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InvisibleJoshua
Holoman
Male

Registered: 10/27/98
Posts: 5,398
Loc: The Matrix
Psilocybe cubensis "Puerto Ricans" on rye/compost
    #536536 - 01/31/02 01:26 AM (22 years, 2 months ago)

Here's a new PR start. Check out the mycelium poking through the holes in the top of the next two pics. I got this from Azurepower. He can't remember if there was a verm layer or not. In the next pic you'll see nothing but mycelium in the top of the jar. He says the strain has only been cultivated once since being picked from the wild. I will be posting updates periodically.







The last pic shows the breaking up of the cake. I then covered the tray w/ foil. I will let the mycelium grow back together and then case w/ coir.

Joshua

Edited by Annom (12/21/04 10:19 AM)

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OfflineHammerloaf
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Registered: 11/26/01
Posts: 439
Loc: Canada
Last seen: 20 years, 7 months
Re: Puerto Rican Start [Re: Joshua]
    #536672 - 01/31/02 07:17 AM (22 years, 2 months ago)

Joshua.. try using a blender on those cakes, then "pour" your substrate layer into the pan...
#1 you'll be totally amazed at the recovery, and strength of the mycelium
#2 when you use the blender, and pour, you don't have to ever touch the substrate.

use a flat bladed blender with blades of differing heights....

Trust me on this one.

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Invisiblephrozendata
Carpal Tunnel

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Posts: 5,015
Re: Puerto Rican Start [Re: Hammerloaf]
    #536722 - 01/31/02 09:00 AM (22 years, 2 months ago)

Also, you could try breaking up the cake in a ziplock bag and let it sit in there for 24-48 hours to recover.

I have some PR jars colonizing but they ran into some trouble.

Just another brick wall in my face I guess.


--------------------
"There's only one corner of the universe you can be certain of improving and that's your own self. So you have to begin there, not
outside, not on other people" - Aldous Huxley

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InvisibleMajortrippz
enthusiast
Registered: 06/19/01
Posts: 199
Re: Puerto Rican Start [Re: phrozendata]
    #536753 - 01/31/02 09:44 AM (22 years, 2 months ago)

I just scooped them out of the jars with a spoon dipped in h2o2.

as far as pouring a blended substrate...are you saying add some water to it???or water +h2o2???

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OfflineLeGrouper
enthusiast
Registered: 01/22/02
Posts: 256
Last seen: 20 years, 4 months
Re: Puerto Rican Start [Re: Joshua]
    #536812 - 01/31/02 10:58 AM (22 years, 2 months ago)

You are so great a documenting your work! I really enjoy your posts! Thanks!


--------------------
The above post is entirely fictional and should not be taken out of context.

Ali-G
www.boyakasha.co.uk

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OfflineHammerloaf
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Registered: 11/26/01
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Loc: Canada
Last seen: 20 years, 7 months
Re: Puerto Rican Start [Re: Majortrippz]
    #536822 - 01/31/02 11:06 AM (22 years, 2 months ago)

Major.. no, the blended substrate doesn't require additives... perhaps a misting of H2O2 lightly before and after poured.. but thats about it.. the substrate you pour in will form a soild and I mean SOILD ASS block of mycelium within a couple of days.  Go mycelium go! :smile:

oh.. but do not blend whole grains.. only flour based cakes.

Edited by Hammerloaf (01/31/02 11:07 AM)

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InvisibleRyche HawkV
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Re: Puerto Rican Start [Re: Hammerloaf]
    #537919 - 02/01/02 03:20 PM (22 years, 1 month ago)

A blender ?? Are you kidding ??
How about just slicing the cakes neatly, then taking 1 cake to crumble it up and fill in the empty spaces.

You really should try and make your substrate layer more even next time


--------------------
-Peace-

High Quality MUSHROOM SPORES and CULTURES  for microscopy at www.muShrooms.com



muShrooms.com is the new web site of
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OfflineSyd_barret
The Greatest
Registered: 10/27/01
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Re: Puerto Rican Start [Re: Ryche Hawk]
    #538009 - 02/01/02 05:25 PM (22 years, 1 month ago)

Is there something wrong with using a blender?

I thought its best to crumble the cake as much as possible?

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OfflinePhred
Fred's son
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Re: Puerto Rican Start [Re: Joshua]
    #538103 - 02/01/02 07:36 PM (22 years, 1 month ago)

The Puerto Rican has by far the most aggressive mycelium I have ever seen. Nothing else even comes close. It?s like a fungal Pit Bull. I can?t see contams standing a chance with this stuff... it just colonizes too fast. I have seen it completely colonize the DRY verm barrier at the top of a jar, AND stick out of the inoculation holes, just as your pic shows.

Not only that, but it is insanely rhizomorphic. Big broad bands of mycelium everywhere. No tomentose growth at all. My jars looked like I had filled them with chopped up dental tape.

You should use a much deeper casing layer than you normally would because it will run through an inch-thick casing layer in no time... overlay is a real problem with Puerto Rican. As well, it can form some really gnarly looking fruits... they look sort of like amorphous blobs, or even mini-sclerotia. This is probably because it is still a pretty wild strain and not yet well-domesticated.

