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Offlinetengumai
Bioengineeredfor the win
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Registered: 03/08/06
Posts: 32
Last seen: 17 years, 8 months
PCing WBS: How far in advance of inoc?
    #5410437 - 03/16/06 10:06 PM (17 years, 10 months ago)

My first spore syringes should arrive on Monday, and I'm scheduled to fly out of town Monday night for several days, so I'll be on a bit of a schedule.

I'd probably won't have time to PC and cool the WBS (in tyvek/dual-hole/coffee-filter-covered jars) Monday, so I'd like to get them ready for inoculation ahead of time. How many days in advance is too much?

And if I *dont* get my syringes Monday, I won't be able to inoculate until the next Saturday. Does that mean I need to throw out the WBS I prepared in advance, or should it be fine to inoculate even though it's a week old?

Finally, should I refrigerate non-inoculated WBS?

Thanks,
Tengu


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Invisibleagar
old hand
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Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: PCing WBS: How far in advance of inoc? [Re: tengumai]
    #5410477 - 03/16/06 10:19 PM (17 years, 10 months ago)

With that time frame & maybes.
I would prep a tubing filter LC.

Then, if your rigs arrive.
Knock one up.
Then, store it in a warm clean place.
PC your wbs jars, when you get back.

Then after an overnight cooling off.
Use the LC to knock them up. :grin:


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InvisibleMindsEye
AMT Fiend
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Registered: 02/20/06
Posts: 645
Loc: cerebral cortex
Re: PCing WBS: How far in advance of inoc? [Re: agar]
    #5410488 - 03/16/06 10:23 PM (17 years, 10 months ago)

Tube Top Lc's rule


--------------------
Easy Poo Cake Tek


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Invisiblecappa
Nerd
Registered: 02/12/06
Posts: 854
Re: PCing WBS: How far in advance of inoc? [Re: MindsEye]
    #5410855 - 03/17/06 12:06 AM (17 years, 10 months ago)

lol!
Do your damned laundry instead of playing with LC for a change!  :smile:


--------------------
Their are 10 types of people. Those that understand binary, and those who don't.
~Cappa.


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Offlinetengumai
Bioengineeredfor the win
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Registered: 03/08/06
Posts: 32
Last seen: 17 years, 8 months
Re: PCing WBS: How far in advance of inoc? [Re: agar]
    #5411610 - 03/17/06 07:41 AM (17 years, 10 months ago)

Quote:

agar said:
With that time frame & maybes.
I would prep a tubing filter LC.
Then, if your rigs arrive.
Knock one up.





Great idea. I wanted some LC anyway for round 2.

Thanks!
Tengu


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