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Invisibleagar
old hand
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Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
Trusted Cultivator
CLEAN WORK
    #5368509 - 03/05/06 08:07 PM (17 years, 10 months ago)



Clean clothes & work-place, along with good sanitary procedures.

:tongue2:AND :tongue2:

Hair covering (net/cap), face mask, exam gloves & Lysol/Oust.

ARE YOUR BEST FRIENDS.

These are common-place inexpensive items, found at most local drug/pharmacy stores.

Using these will cut down on contamination.

I am amazed, why many don't use them. :confused: :stoned:
Then wonder why their jars/bags, syringes, prints or whatever get contaminated. :eek: :mad2: :sad:


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OfflineSnaggletooth
Stranger in a Strange Land
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Registered: 10/24/05
Posts: 6,109
Loc: blinks stupidly
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Re: CLEAN WORK [Re: agar]
    #5368548 - 03/05/06 08:19 PM (17 years, 10 months ago)

Yeap.........No Glove no Love :tongue:

Play safe people  :wink:


--------------------


Atheist Chat


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InvisibleDarkenshroom
PsychedelicExplorationist
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Registered: 02/24/06
Posts: 683
Loc: I don't exist on this pla...
Re: CLEAN WORK [Re: agar]
    #5368549 - 03/05/06 08:22 PM (17 years, 10 months ago)

3 Cheers Agar.

Simple steps can go a long way, I haven't even begun my first grow yet as I am still working on a clean set up and procedure plan worthy of a good grow log. I will be picking up materials to construct a glove box, as well as clean clothes, hair nets, gloves, and sprays this week, as well as finishing up my martha and replacing any integral parts on the pc I bought.

Sterile work environments in a hobby as contaminant vulnerable as ours is paramount.

Darken
*smiles*


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~The first and most important step in cultivation of the wonderful mushroom, is the cultivation of patience for without it you doom yourself to failure~


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Invisiblemusher_420
Stranger

Registered: 08/01/05
Posts: 2,691
Re: CLEAN WORK [Re: agar]
    #5368570 - 03/05/06 08:35 PM (17 years, 10 months ago)

I think a lot of beginners (I know I did!) have trouble recognizing the difference between sterility and cleanliness. And when each one is needed.

People need to realize
When working with PC'ed (sterilized) substrates sterility is EVERYTHING. Absolutely nothing but the mushroom's spores can be added to the jars.

WHICH MEANS, When you take your spores on a journey from cap to print to syringe to jar you better make sure no meanies hitch a ride with ya.

A glove box and isopropanol alcohol go along way IMO.

However, Sometimes you can go overboard. Like working with pasteurized substrates and fully colonized substrates. This is when only cleanliness is required. I think a lot of people out there are unnecessarily choking themselfs and spending money on cans of Lysol and the like.


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My Humidifier Reservoir Expansion Tek

Must read links for beginners

Btw- You are not what you own!


Edited by musher_420 (03/05/06 08:36 PM)


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OfflineDelinquentes
TIMMMAYYYYYYY
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Registered: 02/07/06
Posts: 543
Loc: Hidin' in a bowl of rice
Last seen: 3 months, 16 days
Re: CLEAN WORK [Re: musher_420]
    #5368581 - 03/05/06 08:39 PM (17 years, 10 months ago)

Great infomation as usual.


Thanks for taking the time.


--------------------
"The price of meat has just gone up,
and your old lady has just gone down!" ~~ Zappa



Click Here for a ghetto ozone generator Tek.

Want to learn more about ozone as a bacteria killer?  Click Here


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InvisiblePsychoslut
The Mother Fucking Bear-o-dactyl
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Registered: 12/10/02
Posts: 20,917
Loc: all up in ya
Re: CLEAN WORK [Re: Snaggletooth]
    #5368660 - 03/05/06 09:16 PM (17 years, 10 months ago)

Quote:

Snaggletooth said:
Yeap.........No Glove no Love :tongue:

Play safe people  :wink:





I hate latex gloves i just use allot of hand sanitizing gell. `


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


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OfflineRogerRabbitM
Bans for Pleasure
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Registered: 03/26/03
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Loc: Seattle
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OG Cultivator
Re: CLEAN WORK [Re: Psychoslut]
    #5368861 - 03/05/06 10:13 PM (17 years, 10 months ago)

Growers must realize that WE are the biggest source of contamination.

