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Offlinekbarn
Stranger
Registered: 03/04/06
Posts: 20
Last seen: 17 years, 10 months
Purple color in jars = contam?
    #5389580 - 03/11/06 03:54 PM (17 years, 10 months ago)

I have some strange purple colored rye grains in three of my four jars I recently incubated.  They are not fully colonized (only about 60%) but I am assuming this crap is going to just grow more and more...anyone else had this contamination problem?  Know what it is or caused by?  I am starting to get annoyed with lyvek...anyone else have problems with that?  I don't know how I could be more sterile (gloves, oust, ionizer, clean clothes/hands/arms, heat and alcohol needle in between each one) and I am just wondering how I could go about getting less contams.  Right now I am at around 65% contamination rate... :frown: :frown:


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Invisibleohmatic
searcher
 User Gallery

Registered: 02/28/04
Posts: 6,742
Loc: europe
Trusted Cultivator
Re: Purple color in jars = contam? [Re: kbarn]
    #5389609 - 03/11/06 04:02 PM (17 years, 10 months ago)

there is a very simple rule of thumb in this hobby:

everything thats not white is not good

that should explain what to do next (trashem, start over)
hit the contam forum and read the faq in there, very good.
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 11 years, 26 days
Trusted Cultivator
Re: Purple color in jars = contam? [Re: ohmatic]
    #5389964 - 03/11/06 06:11 PM (17 years, 10 months ago)

Purple definatly bad. Trash time.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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Invisibleremediator
IllustriousPotentato
Male

Registered: 02/23/06
Posts: 137
Loc: NOLA ATM
Re: Purple color in jars = contam? [Re: scatmanrav]
    #5390335 - 03/11/06 08:41 PM (17 years, 10 months ago)

hey wait!!!!!!!!!!

Are you using POST OFFICE tyvek?

DOES IT BY CHANCE HAVE RED AND BLUE INK?

red+blue= PURPLE.
I've had red, blue and purple stained grains from tyvek before.

Are you getting any smells?

Do you see powder deposits of purple, or just a stained appearance to the grains?

pics?

(never seen a purple contam. purple mushrooms in the forest, purple-black spores, butnever a purple contam. Anything's possible.

check out yer tyvek first. It really does leak dye onto your mycelium...
peace and good luck
/9and no, i have great luck with tyvek. always great luck. very reliable stuff.


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Invisiblebackupwards
peon

Registered: 04/02/05
Posts: 3,022
Loc: somewhere else
Re: Purple color in jars = contam? [Re: remediator]
    #5390464 - 03/11/06 09:37 PM (17 years, 10 months ago)

no no no no no, its not from the tyvek....i can't believe you would even suggest that or the smelling of possible contams....as already said if it's not white then its not right....

does it look like this:



:peace:


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Offlinekbarn
Stranger
Registered: 03/04/06
Posts: 20
Last seen: 17 years, 10 months
Re: Purple color in jars = contam? [Re: remediator]
    #5390824 - 03/12/06 01:10 AM (17 years, 10 months ago)

pictures:



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Invisibleremediator
IllustriousPotentato
Male

Registered: 02/23/06
Posts: 137
Loc: NOLA ATM
Re: Purple color in jars = contam? [Re: kbarn]
    #5390880 - 03/12/06 01:48 AM (17 years, 10 months ago)

that's terrible....
:puke:
now i know, an knowing is half a sandwhich and a bowl of soup for only 6.99....
sorry mate.

how long are ya pc'ing?


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Offline_Lucid_
journeyman
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Registered: 05/29/02
Posts: 154
Loc: no-mans land
Last seen: 12 years, 2 months
Re: Purple color in jars = contam? [Re: remediator]
    #5390894 - 03/12/06 02:00 AM (17 years, 10 months ago)

> I don't know how I could be more sterile (gloves, oust, ionizer, clean >clothes/hands/arms, heat and alcohol needle in between each one)

Well... i didnt see flow hood or glovebox mentioned in there, so i know how you could be more sterile. I'll give you two guesses. :P
seriously, a simple glove box is really easy to make, even if your not bothering with a possitive pressure, and just making a static one, spraying lysol all around will help you out considerably. Since i made one i havnt had a single jar contam, 100% success rate. It will definitly be well worth your time to construct one, and searching around, there are a lot of teks and examples around on how to build them.


