Psi. zapotecorum succesful cultivation (pics) - Advanced Mycology

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Elektrolurch
enthusiast

Registered: 05/01/00
Posts: 307
Loc: Germany
Last seen: 19 years, 3 months

Psi. zapotecorum succesful cultivation (pics)

3
#539238 - 02/03/02 12:00 PM (23 years, 4 months ago)

Hi,

I was waiting since some time to post this results, because I wanted to go for sure. Now after BIO posted again about his Psi. zapotecurom pinning again, I thought the time would be suitable.

I managed to find some specimens of Psi. zapotecorum the last summer in the Zapotec sierra in Oaxaca (see Photo underneath).

I managed to gain pure cultres, by cloning of the above carpophores, and won also cultures from spores (and from other specimens not shown).

I inoculated sterilized straw within a jar with a clone-culture (from the left carpophore in the above picture) . After some time of incubation (room temperature 20 C) the micellium colonized the straw, which was used to spawn bigger amounts of pasteurized straw.

I filled a small tray with colonized straw. I let it recover for some days and cased it with a 3 cm thick soil/loam mixture, which was pasteurized and incubated for some weeks before application.
After a week of incubation the tray was putten into a fruiting chamber at 20 C with a 15 W light. The tray was covered with a thin foil to have a high humidity within it.

After some weeks it started to pin. When the fruitbodies achieved a size that was too big for the foil over the tray, I used a plastic bag (this within a terrarium that has already above 90 % humdity!) . Within two weeks one of the fruitbodies reached maturity and is sporulating, other are commeing. See the pictures above for a time series (about 2 weeks, taken about every two days, the last three ones are from the same day).

The interesting thing is that the cap has an abnormal form. After some days of research, and specially because the culture is a clone of the above specimen, I'm sure they are zapotecorum. Probably the conditions in the terrarium changed their appearance.

A very good sign that they are Psi. zapotecorum is the stem that is "floccose above to strongly scabrous-strigose at the base" (Guzman). Also the Lamellae (Gills) are specific, namely "sinuate or adnate". The fruitbodies show also a very soon bluing reaction when injured or touched, which tends to have "blackish violet tones"

Guzman also writes about the pileus (cap): "from conic or convex when young to subumbonate and papillate or convex to campanulate to convuluted or subconcave when old" The young specimens have the normal form, but the older ones are slightly more than subconcave. The first flush is always kind of different looking than the next ones (at least with cubensis, semperviva etc.).

The colour of the cap is somewhat light, but is probably due to the strong light. The specimens we found showed that the ones in the darker places had darker caps (brown) and the ones growing in places with light had more a straw-like colour.

I'm still not completely sure shich mushroom it is. The next possible candidate would be Psi. argentipes (just kidding, argentipes is only known from Japan :wink:

, but the description is very similar to the above fruitbodies).

Until now Psi. zapotecorum fits the best, I'm willing to know what you think about it. Thanx for your time,

Elektrolurch

--------------------
"For all the time spent in that room
The doll's house, darkness, old perfume
And fairy stories held me high on
Clouds of sunlight floating by.", Pink Floyd '67

Extras:

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