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Offlineweezle
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Registered: 03/13/04
Posts: 13
Last seen: 17 years, 10 months
disapearing mycellium
    #5380087 - 03/09/06 02:09 AM (17 years, 10 months ago)

ok i have a pastuerized poop and hay right.... its 2" deep in a big tupperware container with tinfoil on top with holes in it. now after 3 days this thing looked like a spider had a feild day. now it seems to be disapearing. i realy dont know what to do. i put a ton of spores into it. enough to do the last 2 months of innoculating everyone here has done trust me on that. any suggestions?


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Offlineweezle
Stranger
Registered: 03/13/04
Posts: 13
Last seen: 17 years, 10 months
Re: disapearing mycellium [Re: weezle]
    #5380097 - 03/09/06 02:21 AM (17 years, 10 months ago)

i should stop coming here no one looks at my stuff and god gives me more replies


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OfflineDelinquentes
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Registered: 02/07/06
Posts: 543
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Last seen: 3 months, 16 days
Re: disapearing mycellium [Re: weezle]
    #5380107 - 03/09/06 02:25 AM (17 years, 10 months ago)

You waited EXACTLY 10 miutes at 4+AM EST to post a complaint that no one replied to a post that is barely passable English, and hard to understand.  With post's like this, it's no wonder you don't get an answer.

I'll keep it bumped for you so someone can answer it tomorrow.

:rolleyes:

God doesn't live here, but the "gods and goddeses" do.  Be patient.


--------------------
"The price of meat has just gone up,
and your old lady has just gone down!" ~~ Zappa



Click Here for a ghetto ozone generator Tek.

Want to learn more about ozone as a bacteria killer?  Click Here


Edited by Delinquentes (03/09/06 02:35 AM)


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InvisibleDarkenshroom
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Registered: 02/24/06
Posts: 683
Loc: I don't exist on this pla...
Re: disapearing mycellium [Re: weezle]
    #5380113 - 03/09/06 02:28 AM (17 years, 10 months ago)

First off it is hard to understand your situation without more information. Pics, Grow Log, Information on Methodologies used.

It is obvious that you are trying Bulk Substrate and that you used Spores to inoculate. 3 days from Spores should not have shown anything, unless of course you had a run of cobweb and then it died or receded inside. For bulk substrates I would suggest using a G2G transfer with some Rye, Corn, WBS, or other inoculated substrate.

Also I would suggest you look into the cultivation Sexton of the site, find some of the more useful teks that apply to what you are trying to do, and then stick to you tek, always always always until you are a experienced grower stick to your tek!

The amount of spores put onto the substrate may or may not have been a good things, remember spores come in moisture and excess moisture leads to mildew, rot, and contamination. Find a good tek, read, read, read, and if your current project fails (I can't predict that as I don't know your sterility conditions and whatnot) try again following more closely the outlined methods you choose to follow.


Darken
*smiles*


--------------------
~The first and most important step in cultivation of the wonderful mushroom, is the cultivation of patience for without it you doom yourself to failure~


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Invisibletiny_rabid_birds
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Registered: 11/08/05
Posts: 15,653
Loc: estados unidos
Re: disapearing mycellium [Re: Delinquentes]
    #5380122 - 03/09/06 02:33 AM (17 years, 10 months ago)

Delinquentes is right, cool your jets. Plus, what do you expect? It's the wee hours of the morning in the states, only crazies are up at this time.

Now my take on the matter is that you did not read, nor did you follow any tek. You cannot expect spores to germinate and mycelium to properly colonize pasteurized poo that has only been inoculated with spores, especially not within 3 days. When working with pasteurized bulk substrates, you need to use spawn - generally grain.

If you did follow a tek, please be so kind as to give us a link. If you didn't, please stop your bitching because it's very likely that barely anyone is awake to see your query, and those who did dismissed it as a stupid error not deserving to be dignified with a response.

Take no offense, just be nice and people will be nice in return. Be an impatient, ungrateful dick, and you get dicked in return. For future reference follow the teks, they're there for a reason.


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Invisibletiny_rabid_birds
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Re: disapearing mycellium [Re: Darkenshroom]
    #5380129 - 03/09/06 02:34 AM (17 years, 10 months ago)

Quote:

Darkenshroom said:
unless of course you had a run of cobweb and then it died or receded inside.




