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Offlinexhooliganx
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inoculating through synthetic filtration discs
    #5389745 - 03/11/06 04:47 PM (17 years, 10 months ago)

if I sterizile a jar of wbs and use a synthetic filtration disc for a cap when I'm going to inoculate the wbs do I just sterilize the needle of a spore syringe and push it through the disc and inject my spores or LC? If so should I do this in a glove box? Do I need to wipe down the disc with alcohol?


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Edited by xhooliganx (02/21/07 01:03 AM)


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Offlineshroomsynergy
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Re: inoculating through synthetic filtration discs [Re: xhooliganx]
    #5389752 - 03/11/06 04:50 PM (17 years, 10 months ago)

a filter disc will retain hole shape from the needle if inoculated thru there, so that would defeat the purpose i would think. put a bead of silicon over a hole to create a healing injection site.


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Offlinetonyperez420
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Re: inoculating through synthetic filtration discs [Re: xhooliganx]
    #5389772 - 03/11/06 04:56 PM (17 years, 10 months ago)

lightly mist your GB with 1:10 bleach water

then what I do is I have a wet rag or strong paper towel in there
throw your jar and needle in also.... wait about 10 mins until the bleach water settles... and also throw in a cotton damped with rubbing alcohol

lightly wipe down the jar with the rag... make sure its not dripping wet!

then inject the spores right through the filter disc is what I do (wipe it down with cotton and rubbing alcohol) (if you think your GB is sterile enough then you can open the jar but I would not suggest it)

thats what I do when I use a GB.... you will prob get other answers from other peeps

GL


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Offlinetonyperez420
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Re: inoculating through synthetic filtration discs [Re: tonyperez420]
    #5389775 - 03/11/06 04:57 PM (17 years, 10 months ago)

yea shroomsynergy has a point...

after the GB oust the room and put it in a sterile bin so no contams get through those wholes


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Offlinexhooliganx
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Re: inoculating through synthetic filtration discs [Re: tonyperez420]
    #5389787 - 03/11/06 05:03 PM (17 years, 10 months ago)

will the silicone self heal? would it be best to prepare the filters with silicone injection spots even before I put them on the jars and sterilize?


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Edited by xhooliganx (02/21/07 12:59 AM)


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OfflineRogerRabbitM
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Re: inoculating through synthetic filtration discs [Re: tonyperez420]
    #5389788 - 03/11/06 05:03 PM (17 years, 10 months ago)

Do NOT inject through a filter disk. If you use synthetic filter disks, you'll need to lift the filter up to squirt under it. Do this in a glovebox, or in front of a flow hood.
RR


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Offlinexhooliganx
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Re: inoculating through synthetic filtration discs [Re: xhooliganx]
    #5389791 - 03/11/06 05:04 PM (17 years, 10 months ago)

one other quick Q. How much headspace should I have between the wbs and the filter?


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Edited by xhooliganx (02/21/07 12:59 AM)


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