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OfflineSilly_Cyben
not-quite-newbie

Registered: 04/11/05
Posts: 183
Loc: USA
Last seen: 9 months, 1 day
Success making my first spore syringes
    #5399485 - 03/14/06 02:52 PM (17 years, 10 months ago)

I wanted to report on the success I've had using a method I learned here that involves minimum attention to sterile technique. I use one of my 1/2 pint PF jars but seal the four holes with silicone sealer. Then I pressure cook the jar with the lid loose to sterilize it. 20 minutes at 15 psi seems to work fine. After the jar has cooled in the cooker, I screw the lid on tight.

When I get a specimen from which I want to collect spores, I cut off the cap and place it gill-side down in the jar using a pin or straightened paper clip to avoid touching the cap, opening it just long enough to place the cap inside. I leave the cap in 24 hours, then remove it using a wire to avoid touching the inside of the jar. I wear a surgical mask and gloves for this. I leave the cap loose with the jar turned upside down overnight to let the spores dry. Then I screw on the cap and store in a dark, cool place until ready to use.

I sterilized distilled water in 10 cc syringes in the pressure cooker, again 20 minutes at 15 psi. I kept the covered needle point facing upward, as recommended, to keep the water from boiling out. When these are cool, I clean the top of the PF jar with alcohol, and inject the sterile water forcefully onto the spore print and shake vigorously. When all 10 syringes have been injected, I then flame the needles, cooling them quickly in alcohol, and withdraw spore water back into the syringes, shaking the PF jar each time to redistribute spores.

I then injected the spore solution in pint jars of sterilized rye grain, shaking immediately and again after the spores germinated and colonize about 20% of the rye. I got contamination in two jars out of seven that may have occurred during inoculation since the other jars were clean. From one single cap of Penis Envy I was able to make 10 spore syringes. I could have made more if I had more syringes, but my pressure cooker only holds about ten at a time.


Edited by Silly_Cyben (03/19/06 02:24 PM)


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InvisiblemushboyMDiscord
modboy
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Registered: 04/24/05
Posts: 32,274
Loc: where?
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Re: Success making my first spore syringes [Re: Silly_Cyben]
    #5399547 - 03/14/06 03:11 PM (17 years, 10 months ago)

of PE?? damn nice job man, ive heard those are tricky. show us some pics when they fruit!


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Offlinetonyperez420
Shaman Rasta

Registered: 11/03/04
Posts: 1,234
Last seen: 10 years, 11 months
Re: Success making my first spore syringes [Re: mushboy]
    #5399579 - 03/14/06 03:18 PM (17 years, 10 months ago)

yea I am envious.... I am yet to make my own syringe... hopefully in the next month or so when I have a bunch of fruits..

and a PE at that!

Good job and nice tek!


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OfflineBabo911
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Registered: 09/05/05
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Re: Success making my first spore syringes [Re: mushboy]
    #5399594 - 03/14/06 03:22 PM (17 years, 10 months ago)

I may have to use your tek to make my own, i also have yet to make my own. Today I made my first WBS jars and i am hopeing they will work. I have 8cc of B+ left that i am going to knock up 6 jars with and leave 2cc as a safty. I have a pressure cooker but no glove box. So you put 10 cc of water in and then PC with the needle up? Thanks again.


--------------------

Ronhero on the Growery is me


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InvisibleRoadkillM
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Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: Success making my first spore syringes [Re: Silly_Cyben]
    #5399602 - 03/14/06 03:24 PM (17 years, 10 months ago)

nothing personal...

but I hate the method of putting a mushroom cap into a jar and making a spore print.

I just hate scraping spores from the bottom of a jar.

its a pain in the ass!~


tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineBabo911
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Re: Success making my first spore syringes [Re: Roadkill]
    #5399617 - 03/14/06 03:28 PM (17 years, 10 months ago)

what method would you recoment to a guy with a PC and no glove box from after the mushroom unvails to spore syringe


--------------------

Ronhero on the Growery is me


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OfflineSilly_Cyben
not-quite-newbie

Registered: 04/11/05
Posts: 183
Loc: USA
Last seen: 9 months, 1 day
Re: Success making my first spore syringes [Re: Roadkill]
    #5399632 - 03/14/06 03:34 PM (17 years, 10 months ago)

To be honest, Roadkill, I didn't scrape the bottom of the jar. I merely shook it vigorously. The two syringes I've used so far both produced clean cultures, so apparently enough spores get dislodged this way. I can always scrape the bottom for the next batch from the same jar...


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