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Offlinefireworks_godS
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Just How Much Contamination Precautions Are Necessary?
    #5417986 - 03/19/06 05:19 AM (17 years, 10 months ago)

My friend is getting ready to prepare some jars for the first time in a very long time, and he's simply just wondering exactly what lengths are necessary to go to in order to ensure that his jars don't get contaminated.

He's going to clean off all the counters, of course, and spray Lysol over the surfaces is in the kitchen, the jars, the bowls and spoons, himself, the pressure cooker, everything, and then start mixing up the substrate. Provided that the pressure cooker does its job, there shouldn't be too many worries about the jars getting contaminated, right?

That is to say, after they are sterilized, as long as he makes sure the syringe needle is clean, and the jars get put in a tub to incubate that is also lysol'd down, one shouldn't expect to get contaminated?

He's just curious as to whether or not he needs laboratory conditions to ensure his ventures aren't wasted. :grin: Perhaps he's just paranoid... :shocked:

:headbang: :headbang: :headbang: :satansmoking:
Peace. :mushroom2:


--------------------
:redpanda:
If I should die this very moment
I wouldn't fear
For I've never known completeness
Like being here
Wrapped in the warmth of you
Loving every breath of you

:heartpump: :bunnyhug: :yinyang:

:yinyang: :levitate: :earth: :levitate: :yinyang:


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Offlinehawksapprentice
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Re: Just How Much Contamination Precautions Are Necessary? [Re: fireworks_god]
    #5417990 - 03/19/06 05:29 AM (17 years, 10 months ago)

Dont worry about lysoling the jars, your just asking for mutants. If your using a pressure cooker there is also no reason to lysol your pressure cooker as the pressure and heat are going to kill anything that may be in there anyway. Your working area is a good idea though. You can lysol your incubation tub, but I only use H2O2 in my fruiting chamber. I try to use a little lysol as possible, but i just dont like the stuff.


--------------------
"I celebrate the Earth, my home, my mother, my grave, and as long as men are Man they must, if they would preserve the integrated being, do the same---[and preserve]--this rank casual hungry smelly sweaty lusting transitory body, my oozy pulpy liquid-bag-swollen body, bones, blood, hair glands, my bejeweled sex; I love and celebrate it all.  never to let men forget that they are animals as much as gods---that is one thing I shall say."

  Edward Abbey


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Offlinefireworks_godS
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Re: Just How Much Contamination Precautions Are Necessary? [Re: hawksapprentice]
    #5418003 - 03/19/06 06:09 AM (17 years, 10 months ago)

Sounds great. :thumbup:

I guess he was just a little paranoid after reading about the great lengths some will go to sterilize, but perhaps that is when working with casings or something...

:headbang: :headbang: :headbang: :satansmoking:
Peace. :mushroom2:


--------------------
:redpanda:
If I should die this very moment
I wouldn't fear
For I've never known completeness
Like being here
Wrapped in the warmth of you
Loving every breath of you

:heartpump: :bunnyhug: :yinyang:

:yinyang: :levitate: :earth: :levitate: :yinyang:


Edited by fireworks_god (03/19/06 07:47 AM)


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Offlinesivad02487
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Re: Just How Much Contamination Precautions Are Necessary? [Re: fireworks_god]
    #5418011 - 03/19/06 06:17 AM (17 years, 10 months ago)

Well, them more you have EVERYTHING sterilized, the less chance of contamination. I try to do everything possible to sterilize.

Check out this link, it has everything you should know about sterilization
http://www.shroomery.org/index.php/par/23426


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Offlinekungpow
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Re: Just How Much Contamination Precautions Are Necessary? [Re: sivad02487]
    #5418088 - 03/19/06 07:55 AM (17 years, 10 months ago)

Don't worry about sterilizing shit before he pc's the jars. I never clean or anything when I am preparing my jars. You put them in the pc and it sterilizes them. So there is no need for it to be really clean. Now when he inoculates the jars that's a different story. Just make sure he wipes down his work area and spray the air with lysol. He should be all set.

So far I have done over 60 jars with this method and not a single contamination in my jars. I have had one monotub that got contaminated but that's because of low FAE.

~Peace Out~


--------------------
Monotub #3 - check out my best grow to date!!!!



