Home | Community | Message Board

Magic-Mushrooms-Shop.com
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Substrate Bags   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Injection Grain Bag

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineTheLegend
Stranger
Registered: 05/23/05
Posts: 217
Last seen: 14 years, 6 days
Loads of first time agar contam haha done in my glovebox
    #5391807 - 03/12/06 12:29 PM (17 years, 10 months ago)

Firstly my glovebox is good :smile: but i think the detol (european lysol) spray is a bit harsh haha The pics are while i was still making it, i siliconed some of the plastic i cut for the arms over the breath holes as this was my first Fruiting chamber... and i clean it with detol before every use.



And with most/all my plastic petris i got contam bad! my glass ones arnt bad considering some are outdoor prints im using on them. Azur etc

This is the main contam(the black spot is a clump of spores
Contam 1




underneat


And more from a different petri



Then this is the next contam
contam 2



yellow dots , didnt get many of these this time :smile: first open aired attempt i got loads of them :smile:

Contam 3 probably from azur print cause its right beside or ontop of spores (not worried about this once off thing)



contam4 (again its a once off so dont care)




And then pics of good and ok ones that i can isolate from









Its just wierd that this type of contam


The mycelium still grows on it like its not contamed at all... doesnt stall it at all.


So theres a massive collection of fucked petris hahaha all from one session in the glove box ... i finally found some isopro alcohol so do you think it would be better to whipe the glovebox down with this and spray the alcohol instead of the detol(lysol)?


Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery

Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
Trusted Cultivator
OG Cultivator
Re: Loads of first time agar contam haha done in my glovebox [Re: TheLegend]
    #5391997 - 03/12/06 01:42 PM (17 years, 10 months ago)

Nope. A glovebox need not be sterile. That's bacteria growing in those petri dishes. Be sure to never leave the lid off a petri dish for more than five seconds. That's long enough to lift the lid, make the pour, then replace the lid. Be sure to wipe down the gloves with alcohol before use. You also need to wrap the dishes with parafilm as soon as they cool. With that much bacteria in your dishes, I'd say your bottle of agar got contaminated prior to your pour. I use a whiskey bottle for agar, with a synthetic filter disk cut to fit inside the lid of the bottle, and a 1/4" hole drilled in the lid. This prevents the agar from getting contaminated as it cools from the PC cycle.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


Extras: Filter Print Post Top
OfflineTheLegend
Stranger
Registered: 05/23/05
Posts: 217
Last seen: 14 years, 6 days
Re: Loads of first time agar contam haha done in my glovebox [Re: RogerRabbit]
    #5392131 - 03/12/06 02:36 PM (17 years, 10 months ago)

The mad thing is i poured all the agar from two seperate jars .... then i done the parafilm as they cool ... then i even incubated them for about a week to make sure my tek was right and everything was contam free, and they were perfect.

But with the plastic ones i had a good 3-4ml of condinsation running around so in the glovebox before each inoculation i helt it on its side and over a clean tissue i opened the lid a tiny amount so the water would run out onto the tissue and id say at that point all the bacteria rushed in replacing the space the water took up.

Im DREADING doing an isolation :smile: if i cant get a print to agar without contam im going to be fucked opening a petri cutting a bit out and then opening another jar and putting it in .... all in 5-6 seconds and without causing to much air movement haha

Im thinking of putting an airpump inside a lunchbox stuffed with pollyfil and only one big hole at the top with tyvek over the hole and then the air to go from the tubing to another jar stuffed with pollyfill and then from there go through the glovebox(very tight holes already made just covered) and then into a glass of water and h202 with a bubbler on the end .... and before each use id open the middle jar and spray the polyfil with either lysol or alcohol



The idea would be any small gap like around the lid would have air being pushed out of it instead of any air being allowed in and also this would lessen the chance of me blowing myself up spraying flamable stuff inside a sealed box and then using a lighter :smile: even after 20 mins sometimes i get a big flame :smile:


Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery

Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
Trusted Cultivator
OG Cultivator
Re: Loads of first time agar contam haha done in my glovebox [Re: TheLegend]
    #5392796 - 03/12/06 05:56 PM (17 years, 10 months ago)

