
I wouldn't fear
For I've never known completeness
Like being here
Wrapped in the warmth of you
Loving every breath of you

:heartpump: :bunnyhug: :yinyang:

:yinyang: :levitate: :earth: :levitate: :yinyang:


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Offlineskeletor
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Re: Liquid Growth VS spore syringe [Re: fireworks_god]
    #5418680 - 03/19/06 12:31 PM (17 years, 10 months ago)

its not really exponetial. but if you are growing on a small scale it pretty much is haha


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Invisibleagar
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Re: Liquid Growth VS spore syringe [Re: skeletor]
    #5418749 - 03/19/06 01:02 PM (17 years, 10 months ago)


If you have/use a stir plate.

Growth is EXPONENTIAL.
1 myc strand broken in to 10 strands.
10 strands broken into 100 strands.
100 into 1000
1000 into 10,000
10,000 into 100,000
100,000 into 1,000,000
So on & so forth.
In a matter of a FEW DAYS


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Offlinefireworks_godS
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Re: Liquid Growth VS spore syringe [Re: agar]
    #5418788 - 03/19/06 01:25 PM (17 years, 10 months ago)

So one obtains a stir plate, prepares the liquid substrate, inoculates, uses this stir plate to stir the hell out of it, presumably, and it grows like crazy, or something?

:headbang: :headbang: :headbang: :satansmoking:
Peace. :mushroom2:


--------------------
:redpanda:
If I should die this very moment
I wouldn't fear
For I've never known completeness
Like being here
Wrapped in the warmth of you
Loving every breath of you

:heartpump: :bunnyhug: :yinyang:

:yinyang: :levitate: :earth: :levitate: :yinyang:


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