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Offlineblackout
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Registered: 07/16/00
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LC in a thick potato/rice flour/grain flour broth
    #5373324 - 03/07/06 04:22 AM (17 years, 10 months ago)

Has anybody grown LCs in very thick broths. The limiting factor in sugar broths is the % of sugars, too high % will kill the spores, there is no such limiting factor with starches. If you made a very thick potato broth with instant flakes you may get and extremely dense LC. So dense that it could possibly be used to inoculated semi-dry pasteurised bulk substrates. Since it would have no sugars it should not be a prime site for contams. I would simply pour the thick LC on manure and mix it up well.

Any reasons this wouldn't work?


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Offlinemogur
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Re: LC in a thick potato/rice flour/grain flour broth [Re: blackout]
    #5373343 - 03/07/06 04:52 AM (17 years, 10 months ago)

I'm no expert (agar is, of course), but my trials show that even though it started slow, 25% karo populated the thickest of five strengths that I tried. With the starches that I tried, most were so thick that they were too cloudy to see through, and you couldn't tell myc growth from contams. Even if it wasn't contams, the thick starches did not produce thick growth.

My latest LC is 5% karo, 15% clarified potato infusion. Today is the second day, and they all have very heavy growth, even the spore noc'd jars. They could be used already if I was in a hurry.


Edited by mogur (03/07/06 04:55 AM)


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Re: LC in a thick potato/rice flour/grain flour broth [Re: mogur]
    #5373392 - 03/07/06 06:31 AM (17 years, 10 months ago)

I would expect 25% karo to show no growth at all. Like yeast, if you put spores in too strong a solution they simply will not grow. Are you sure it was 25% karo? that must be like syrup.

Somebody pm'd me recently saying Workman was using 25% but it turned out to be

Stock solution (100%)
250ml potato water from boiled potatoes
1 tsp dextrose powder from the brew store

so 25% is really that above solution diluted down, so there is very little dextrose in there.

What exactly do you mean by 15% clarified potato infusion?
I made a broth myself of potato water with flakes and it is hard to tell if growth is happening.


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