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stelthvue
is frightened ofpoo
Registered: 01/20/06
Posts: 272
Last seen: 17 years, 9 months
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What am i doing wrong?
#5385575 - 03/10/06 01:18 PM (17 years, 10 months ago) |
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So i followed the PF tek down to last minute detail. I PCed my jars at 16 psi for 60 minutes. I inoculated last saturday with B+. It been 6 days and out of 11 jars, only 2 show any kind of mycellial growth. I opened up a few jars and saw quite a bit of jar lids with condensation on the top of the jars on the inside. But the two jars with mycellial growth also have this. The jars have been incubating at a steady 83 degrees +/- 1 degree. Is this me being impatient or should i have seen some kind of growth out of all of them so far? The only thing i did do wrong was PC my jars a week before i inoculated them because my spores were delayed. But, i read up and found out the jars would be ok if i left them sealed in the PC like i did. Any ideas?
-------------------- No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.
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tiny_rabid_birds
Nocturnal


Registered: 11/08/05
Posts: 15,653
Loc: estados unidos
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Re: What am i doing wrong? [Re: stelthvue]
#5385586 - 03/10/06 01:22 PM (17 years, 10 months ago) |
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Okay, for one, don't friggin open the jars!! You just exposed your uncolonized substrate to the contaminations you were trying to keep out by sterilizing.
Two, cool your friggin jets holmes. It can take a while for spores to germinate and mycelium to become visible.
There's nothing wrong w/ PCing a week ahead of time.
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stelthvue
is frightened ofpoo
Registered: 01/20/06
Posts: 272
Last seen: 17 years, 9 months
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i know i made a no no. i just cant understand why two jars would colonize super quick and the rest wouldnt.
-------------------- No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.
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musher_420
Stranger

Registered: 08/01/05
Posts: 2,691
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Re: What am i doing wrong? [Re: stelthvue]
#5385601 - 03/10/06 01:25 PM (17 years, 10 months ago) |
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Yes your being impatient. You shouldn't have opened up those jars you just GREATLY increased your chance of contams. Hopefully it was in a really clean enviroment, hopefully the dry verm caught any meanies.
Did you remember to shake your syringe?
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: What am i doing wrong? [Re: stelthvue]
#5385602 - 03/10/06 01:26 PM (17 years, 10 months ago) |
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More spores in them, that germinated.
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tiny_rabid_birds
Nocturnal


Registered: 11/08/05
Posts: 15,653
Loc: estados unidos
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Re: What am i doing wrong? [Re: stelthvue]
#5385603 - 03/10/06 01:26 PM (17 years, 10 months ago) |
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Well that's what you can expect from a multispore inoculation (which is any inoculation using spores as opposed to LC or spawn).
In multispore inoculation you've gotta wait for the spores to germinate. Some will hook up faster than others. And often times, unless the spore solution has been ultrasonicated, you'll have uneven distribution of spores.
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stelthvue
is frightened ofpoo
Registered: 01/20/06
Posts: 272
Last seen: 17 years, 9 months
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ok. thanks for the reassurance guys.
-------------------- No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.
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