That's exactly what I was thinking.


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InvisibleDarkenshroom
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Re: disapearing mycellium [Re: tiny_rabid_birds]
    #5380135 - 03/09/06 02:36 AM (17 years, 10 months ago)

All that liquid and then the comment "like a spider had a field day with it" made me go COBWEB MOLD, and probably now all through your substrate.

Darken
*hates cobweb*


--------------------
~The first and most important step in cultivation of the wonderful mushroom, is the cultivation of patience for without it you doom yourself to failure~


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Offlineweezle
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Registered: 03/13/04
Posts: 13
Last seen: 17 years, 10 months
Re: disapearing mycellium [Re: tiny_rabid_birds]
    #5380162 - 03/09/06 02:48 AM (17 years, 10 months ago)

yeah ive been reading into this for years i did my own tek with some stuff stolen from others and only god knows where this one is going. i did take into consideration the amount of spore liquid i used to the amount of moisture in the poo. sorry im very impatient i usualy hit up the chat but it seems to be down. and i cant use grain because i do not have a pressure cooker. im putting things together with bits and peices that i have. actualy i was amazed when 3 days i had mycellial growth. but i did put in about 24 cc's of black spore syringes. to the light they were as dark as this blue background. i pastuerized the poop and hay cleaned the bin realy isnt anything more than that. i dont have a camera so i cant take pictures. wish i could though.


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Offlineweezle
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Registered: 03/13/04
Posts: 13
Last seen: 17 years, 10 months
Re: disapearing mycellium [Re: weezle]
    #5380169 - 03/09/06 02:50 AM (17 years, 10 months ago)

if its cobweb mold can i reuse the same stuff just repasturize it. or am i going to have to get all new materials?


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InvisibleDarkenshroom
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Registered: 02/24/06
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Re: disapearing mycellium [Re: weezle]
    #5380183 - 03/09/06 02:56 AM (17 years, 10 months ago)

I imagine that you over moistened and caused a run of cobweb mold, It has probably moved on to the interior of your project. Look up some of the microwave teks, or some of the steam teks, hell even oven teks.

If you are stuck working with straw and poo may I suggest Roger Rabbits Straw Log Tek. It can be foudn with the search engine, go to Advanced Mycology and look for the Entry "Dunking Straw Logs"

Since you seem to have access to straw and anyone can get a big tub, you might try that, the plastic sleeve material can be found at any home supply store that carries vacuum sealers. It comes in rolls and is cheap. SO is the straw. you would still need to inoculate from something so I would suggest since you have Poo, doing Poo Cakes, once colonized gently break up the poo into the straw to inoculate in place of grains.

Hope that helps some, and wish you luck.

Also on a side note, darker spore syringes does not make them better. It is best to use about 1.5th of a quarter sized print as per the teks on making syringes. you want the spores to have enough access to liquid to hydrate and you really don't need to see them all tat well if at all. remember one spore print has 100,000,000 or more spores. And that means even 1/5 a print would be more spores than would ever be needed.


Good luck and happy growing.

Darken
*smiles*


--------------------
~The first and most important step in cultivation of the wonderful mushroom, is the cultivation of patience for without it you doom yourself to failure~


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Invisibletiny_rabid_birds
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Re: disapearing mycellium [Re: weezle]
    #5380185 - 03/09/06 02:56 AM (17 years, 10 months ago)

Poo is free, I'd just trash the tray. And using more spore solution is not always a good idea. In fact, in multispore inoculation (which it is whenever you use a spore syringe/print) when you use an excessive amount of spores, it can actually hinder growth, as there will be so many more substrains battling it out.

You really need to spawn using another medium. It's extremely unlikely that you'll be successful merely inoculating pasteurized bulk substrate with spores. Since you don't have a PC, just do some PF jars and boil them, then use them as spawn.

I really don't know what else to tell you. If you continue using that manner of growing, I believe you'll be very unsuccessful, but that's just my take on the situation.


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InvisibleDarkenshroom
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Re: disapearing mycellium [Re: Darkenshroom]
    #5380189 - 03/09/06 02:57 AM (17 years, 10 months ago)

You would need to start over.

Darken
*smiles*


--------------------
~The first and most important step in cultivation of the wonderful mushroom, is the cultivation of patience for without it you doom yourself to failure~


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