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Offlinefireworks_godS
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Re: Just How Much Contamination Precautions Are Necessary? [Re: kungpow]
    #5418158 - 03/19/06 08:43 AM (17 years, 10 months ago)

I think this was pretty much his attitude before he started getting paranoid. :smirk: :thumbup:

That said, he's going to spray down his work environment beforehand, kitchen counters, sink.. perhaps the dog (:grin:)... Nothing like a clean kitchen, anyway! :wink:

So, as long as we have it narrowed down to keeping everything sterile while he inoculates, wiping down the needle with 70% alocohol should be enough, right? Any concerns as far as the lids go? PF Tek For Simple Minds to start with, tinfoil layers to cover the jar lid, four holes in each lid...

I think everything should be fine. :cool: He ran through the procedure up to inoculation a couple of weeks ago, in order to once again familiarize himself with the process, and it all went smoothly, with the exception of not having enough tin foil to properly cover the lids. He was concerned that water had gotten into the dry layer, and since he wasn't going to inoculate that batch of jars, he opened one up and touched it (the dry layer felt sorta wet). He left the jars for a couple of weeks, took care of them today in order to prepare for an official try at it, and the contents of the jar were untouched by contaminants, with the exception of the one he touched (cobweb type mold sprouting forth from the jar, gotta be more careful :lol:).

Thanks for your help. :thumbup:

:headbang: :headbang: :headbang: :satansmoking:
Peace. :mushroom2:


--------------------
:redpanda:
If I should die this very moment
I wouldn't fear
For I've never known completeness
Like being here
Wrapped in the warmth of you
Loving every breath of you

:heartpump: :bunnyhug: :yinyang:

:yinyang: :levitate: :earth: :levitate: :yinyang:


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Offlinecoda
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Re: Just How Much Contamination Precautions Are Necessary? [Re: fireworks_god]
    #5418388 - 03/19/06 10:48 AM (17 years, 10 months ago)

to be sterile just have a clean work area.  Before you do any work which requires you to be sterile spray lysol in the air, on the counter tops and all surfaces around your grow.  Hit up the sink and its drains, your garbage can, and your floor.  Wipe the surface your working on with alcohol, wash and disenfect your hands, then you're set to go. :smile:


--------------------
To get really high is to forget yourself. And to forget yourself is to see everything else. And to see everything else is to become an understanding molecule in evolution, a conscious tool of the universe. And I think every human being should be a conscious tool of the universe. . . .

-JG

i really am glad you came back to us instead of taking the other path. *hug*

-A_S (RIP your final words to me will never be forgotten)



Don't fuck with the laughing jesus.


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Offlineskeletor
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Re: Just How Much Contamination Precautions Are Necessary? [Re: coda]
    #5418688 - 03/19/06 12:36 PM (17 years, 10 months ago)

use a filter lid and silicone for a self healing inoculation hole. wipe down both with alcohole right before inoculating. now i never bother sanitising my kitchen. Used to get tons of contams when i did... and didn't have these inovations now i havn't had one contam and have fairly lax sterilizations procedures. at least compaired to some.


--------------------
im sorry about your mother. She was a terrible attrative woman.

Get back to nature; hunting burgers and gathering fries.


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InvisibleDIRTYMAN
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Re: Just How Much Contamination Precautions Are Necessary? [Re: coda]
    #5418745 - 03/19/06 01:01 PM (17 years, 10 months ago)

Quote:

coda said:
Before you do any work which requires you to be sterile spray lysol in the air




I don't want to challenge one of the gods here, but I thought it was wiser to spray Oust in the air, Lysol on surfaces. That's what the commercial says anyways  :stoned:


--------------------
I'm racist.                http://k-k-k.com/


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InvisibleSimisu
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Re: Just How Much Contamination Precautions Are Necessary? [Re: DIRTYMAN]
    #5418807 - 03/19/06 01:31 PM (17 years, 10 months ago)

i'd say do as best as you're comfertable with!
i've done a couple of PF tek runs...
this time for example i didn't even bother sterelizing anything for making the spore syrenges... i took a new syrange out of the wrapper sucked some water from a new bottle wiped a small dish with alcohol scraped in the spores with a new scalpel
and that's it... in an open space at my bed room... no spraying anything! worked well enough (no contems at all)

hell i've made an LC and didn't bother making a syrange first... i put a hole in the foil and scraped some spores in there... then i spilled a couple of drops on the foil so they'd drip in with the spores... no contams either...

just do the best you feel comfrtable with imo
of course it's always better to make sure and go over board (won't hurt) but its not 100% neccery...

it also depends what you're after... if you only got one chance with a certine strain or something you might want to make sure you won't fuck up... but if your only worry is getting 3 out of 40 jars contaminated then that's not much of a loss!


--------------------
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