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bluemawkishness
Stranger


Registered: 03/17/06
Posts: 33
Loc: Switzerland
Last seen: 16 years, 3 months
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Re: mycro supply bags VS. jars? [Re: Asc1189]
#5422261 - 03/20/06 12:19 PM (17 years, 10 months ago) |
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my experiences so far:
if you just let them stay around somewhere an wait for colonialization, it will take longer.
if you help a bit, it doesnt take much longer. They need to be remixed/shaken after 3 or 4 days when they started colonization. Additionally I give them fresh oxygen under hepa about every two days thru a small cut with a steril knife where I put some adhesive tape over after. This way they are completely colonizated after approx. 10 days.
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Asc1189
The New Guy

Registered: 11/18/05
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Last seen: 12 years, 10 months
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Additionally I give them fresh oxygen under hepa about every two days thru a small cut with a steril knife where I put some adhesive tape over after. This way they are completely colonizated after approx. 10 days.
Can you please explain this a lil bit more. I understand shaking them, but the air exchange is what I am not understanding.
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bluemawkishness
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Loc: Switzerland
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Re: mycro supply bags VS. jars? [Re: Asc1189]
#5422341 - 03/20/06 12:41 PM (17 years, 10 months ago) |
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I put the bag under the hepa, where the air is steril. Then I make a small knife steril under the alcohol flame and cut a small hole into the bag, wehre I desinfected before with alcohol. Then I press and widen the bag several times, so that the air from inside the bag gets exchanged. After this I put some adhesive tape over the cut to keep the inside of the bag steril, before I take it out of the hepa.
Especially once colonization started, when the spawn needs much oxygen, this greatly accelerates colonization.
I hope you can understand my english and that the idea is clear now.... its not my motherlanguague
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Asc1189
The New Guy

Registered: 11/18/05
Posts: 62
Last seen: 12 years, 10 months
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I think I might try this out. Never have experimented with bags before. How long on PC?
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bluemawkishness
Stranger


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Loc: Switzerland
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Re: mycro supply bags VS. jars? [Re: Asc1189]
#5422491 - 03/20/06 01:24 PM (17 years, 10 months ago) |
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PC, I let them 2 hours and that was so far ok
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BMArts
Stranger
Registered: 01/07/06
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How about reusability? Can you reuse bags like you can jars?
-------------------- Everything I post on this board is pure fiction. Nothing in the post above is real. It is all made up...
May the source be with GNU
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Asc1189
The New Guy

Registered: 11/18/05
Posts: 62
Last seen: 12 years, 10 months
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Re: mycro supply bags VS. jars? [Re: BMArts]
#5422528 - 03/20/06 01:35 PM (17 years, 10 months ago) |
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I wouldnt. Its probably alot easier to clean jars than what it would to a bag. And besides there really cheap. Id just throw em away and grab a new one.
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bluemawkishness
Stranger


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Re: mycro supply bags VS. jars? [Re: BMArts]
#5422539 - 03/20/06 01:38 PM (17 years, 10 months ago) |
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reusability ?
I doubt if this is possible. At least the bags that i use get much crinkly after using once. I thought about reusing as well, but then I never dare it. Maybe there are stronger bags available as well which you can reuse
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Asc1189
The New Guy

Registered: 11/18/05
Posts: 62
Last seen: 12 years, 10 months
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I think reusable and contaimination would get along very well. Dont you?
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BMArts
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Re: mycro supply bags VS. jars? [Re: Asc1189]
#5422551 - 03/20/06 01:42 PM (17 years, 10 months ago) |
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probably... but it is definetly a factor going for jars. They are cheap yes, but still every little bit adds up and having jars that you can use over and over again is definetly an advantage to having to order bags every so often
-------------------- Everything I post on this board is pure fiction. Nothing in the post above is real. It is all made up...
May the source be with GNU
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tiny_rabid_birds
Nocturnal


Registered: 11/08/05
Posts: 15,653
Loc: estados unidos
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Re: mycro supply bags VS. jars? [Re: Asc1189]
#5422560 - 03/20/06 01:44 PM (17 years, 10 months ago) |
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Quote:
Asc1189 said: I think reusable and contaimination would get along very well. Dont you?
The integrity of the bags would be my main concern, not contamination. I mean, you reuse jars over and over again without worrying that they will contaminate simply because they've been used.
Bottom line, if you find bags capable of holding up through multiple runs in the PC, all you have to do is wash them out to reuse them. You sterilize everything all over again, so any contaminants would be killed in the PC anyways.
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bluemawkishness
Stranger


Registered: 03/17/06
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Loc: Switzerland
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Re: mycro supply bags VS. jars? [Re: BMArts]
#5422564 - 03/20/06 01:46 PM (17 years, 10 months ago) |
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there is the risk that in the crinkly surface, the bags might get small holes, maybe very small ones, that you dont even see or feel, but there certainly contaminations can enter...
So i prefer not to reuse, I think its not worth the risk
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tiny_rabid_birds
Nocturnal


Registered: 11/08/05
Posts: 15,653
Loc: estados unidos
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But that's just like I said, the low integrity of the bags. If it develops small holes, then it obviously cannot withstand multiple runs in the PC.
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inoculatedGreif
greif struken


Registered: 03/14/06
Posts: 663
Last seen: 17 years, 5 months
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trd, wht do you use? bags or jars?
-------------------- one branch of man turns away what has made us who we are, the other,excepts it for how its made man. which path do you choose? ------------------------------------- Man takes advantage of who he is,nature is his home, where he evolved from. So why does he turn it down, defy, and mutalate his birth ground? why does he spread disease, murder his native animals,rape and torture his land, and still feel descent of who he is? live a life that is not true? excepts an artificial home?
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tiny_rabid_birds
Nocturnal


Registered: 11/08/05
Posts: 15,653
Loc: estados unidos
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Both. Bought jars in the beginning, but I still have them, so I'll use them when I'm not planning on doing a big project. If I'm going big, I use filter patch bags from themadseason, with a blot of silicone on it to act as a self-healing injector site.
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inoculatedGreif
greif struken


Registered: 03/14/06
Posts: 663
Last seen: 17 years, 5 months
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do they have a web site?
-------------------- one branch of man turns away what has made us who we are, the other,excepts it for how its made man. which path do you choose? ------------------------------------- Man takes advantage of who he is,nature is his home, where he evolved from. So why does he turn it down, defy, and mutalate his birth ground? why does he spread disease, murder his native animals,rape and torture his land, and still feel descent of who he is? live a life that is not true? excepts an artificial home?
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tiny_rabid_birds
Nocturnal


Registered: 11/08/05
Posts: 15,653
Loc: estados unidos
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