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InvisibleLiquidkick
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Registered: 05/03/02
Posts: 2,635
LC senescence?
    #5398409 - 03/14/06 12:00 PM (14 years, 10 months ago)

If i go LC to LC to make more, i am assuming that sooner or later it will senescence? Correct?


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OfflineDelinquentes
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Re: LC senescence? [Re: Liquidkick]
    #5399333 - 03/14/06 04:07 PM (14 years, 10 months ago)

Sooner or later there will be a degredation of the genome. Apart from that, I have read that LC's will fail to take up nutirients if left in the same solution for too long a period of time.


For those without a dictionary:

se?nes?cent ( P ) Pronunciation Key (s-nsnt)
adj.
Growing old; aging.


--------------------------------------------------------------------------------
[Latin senscns, senscent- present participle of senscere, to grow old inchoative of senre, to be old, from senex, sen-, old. See sen- in Indo-European Roots.]
--------------------------------------------------------------------------------
se?nescence n.

[Download Now or Buy the Book]
Source: The American Heritage? Dictionary of the English Language, Fourth Edition
Copyright ? 2000 by Houghton Mifflin Company.
Published by Houghton Mifflin Company. All rights reserved.


se?nes?cence (s-nsns)
n.

The process of growing old; aging.


Source: The American Heritage? Stedman's Medical Dictionary
Copyright ? 2002, 2001, 1995 by Houghton Mifflin Company. Published by Houghton Mifflin Company.


Main Entry: se?nes?cence
Pronunciation: si-'nes-&n(t)s
Function: noun
: the state of being old : the process of becoming old


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Offlinemogur
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Registered: 11/15/05
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Re: LC senescence? [Re: Delinquentes]
    #5399411 - 03/14/06 04:25 PM (14 years, 10 months ago)

Yep, yep, yep. As you move even part of the mycelium from LC to LC, it is aging because it is the same mycelium continuing to grow. The way around it is simple. Put the master culture in a ziplock and stick it in the fridge. Pull it out when you want to spawn another culture. That way, it will always be a second generation growth. When you run out of the master culture, you'll be too old to shroom, anyway.  :headbang: J/k, I mean your friend will be too old.


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OfflinemushboyM
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Re: LC senescence? [Re: mogur]
    #5399520 - 03/14/06 05:05 PM (14 years, 10 months ago)

im confused dude. Put the master culture in the fridge. does the fridge keep it cold which slows growth the same way as it would its age? im really high so maybe i need to read it again in a few hours.

Could you freeze liquid culture? id imagine if you could, it would last a damn long time. the only problem would be keeping it clean.


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OfflineHighGuy
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Registered: 12/08/05
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Re: LC senescence? [Re: mushboy]
    #5399567 - 03/14/06 05:15 PM (14 years, 10 months ago)

I beleive what he is saying is that instead of randomly choosing one of your LCs to inoculate another LC, always use the "master" LC from the fridge. This way instead of getting third, fourth, fifth generation mycelium, you will always be getting second generation myc. Hope I cleared that up?


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Offlinemogur
regnartS

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Re: LC senescence? [Re: HighGuy]
    #5401865 - 03/15/06 03:00 AM (14 years, 10 months ago)

Thanks, HighGuy, exactly right. Subsequent generations lose vigor, so to avoid that, it is better to re-spawn from older generations repeatedly, rather than to let the mycelium age.

Do NOT freeze a culture, the cell walls are ruptured by the freeze/thaw cycle of home freezers and will be destroyed. Labs and commercial houses routinely freeze cultures successfully by either freeze-drying or using liquid nitrogen, but special procedures are followed.

Refrigeration will slow the LC growth, and can be used to greatly extend its life, but home fridges are so full of contams that LCs should be sealed in an airtight container (ziplock or other) for protection.


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