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MustNotBe
HPPDer


Registered: 01/27/06
Posts: 930
Last seen: 1 year, 8 months
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Help On New Idea
#5377223 - 03/08/06 11:05 AM (17 years, 10 months ago) |
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I made a LC using a new method of mine, and its working well (2 days in). BUT I don't really feel that using it to inoculate jars would be helpful. So I was wonder if I could sterilize some substrate. I was going to use brf/verm for now, and order some 50/50+ mix from the new place (mediummania). But do you think that if I put a 1 1/2 inch layer of substrate on a baking pan (heated to 400 then cooled), and then pour the liquid culture over the whole layer, and put a 1 inch layer of damp verm that it would grow easily, and fast?
-------------------- Junkies United we stand , Devided we're sick as fuck. - - - "Hallucinations are something heroin users are not at all accustomed to," said Const. Conor King, Victoria police drug expert. "They react like you or I would react if we took Aspirin and all of a sudden the TV got up and started walking across the room." - - - Make drugs legal, or alcohol and tobacco illegal. Either way it's more fair.
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coda
Banjo Goiter


Registered: 03/20/01
Posts: 8,750
Last seen: 10 months, 3 days
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Re: Help On New Idea [Re: MustNotBe]
#5377244 - 03/08/06 11:12 AM (17 years, 10 months ago) |
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nope.
-------------------- To get really high is to forget yourself. And to forget yourself is to see everything else. And to see everything else is to become an understanding molecule in evolution, a conscious tool of the universe. And I think every human being should be a conscious tool of the universe. . . . -JG i really am glad you came back to us instead of taking the other path. *hug* -A_S (RIP your final words to me will never be forgotten)
 Don't fuck with the laughing jesus.
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thenewguy05
The Mushroom Man


Registered: 02/11/05
Posts: 2,123
Loc: My Underground Layer
Last seen: 2 years, 5 months
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Re: Help On New Idea [Re: coda]
#5377250 - 03/08/06 11:14 AM (17 years, 10 months ago) |
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AGREED!!! if there were shortcuts we would all be using them.
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musher_420
Stranger

Registered: 08/01/05
Posts: 2,691
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Re: Help On New Idea [Re: MustNotBe]
#5377251 - 03/08/06 11:15 AM (17 years, 10 months ago) |
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I see MANY potential problems with this. The answer your going to get from most people is no.
Try it out, see what happens.
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coda
Banjo Goiter


Registered: 03/20/01
Posts: 8,750
Last seen: 10 months, 3 days
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Re: Help On New Idea [Re: musher_420]
#5377254 - 03/08/06 11:16 AM (17 years, 10 months ago) |
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i wouldnt even bother trying it out, why waste all that good LC on something that will contam?
-------------------- To get really high is to forget yourself. And to forget yourself is to see everything else. And to see everything else is to become an understanding molecule in evolution, a conscious tool of the universe. And I think every human being should be a conscious tool of the universe. . . . -JG i really am glad you came back to us instead of taking the other path. *hug* -A_S (RIP your final words to me will never be forgotten)
 Don't fuck with the laughing jesus.
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musher_420
Stranger

Registered: 08/01/05
Posts: 2,691
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Re: Help On New Idea [Re: coda]
#5377267 - 03/08/06 11:21 AM (17 years, 10 months ago) |
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Learning experience?
Sure it's a waste of some time and a very tiny amount of resources but George Washington didn't invent the light bulb by sitting around and asking people if his experiments would work or not... (In fact I'm pretty sure he didn't invent the light bulb at all.)
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Premedman1
Assistant to the insistent


Registered: 12/21/05
Posts: 2,376
Loc: South of Sanity
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Re: Help On New Idea [Re: musher_420]
#5377290 - 03/08/06 11:33 AM (17 years, 10 months ago) |
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Quote:
musher_420 said: but George Washington didn't invent the light bulb by sitting around and asking people if his experiments would work or not... (In fact I'm pretty sure he didn't invent the light bulb at all.)
-------------------- Build a man a fire, he is warm for the night. Set a man on fire, he is warm for the rest of his life.
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MustNotBe
HPPDer


