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OfflineNewbieS
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Storing an LC needle.
    #5390774 - 03/12/06 12:25 AM (17 years, 10 months ago)

I want to get a litte tupperware container to hold all my sterilized syringes and luquid culture jars. Once I boil my empty syringe for 10 minutes or so, flame the needle, and fill it up with mycelium, can I keep it in the fridge (tupperware) for storage and have it remain sterile? What's the most economical way to ensure streility of a liquid culture syringe?


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OfflineDelinquentes
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Re: Storing an LC needle. [Re: Newbie]
    #5390810 - 03/12/06 12:57 AM (17 years, 10 months ago)

I think you can store them in the fridge just fine. I plan on it.


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OfflineNewbieS
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Re: Storing an LC needle. [Re: Delinquentes]
    #5390896 - 03/12/06 02:00 AM (17 years, 10 months ago)

Would this work as procedure for properly making an LC syringe ?

>Dismantle syringe
>Put in pot of boiling water
>Boil for 10-15 minutes
>Use tongues to lift the pieces out onto a sterile surface
>Wearing gloves, assemble the needle, put in plastic bag, and cool in fridge.
>Suck it full of LC, give it a shake...and refrigerate until use?

...Or should I just keep the syringe sterile and suck up the LC when it's time for inoculation?


Edited by Newbie (03/12/06 02:02 AM)


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Offlineskeletor
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Re: Storing an LC needle. [Re: Newbie]
    #5390997 - 03/12/06 02:58 AM (17 years, 10 months ago)

it would except you are assembling it in the open, dirty air. boil it with water inside that way you never have to expose the inside. anyways theres really no point in the syringe being sterile untill you use it. just suck up some boiling water and quirt out into sink and repeat a few times. cleans the bugger out nicely then wipe down the needle with alcohole and suck up LC.


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