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Offlinewillmafingerdo
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what do you use to make your LC for pan's??
    #5394491 - 03/13/06 08:07 AM (17 years, 10 months ago)

well i tried making up a LC for my pan cyan using a 4% karo solution w/ no sucess.. just wondering if ppl could post what they've used and how long it took.. everythign ive read said that in 3-5 days your pan LC should be ready to use.. just looking for some input, thanks..

l8r,
will


--------------------
:scaryshroom: :shitstorm: :mattz: :bongload: :gethigh: :scaryshroom:

I am not lost.. i am going there looking for it.. and when i get there and find it, i will know what it is..

"The way to stop violence is not to go out and let your head be beaten in but to say, you want to take my life risk yours!"

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remember opions are like assholes, everyone has one and they all stink.


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OfflineHoss
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Re: what do you use to make your LC for pan's?? [Re: willmafingerdo]
    #5394544 - 03/13/06 08:46 AM (17 years, 10 months ago)

I did te 4% karo and it worked just fine, but it took far longer than 3-5 days before it even germinated. Much longer until it was ready for use.


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InvisibleCaptainKirk

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Re: what do you use to make your LC for pan's?? [Re: Hoss]
    #5394554 - 03/13/06 08:51 AM (17 years, 10 months ago)

i went with Vanilla extract and a shot of karo :wink:  5 mls.of vanilla,1 ml of karo ,200ml's of h2o


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OfflineTheLegend
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Re: what do you use to make your LC for pan's?? [Re: CaptainKirk]
    #5394565 - 03/13/06 08:56 AM (17 years, 10 months ago)

Just use normal LC and give it 3-5 days to see any sign of growth not to completion and then once you see a bit then theres no stopping it from taking over the LC ... then once you inject it to substrate it nearly starts the second it hits the substrate ... i love pans so far :smile: fingers crossed


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Offline_OttO_
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Re: what do you use to make your LC for pan's?? [Re: TheLegend]
    #5394588 - 03/13/06 09:09 AM (17 years, 10 months ago)

Good ole' natural honey... Hasen't failed me yet.


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OfflinerDr4g0n
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Re: what do you use to make your LC for pan's?? [Re: _OttO_]
    #5394617 - 03/13/06 09:22 AM (17 years, 10 months ago)

i made 2 lc, one with honey and water, and one with honey, water, and cooking oil. the one with the cooking oil grew awesome. the one without oil didnt grow at all. i have no idea why...


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OfflineDelinquentes
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Re: what do you use to make your LC for pan's?? [Re: rDr4g0n]
    #5395335 - 03/13/06 02:13 PM (17 years, 10 months ago)

Cooking oil? Interesting. Is there a tek I miss on this? If not, what is the reasoning behind it?

Curious.


--------------------
"The price of meat has just gone up,
and your old lady has just gone down!" ~~ Zappa



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Edited by Delinquentes (03/13/06 02:14 PM)


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OfflineTheLegend
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Re: what do you use to make your LC for pan's?? [Re: Delinquentes]
    #5396097 - 03/13/06 06:12 PM (17 years, 10 months ago)

im sure curiosity was behind it :smile:


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Offlinewillmafingerdo
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Re: what do you use to make your LC for pan's?? [Re: TheLegend]
    #5397120 - 03/13/06 11:00 PM (17 years, 10 months ago)

sweet ty all for the input!!  ill just make up another LC and give it another try.. hope it works out im running out of spore solution :frown:  what temps did everyone incubate at??  once agin, ty for all the input!! hopefully i get some growth this time!!

l8r,
will


--------------------
:scaryshroom: :shitstorm: :mattz: :bongload: :gethigh: :scaryshroom:

I am not lost.. i am going there looking for it.. and when i get there and find it, i will know what it is..

"The way to stop violence is not to go out and let your head be beaten in but to say, you want to take my life risk yours!"

"are you sure u want to eat all them shrooms??"  "yes i want to hear what my mind has to say..."

remember opions are like assholes, everyone has one and they all stink.


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OfflineBlue Helix
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Re: what do you use to make your LC for pan's?? [Re: willmafingerdo]
    #5397486 - 03/14/06 12:55 AM (17 years, 10 months ago)

I use 10 grams of dextrose and 10 grams of light malt powder per 500 ml (about a pint) of water. I use a stir magnet and constantly stir the mix. Pan cyans in this mix RIP through LCs like no one's business. Using about 2 ml of starter liquid culture, I get completion in no more than 3 days. Using about 1 or 2 ml of spore solution, it takes about 5 to 7 days depending on how old the spores were and the strain. These are no exaggerations!

Here is an viet pan cyan LC that was started by about 8 ml of LC in 48 hours (viets are super fast):


Here is a ausi pan cyan LC that was started from 1ml of spores after about 7 days:


Both of those cultures were proven good by subsequent fruitings.


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Offline_OttO_
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Re: what do you use to make your LC for pan's?? [Re: Delinquentes]
    #5398096 - 03/14/06 07:58 AM (17 years, 10 months ago)

Quote:

Delinquentes said:
Cooking oil? Interesting. Is there a tek I miss on this? If not, what is the reasoning behind it?

Curious.




Try doing a search for 'flocculation' with google for answers.

Oil encourages 'flocculation' of mycelial strands in a substrate like h/poo, so it stands to reason it would work well in a LC as too.


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OfflineBlue Helix
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Re: what do you use to make your LC for pan's?? [Re: _OttO_]
    #5398347 - 03/14/06 09:48 AM (17 years, 10 months ago)

Cooking oil work fine as a very addition to a bulk substrate (tablespoon maybe in a gallon of substrate), but it should not be added to a LC. I am not sure how that worked, but as oil floats, it would effectively seal the surface layer from breathing and suffocate the LC unless you happen to be using a stir plate. Do not use oil in your LC, not even a drop.

Incidentally, if you are looking for flocculation in a bulk substrate, a better addition is gypsum. Cooking oil serves primarily as a nutritional amendment and less effectively as a flocculation agent.


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Offlinescatmanrav
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Re: what do you use to make your LC for pan's?? [Re: Blue Helix]
    #5398402 - 03/14/06 09:58 AM (17 years, 10 months ago)

I was wondering about adding gypsum and oyster shell to bulk. Wondering if it would be a waste? Maybe it would be a waste of oyster shell (have to order online to get the good stuff) but gypsum I got lots of and its easy to find..maybe I will try some if you think it helps.

I do add oil to my bulk everytime (about 1 oz per big batch).


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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OfflineBlue Helix
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Re: what do you use to make your LC for pan's?? [Re: scatmanrav]
    #5398436 - 03/14/06 10:06 AM (17 years, 10 months ago)

scatmanrav, I don't think oyster shell is a good idea. I tried something like that and noticed a reduction of mycelium vigor. Maybe it wasn't the oyster shell, though.

If you are dealing with a straw-based bulk substrate, I think gypsum is very good idea since it'll help the nutritional ooze of the grains, flour, and oils stick to the straw pieces, including their hollow interiors, better. That helps the straw really serve as a better nutritional conduit for the mycelium growth. For normal manure/grain substrates, I would not bother with gypsum.


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