Registered: 01/27/06
Posts: 930
Last seen: 1 year, 8 months
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Re: Help On New Idea [Re: coda]
#5377332 - 03/08/06 12:04 PM (17 years, 10 months ago) |
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Its so SO easy to make LC's, that it's not really a waste.
-------------------- Junkies United we stand , Devided we're sick as fuck. - - - "Hallucinations are something heroin users are not at all accustomed to," said Const. Conor King, Victoria police drug expert. "They react like you or I would react if we took Aspirin and all of a sudden the TV got up and started walking across the room." - - - Make drugs legal, or alcohol and tobacco illegal. Either way it's more fair.
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coda
Banjo Goiter


Registered: 03/20/01
Posts: 8,750
Last seen: 10 months, 3 days
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Re: Help On New Idea [Re: MustNotBe]
#5377338 - 03/08/06 12:09 PM (17 years, 10 months ago) |
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it's a waste when a perfectly good LC is put into use via a project that's doomed to fail.
-------------------- To get really high is to forget yourself. And to forget yourself is to see everything else. And to see everything else is to become an understanding molecule in evolution, a conscious tool of the universe. And I think every human being should be a conscious tool of the universe. . . . -JG i really am glad you came back to us instead of taking the other path. *hug* -A_S (RIP your final words to me will never be forgotten)
 Don't fuck with the laughing jesus.
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dedjam
Electro Penguin



Registered: 12/14/05
Posts: 2,139
Loc: Moralton, Statesota
Last seen: 12 years, 8 months
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Re: Help On New Idea [Re: coda]
#5377343 - 03/08/06 12:13 PM (17 years, 10 months ago) |
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I am all about experimenting with new things, but you need to approach new ideas a bit more scientifically/logically. You need to atleast EXPECT something to have a chance, and there is no expectation of succeeding here.
Try it, make a thread about it, and then when someone else comes along with the same idea, they can see specifically how it will not work. That way it isnt a total loss atleast, as it may help out someone that wants to try it in the future.
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musher_420
Stranger

Registered: 08/01/05
Posts: 2,691
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Re: Help On New Idea [Re: dedjam]
#5377369 - 03/08/06 12:28 PM (17 years, 10 months ago) |
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Ok here is an idea,
What if you took two trays that are suitable for the oven. Filled the bottom tray up with a substrate of let's say, a mixture of grains, horse poo, and maybe some coir to help speed up colonization.
Once the substrate is at proper moistrue content, place the second tray on top of the first one and sealed the two together with micropore tape.
Then you weighed the whole the thing.
Maybe poke a few holes in the top tray and cover those with micropore tape. Then place into the oven at 250 degrees ferinheight for lets say two hours.
After that time is up you remove it from the oven allow it to cool and weigh it once again. Find the differnce in weight and this should be how much water was lost during "sterilization". ( 1g = 1mL of water)
Once you calcuate the volume of water that was lost during "sterlization" add that same volume of LC using a LARGE syringe (availabe at farm supply stores)
Biggest problem I see with this is the substrate burning.
Edited by musher_420 (03/08/06 12:35 PM)
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coda
Banjo Goiter


Registered: 03/20/01
Posts: 8,750
Last seen: 10 months, 3 days
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Re: Help On New Idea [Re: musher_420]
#5377394 - 03/08/06 12:40 PM (17 years, 10 months ago) |
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you cant sterilize grains in the oven, introducing that much LC to an unsterilized substrate is just asking for contams. Having uncolonized grains exposed to the elements is just asking for contams, using liquid culture on unsterilized substrate will just lead to contams.
See where im going with this? You're not creating a sterile environment, your substrate will be exposed to open air, and you will have uncolonized grains littered throughout the substrate. All of those factors, on their own or combined, will lead you down the path to failure.
If anything you need to make up some spawn bags and go that route, even then to be truly successful YOU WILL NEED A PC.
-------------------- To get really high is to forget yourself. And to forget yourself is to see everything else. And to see everything else is to become an understanding molecule in evolution, a conscious tool of the universe. And I think every human being should be a conscious tool of the universe. . . . -JG i really am glad you came back to us instead of taking the other path. *hug* -A_S (RIP your final words to me will never be forgotten)
 Don't fuck with the laughing jesus.
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