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OfflineBIGUN
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Registered: 02/23/06
Posts: 10
Last seen: 12 years, 10 months
Did I "F" up? nub LC question
    #5370398 - 03/06/06 10:52 AM (18 years, 27 days ago)

This is my first LC, and my first grow. I have been reading over these boards for a couple weeks now and decided to give it a shot. I used the :LIQUID CULTURE - THE EASY WAY: method, but when i sterilized the syringe with an alcohol lamp I didn't wait for it to cool down before injecting the baby bottles. They have been incubating at around 70-75 degrees for a little over a week and they show no signs of mycelium growth. I built an incubator which stays at a constant 80 degrees and put the bottles in there to see if it was just the low temp but that was last night so no changes yet.

This leads my to my next question, if the spores did die from the hot syringe can I take those bottles and inject them with another strain?
The first syringe was Z-Strain, but I think they all died, all that I can see in the bottle is the tiny black specs at the bottom.
But would I be able to inject those same bottles with a Koh Sumui syringe?

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OfflineFraggin
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Registered: 01/05/05
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Re: Did I "F" up? nub LC question [Re: BIGUN]
    #5370423 - 03/06/06 10:56 AM (18 years, 27 days ago)

Do it all over if you have doubts.

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OfflineSinthetic
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Registered: 12/05/05
Posts: 812
Last seen: 15 years, 8 months
Re: Did I "F" up? nub LC question [Re: Fraggin]
    #5370575 - 03/06/06 11:45 AM (18 years, 27 days ago)

Just do it over. This hobby doesn't tolerate many shortcuts.

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OfflineBIGUN
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Registered: 02/23/06
Posts: 10
Last seen: 12 years, 10 months
Re: Did I "F" up? nub LC question [Re: Sinthetic]
    #5372454 - 03/06/06 09:30 PM (18 years, 26 days ago)

one more question, my PC is on the smaller side and the baby bottles don't fit in standing up. Does it matter if they are tilted in the PC?

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InvisibleAtheist
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Registered: 01/24/06
Posts: 13,705
Loc: USA
Re: Did I "F" up? nub LC question [Re: BIGUN]
    #5372464 - 03/06/06 09:34 PM (18 years, 26 days ago)

Nope. Just as long as they dont leak or get fucked up from boiling water they will be fine.

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