Still air is what you want for a glovebox. I have nearly 100% success with my glovebox doing agar, so you can too. Brush up on sterile procedures and read all the teks. Oh, and if you'll keep your petri dishes stacked vertically, they won't develop nearly as much condensation.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


Extras: Filter Print Post Top
OfflineHotnuts
old hand
 User Gallery

Registered: 02/26/05
Posts: 3,436
Loc: Wild Blue Yawnder
Last seen: 25 days, 16 hours
Re: Loads of first time agar contam haha done in my glovebox [Re: RogerRabbit]
    #5398785 - 03/14/06 11:42 AM (17 years, 10 months ago)

Also, with plastic dishes it's a good idea to wipe them out well with 3% H202 prior to pouring your agar. I was having so so results when wiping them out with alcohol first. Haven't had 1 problem since using the peroxide.

Just make sure to use the peroxide the night before you plan to pour your dishes. Don't leave beads of it behind in the lids and dishes after cleaning. This allows it to dry up before the pour.


Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery

Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
Trusted Cultivator
OG Cultivator
Re: Loads of first time agar contam haha done in my glovebox [Re: Hotnuts]
    #5399749 - 03/14/06 04:12 PM (17 years, 10 months ago)

Plastic petri dishes come wrapped in a plastic tube and are sterile when you remove them. Don't wipe with anything, and don't remove from the package until you're ready to pour. They cost less than a dime apiece and are not intended to be used more than once.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


Extras: Filter Print Post Top
OfflineHotnuts
old hand
 User Gallery

Registered: 02/26/05
Posts: 3,436
Loc: Wild Blue Yawnder
Last seen: 25 days, 16 hours
Re: Loads of first time agar contam haha done in my glovebox [Re: RogerRabbit]
    #5399873 - 03/14/06 04:38 PM (17 years, 10 months ago)

I was obviously talking about reused dishes.


Edited by hotnutz (03/14/06 05:23 PM)


Extras: Filter Print Post Top
OfflineHotnuts
old hand
 User Gallery

Registered: 02/26/05
Posts: 3,436
Loc: Wild Blue Yawnder
Last seen: 25 days, 16 hours
Re: Loads of first time agar contam haha done in my glovebox [Re: Hotnuts]
    #5400116 - 03/14/06 05:25 PM (17 years, 10 months ago)

Is this your first time with agar, or your fist time with contaminated agar?


Extras: Filter Print Post Top
OfflineTheLegend
Stranger
Registered: 05/23/05
Posts: 217
Last seen: 14 years, 6 days
Re: Loads of first time agar contam haha done in my glovebox [Re: Hotnuts]
    #5402187 - 03/15/06 06:00 AM (17 years, 10 months ago)

thats the thing .. i did have all my agar ready and then opened the pack gently and poured them in but obviously they wernt as sterile in the pack as i thought ... but the 5 glass ones i sterilized and ive just ordered 10 more glass ones and working out about 2euro per dish so once i get them i can forget about plastic ones :smile:

Its basically my first time working with agar at all ... but im sure ill get it next time ill just work slower so less air moves and ill whipe the box with alcohol rather then the lysol stuff


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Substrate Bags   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Injection Grain Bag


Similar ThreadsPosterViewsRepliesLast post
* Question about agar contam *pic* llamaboy 3,776 11 09/26/17 11:23 AM
by The Mycologist
* Bacterial Contam in MEA upupup 2,712 7 11/23/02 11:38 AM
by Seuss
* Contam free MEA upupup 1,537 3 12/12/02 12:28 PM
by upupup
* Give me some tips to fight contams. tekramrepus 2,499 9 02/24/10 04:49 AM
by kmark
* contams---what now? ryacentauri 714 1 12/08/02 12:19 AM
by
* Post deleted by Moe Howard vampirism 1,427 8 11/26/02 04:35 PM
by fredthetree
* agar problems dog 2,582 8 01/16/03 09:37 AM
by MAIA
* ever order contamed syringes? Shaw 1,081 7 12/28/03 12:50 AM
by catalyst777

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
2,812 topic views. 13 members, 153 guests and 44 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.029 seconds spending 0.009 seconds on 14 queries.