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Offlinebigsexy
Stranger
Registered: 03/01/06
Posts: 7
Last seen: 17 years, 9 months
Help!!!! 40 jars inoculated, and none showing germination!
    #5401983 - 03/15/06 01:49 AM (17 years, 10 months ago)

It's been a week and a half, and I inoculated some 40 jars and NONE of them are showing any signs of colonization!

I am almost 95% certain that the syringes must have been OK because I inoculated one jar that I ordered with it. Curious though, that one jar took almost 3 weeks to show anything and never colonized except for one little spot.

So basically, I don't know if it's my substrate, or it could be the syringes, I don't know! 40 jars and nothing is showing. I've made cakes once before succesfully, but I don't know what could have gone wrong. Even if the substrate was bad, wouldn't the site where the spores were injected show at least something?


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Offlinexaxphaanes
Mycologist
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Registered: 08/08/05
Posts: 2,988
Last seen: 1 year, 5 months
Re: Help!!!! 40 jars inoculated, and none showing germination! [Re: bigsexy]
    #5401993 - 03/15/06 01:54 AM (17 years, 10 months ago)

pf method? grains ? what....... where did you get the syringe ?


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"Anything i say is fictional"
  what you should look for in manure


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Invisiblecappa
Nerd
Registered: 02/12/06
Posts: 854
Re: Help!!!! 40 jars inoculated, and none showing germination! [Re: xaxphaanes]
    #5402000 - 03/15/06 01:57 AM (17 years, 10 months ago)

You made sure to chant over them right? lol!


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Their are 10 types of people. Those that understand binary, and those who don't.
~Cappa.


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Offlinealsnow469
Stranger

Registered: 10/15/05
Posts: 392
Last seen: 6 months, 13 days
Re: Help!!!! 40 jars inoculated, and none showing germination! [Re: cappa]
    #5402018 - 03/15/06 02:05 AM (17 years, 10 months ago)

well now!!

That certainly sucks! :frown:


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Offlinebigsexy
Stranger
Registered: 03/01/06
Posts: 7
Last seen: 17 years, 9 months
Re: Help!!!! 40 jars inoculated, and none showing germination! [Re: xaxphaanes]
    #5402023 - 03/15/06 02:13 AM (17 years, 10 months ago)

I used the basic original PF Tek from this site, BRF & vermiculite, followed exactly. I got the syringes from a vendor other than listed here because I used them before I joined this board and they did ok before. But now I'm not sure anymore.


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Invisibledoubledutch
Morado PR

Registered: 07/19/05
Posts: 266
Loc: Puerto Rico
Re: Help!!!! 40 jars inoculated, and none showing germination! [Re: bigsexy]
    #5403646 - 03/15/06 02:05 PM (17 years, 10 months ago)

Is there any way that your jars or needles were still hot from sterilization upon inoculation? Did you shake your syringes before inoculation?
banana banana banana banana terracotta banana terracotta terracotta pie.


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"A delightful, wholesome experience for the family." - Desson Thomson, THE WASHINGTON POST

http://www.shroomery.org/forums/showflat...rue#Post5481456

omfg~dutch!


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Offline4skin
Azurescens Pimp
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Registered: 03/11/06
Posts: 46
Last seen: 17 years, 9 months
Re: Help!!!! 40 jars inoculated, and none showing germination! [Re: doubledutch]
    #5403662 - 03/15/06 02:08 PM (17 years, 10 months ago)

I would check temp. When mine are slow it usually because my temp is to low. For cubes i like high 70's to low 80's and they take off like crazy but high 60's to low 70's and it weeks before anything good comes along!


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InvisibleFooMan
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Registered: 02/02/05
Posts: 8,957
Loc: Earth Flag
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Re: Help!!!! 40 jars inoculated, and none showing germination! [Re: bigsexy]
    #5403721 - 03/15/06 02:17 PM (17 years, 10 months ago)

Quote:

bigsexy said:
I got the syringes from a vendor other than listed here because I used them before I joined this board and they did ok before. But now I'm not sure anymore.




I'm thinking Earths Tongue? If so, I made that mistake a long time ago too. 40 jars? :crazy:

Ralph ships fast, get your money order to him QUICK! :grin:


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Quick WBS Prep


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OfflinerDr4g0n
Young Hand
Male User Gallery
Registered: 01/17/06
Posts: 587
Last seen: 17 years, 7 months
Re: Help!!!! 40 jars inoculated, and none showing germination! [Re: FooMan]
    #5403839 - 03/15/06 02:42 PM (17 years, 10 months ago)

when i made my first batch, i did about 3 different methods. that way, if one screwed up, i could hope for the other method to work. dont put all your eggs in one basket i guess...

anyways, either the growth is slow due to environmental condition, the spores you got were bad, or the spores were killed because the substrate was still hot when you inoculated. if you arent seeing anything else growing in there, thats a good sign though.


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i can speel... im just too lazy to sppelcheck.

My first trip (good read) - Speed Leaching Poo! - My Second Trip (with art)


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