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Invisiblemungojerry
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Is this good for 2 days?
    #5386459 - 03/10/06 04:11 PM (17 years, 10 months ago)

Liquid Honey Culture
2 days ago......


Today......

Mainly just cloudy no mycelium strands
Does this look OK?


Edited by mungojerry (03/10/06 04:13 PM)


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OfflineWronguy
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Re: Is this good for 2 days? [Re: mungojerry]
    #5386490 - 03/10/06 04:14 PM (17 years, 10 months ago)

The pictures are too cloudy for an accurate assessment. As long as the mycelium, or cloud, is white you're fine. What did you inoculate the LC with? If you inoculated with spores you may have a contaminate. Spores take 3-5 days for germination. It's pretty rare to see that kind of growth in 2 days without using another liquid culture as your inoculate.

That cloud should have structure to it, not be cloudy throughout. In other words, you should see a small cloud inside the medium.


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Invisiblemungojerry
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Re: Is this good for 2 days? [Re: Wronguy]
    #5386506 - 03/10/06 04:17 PM (17 years, 10 months ago)

i inoculated it with a homemade dark spore syringe


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OfflineWronguy
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Re: Is this good for 2 days? [Re: mungojerry]
    #5386521 - 03/10/06 04:20 PM (17 years, 10 months ago)

If the entire jar is cloudy and smells of fermentation, it may be contaminated. Discoloration, fermentation, and structure that does not appear white in color, are clear signs of contamination in liquid cultures.


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Invisibleagar
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Re: Is this good for 2 days? [Re: mungojerry]
    #5386620 - 03/10/06 04:40 PM (17 years, 10 months ago)

If all the solution is cloudy.
That is NOT a good sign.

Once the spores germinate.
Then you should see small individual myc clouds.
Like the jar - above.


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