--------------------
The believer is happy, the doubter is wise
- Hungarian proverb


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Offlinekbarn
Stranger
Registered: 03/04/06
Posts: 20
Last seen: 17 years, 10 months
Re: Purple color in jars = contam? [Re: remediator]
    #5390897 - 03/12/06 02:00 AM (17 years, 10 months ago)

PC = 1h30m

is it because of my tyvek or bandaids? i am going to get micropore tape per request instead of the bandaids.


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InvisibleMindsEye
AMT Fiend
Male

Registered: 02/20/06
Posts: 645
Loc: cerebral cortex
Re: Purple color in jars = contam? [Re: kbarn]
    #5391108 - 03/12/06 04:53 AM (17 years, 10 months ago)

I allways have contam issues with rye, thats why I switched to wbs, its easier to find and cheaper too...


--------------------
Easy Poo Cake Tek


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Invisibleremediator
IllustriousPotentato
Male

Registered: 02/23/06
Posts: 137
Loc: NOLA ATM
Re: Purple color in jars = contam? [Re: MindsEye]
    #5391591 - 03/12/06 11:05 AM (17 years, 10 months ago)

Yeah, i never really liked those berries myself.
Nor wbs, too many sunflower seeds.
But all that trouble you went through... then Band-aids?
I mean, i guess so... but that 3m micropore tape is worth it's weight in weed.
And squares of tyvek that cover the entire jar lid with the band and drilled lid in place have never done me wrong either...


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Offlinekbarn
Stranger
Registered: 03/04/06
Posts: 20
Last seen: 17 years, 10 months
Re: Purple color in jars = contam? [Re: remediator]
    #5391683 - 03/12/06 11:49 AM (17 years, 10 months ago)

is rye really that much more susceptible to contamination than wbs? if you don't like rye or wbs then what do you usually grow on remediator? i will definitly switch to micropore tape instead of band-aids.


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Invisibleremediator
IllustriousPotentato
Male

Registered: 02/23/06
Posts: 137
Loc: NOLA ATM
Re: Purple color in jars = contam? [Re: kbarn]
    #5391944 - 03/12/06 01:21 PM (17 years, 10 months ago)

Well.
It just starts to get personal at the point when you specifically state a preference..
But this is me... hold on, lemme get a camera...
50# bag...  Level Pint...  Eyeballin'  Ready...
Pouring...  Perfect!  Tyvek  Ready for PC.

Then PC for an hour and a half or so, then shake as soon as you can handle, to prevent clumping of starches...
Works for me.  Thousands of jars like this :wink:
Keep trying.
use what you've got and be thorough, or seek alternative methods.  Almost any method can be successful, but each has it's own quirks...
good luck!


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Offlinemikeownow
Humungus fungus

Registered: 09/01/05
Posts: 2,856
Loc: WA,USA
Last seen: 17 years, 3 months
Re: Purple color in jars = contam? [Re: remediator]
    #5391968 - 03/12/06 01:33 PM (17 years, 10 months ago)

Contam in your jars wtf? I am the least of sterile and I am impatient. I soak for 20 hours instead of 24 I do not strain and rince properly I pc for only 45 minutes I inoculate without flaming my needle and just rubbing some alchol on it. I do it in a dirty kitchen in a trailor and all my jars are at 90% with NO contams. You must have skipped soaking maybe or simmering?


--------------------
No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.


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OfflineOatman2000
-=Outa Space=-
Male User Gallery

Folding@home Statistics
Registered: 05/10/05
Posts: 2,877
Loc: Planetary Nebula
Last seen: 1 year, 4 months
Trusted Cultivator
Re: Purple color in jars = contam? [Re: mikeownow]
    #5395632 - 03/13/06 03:34 PM (17 years, 10 months ago)

too long of simmer.... starches, leading to a bacterial infection.

when the jar is not colonizing on the bottom... there's something wrong. i bet those jars smell like rotten apples don't they?



--------------------
Spawning to COIR
:thumbup:  My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION
----------------------------

4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine


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Offlinekbarn
Stranger
Registered: 03/04/06
Posts: 20
Last seen: 17 years, 10 months
Re: Purple color in jars = contam? [Re: Oatman2000]
    #5396964 - 03/13/06 10:16 PM (17 years, 10 months ago)

i heard that there might be too much moisture...thanks for the advice


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