Potent as hell, though.

pinky



--------------------

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InvisibleJoshua
Holoman
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Registered: 10/27/98
Posts: 5,398
Loc: The Matrix
Re: Puerto Rican Start [Re: Phred]
    #538134 - 02/01/02 09:00 PM (22 years, 1 month ago)

What?s your advice on pinning/fruiting? I?ve heard this strain can be hard to fruit.

Joshua


--------------------
The Shroomery Bookstore

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"Life After Death is Saprophytic!"

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InvisibleZen Peddler
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Registered: 06/18/01
Posts: 6,379
Loc: orbit
Re: Puerto Rican Start [Re: Hammerloaf]
    #538139 - 02/01/02 09:04 PM (22 years, 1 month ago)

Blend it or break it up in a zip-lock baggie - either way works fine - as long as its even and broken up it all good...


--------------------

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InvisibleJoshua
Holoman
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Registered: 10/27/98
Posts: 5,398
Loc: The Matrix
Re: Puerto Rican Start [Re: Joshua]
    #538264 - 02/02/02 12:41 AM (22 years, 1 month ago)

This is two days later. I love the look of this, I hope y?all do too.






Joshua


--------------------
The Shroomery Bookstore

Great books for inquiring minds!

"Life After Death is Saprophytic!"

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OfflineSnobrdr311
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Registered: 09/03/01
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Re: Puerto Rican Start [Re: Zen Peddler]
    #538266 - 02/02/02 12:42 AM (22 years, 1 month ago)

my advise on PR casings is to not even let them incubate for a day, expose to light and start the fruiting phase right away.. or else they?ll overlay and start rotting for sure. this strain is super hard to pin, so start right away. it usually takes 10 days- 2 weeks to pin. sometimes you hit it and sometimes it?s harder, this strain is a total crapshoot, you never know what it wants to do.

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OfflineAzure
old hand
Registered: 12/31/98
Posts: 469
Loc: California, USA
Last seen: 21 years, 10 months
Re: Puerto Rican Start [Re: Joshua]
    #538311 - 02/02/02 02:01 AM (22 years, 1 month ago)

As you probably noticed, PR?s are very fast colonizers. Compared to KS, they take a little longer to pin. However, isolattes form massive pins-the most I?ve ever seen. Pinning won?t occur until the mycelium has drastically slowed down in vegetative growth. Cold shock is recommended when you start seeing rhizomorphs sticking out of the casing layer.

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InvisibleRoadkillM
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Registered: 12/11/01
Posts: 22,674
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Re: Puerto Rican Start [Re: Joshua]
    #538396 - 02/02/02 05:18 AM (22 years, 1 month ago)

Very nice Josh !

What kind of casing are you going to use?


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.


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OfflineAnnoA
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Re: Puerto Rican Start [Re: Joshua]
    #538415 - 02/02/02 07:37 AM (22 years, 1 month ago)

You should case this as soon as possible.

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OfflineHammerloaf
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Re: Puerto Rican Start [Re: Anno]
    #538590 - 02/02/02 12:35 PM (22 years, 1 month ago)

Mr. Hawke... if you?ve never used a blender on cakes... do it.. you?ll be amazed.. I swear. But remember, no whole grains, only flour based substrate, or something that was blended to begin with, like millet ground in a coffee grinder to make the cakes.

It works so damn good I?ll never go back.

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InvisibleMajortrippz
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Registered: 06/19/01
Posts: 199
Re: Puerto Rican Start [Re: Hammerloaf]
    #538647 - 02/02/02 02:27 PM (22 years, 1 month ago)

Honestly I don?t see why you all say these are so hard to fruit???

I?m on my fouth flush with 4 trays and 2nd with 2 and have gotten a total of 4 ounces(dry) so far form 6 pint cakes... when they slow down I just scatch the casing and drown the it with a 1-20 dilution of h2o2... let it sit for 1 day. then a new flush begins... pour of the excess water and presto the next great flush...I haven?t had a single contamination case.... I?m looking forward to say 6 flushes or so...oh yeah I?m talking 2 gram dry weight per fruit.

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InvisibleJoshua
Holoman
Male

Registered: 10/27/98
Posts: 5,398
Loc: The Matrix
Re: Puerto Rican Start [Re: Roadkill]
    #539099 - 02/03/02 04:57 AM (22 years, 1 month ago)

The casing will be applied very soon. I will apply a mix of:

%40 verm
%60 coir
and lime to taste.







Joshua


--------------------
The Shroomery Bookstore

Great books for inquiring minds!

"Life After Death is Saprophytic!"

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InvisibleJoshua
Holoman
Male

Registered: 10/27/98
Posts: 5,398
Loc: The Matrix
Re: Puerto Rican Start [Re: Anno]
    #539105 - 02/03/02 05:42 AM (22 years, 1 month ago)

Case solved---------|/

The casing mix is an officially Annonized mix.



Joshua


--------------------
The Shroomery Bookstore

Great books for inquiring minds!

"Life After Death is Saprophytic!"

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