I'll add to the above not to talk or sing, etc., while inoculating jars or doing other clean work. Your breath leaving your mouth is traveling faster even than a flow hood can blow it back. It's also perhaps the largest source of bacteria in our jars. The bests surgical masks will stop 99% of the bacteria leaving your mouth, but think about that 1% of several billion that gets through. That's a lot of bacteria even with a good medical, not dust mask. I hold my breath anytime a jar or petri dish is open. Gloves are mandatory for consistent success. A box of them is less than the price of a single spore syringe. They'll save a lot of failures.
RR


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Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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Offlinetokey666
Let's Build It Instead
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Registered: 08/05/05
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Re: CLEAN WORK [Re: Psychoslut]
    #5368869 - 03/05/06 10:14 PM (17 years, 10 months ago)

AGREED!

And the funny thing is....its not that hard to be sterile! A spray spray there, a wipe wipe here, and you're off to the land of oz. :smile:


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Offlinemikeownow
Humungus fungus

Registered: 09/01/05
Posts: 2,856
Loc: WA,USA
Last seen: 17 years, 3 months
Re: CLEAN WORK [Re: tokey666]
    #5368899 - 03/05/06 10:22 PM (17 years, 10 months ago)

I have been quite lazy and infact have done my inoculations in some of the poorist of sterility and yet I have a high success rate so far. But better safe than sorry in my book.


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No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.


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InvisiblePsychoslut
The Mother Fucking Bear-o-dactyl
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Registered: 12/10/02
Posts: 20,917
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Re: CLEAN WORK [Re: RogerRabbit]
    #5368982 - 03/05/06 10:46 PM (17 years, 10 months ago)

I dont do to much with petris. For a standard inoculation in a quart jar you just need to be fast. I dont use a glove box or air cleaning crap and i get about 1 of 30 jars conataminate.


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


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Offlinemycodetour
Mycologist inTraining

Registered: 03/02/06
Posts: 136
Loc: WA
Last seen: 17 years, 8 months
Re: CLEAN WORK [Re: Psychoslut]
    #5369016 - 03/05/06 10:55 PM (17 years, 10 months ago)

Incubating casings in a clean environment and not opening to check them is one major thing I just recently learned the hard way. I introduced the green meanie to 4 of the 6 casings I have been incubating. Total devistation... I'm hoping the other two stay strong and dont get contammed but I dont have much hope. I know for sure that next time I case I will NOT open to check until atLEAST a full week. As well as store them in an sanitized, opaque tub (not incubator, and not just covered with foil and a blanket over.) Like I said, I learned the hard way but some times that's what it takes. Learn from other's mistakes if you haven't already learned from yours though.. thats the best way to go. I wish I didn't blow off basic steps such as spraying lysol before checking on casings, using gloves to spawn the poo, next time I'm even spawning in my glove box.. I refuse to have another contamination outbreak... Most of the work I have done up to this point is now outside in the dumpster. Back to the Jar waiting game...


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my set up / log


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InvisiblePsychoslut
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Re: CLEAN WORK [Re: mycodetour]
    #5369036 - 03/05/06 11:00 PM (17 years, 10 months ago)

Use clear lids or saran wrap and you can check on them without introducing mold spores.


But you are right i think fucking with it too much is the worst noob mistake.


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


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Offlinemycodetour
Mycologist inTraining

Registered: 03/02/06
Posts: 136
Loc: WA
Last seen: 17 years, 8 months
Re: CLEAN WORK [Re: Psychoslut]
    #5369051 - 03/05/06 11:03 PM (17 years, 10 months ago)

Yea I will use a big opaque clean, tub with a clear lid and cover the lid with something so not to expose it to any light


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my set up / log


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Invisibleohmatic
searcher
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Registered: 02/28/04
Posts: 6,742
Loc: europe
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Re: CLEAN WORK [Re: mycodetour]
    #5369582 - 03/06/06 02:01 AM (17 years, 10 months ago)

you know, i dont have a hairnet, but i usually just strap over a clean pair of boxershorts :wink:

i soooo remember that one time i was having boxers on my head any my roomie came home,
he was like "Well, your wearing shots on your head .." - "Yeah i know .. im casing" :wink:
peace ohm :mushroom2:


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:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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OfflineHippieChick
Chicks can do it too!
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Registered: 02/20/05
Posts: 5,958
Loc: Midwest
Last seen: 3 years, 13 days
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Re: CLEAN WORK *DELETED* [Re: Snaggletooth]
    #5369634 - 03/06/06 02:24 AM (17 years, 10 months ago)

Post deleted by HippieChick

Reason for deletion: .



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Peace,Love and Happiness
:heart: HC :mushroom2:

Freedoms just another word for nothing left to lose..............

I LUV My Greenhouse
http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848

My First Pans
http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058


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Invisibletiny_rabid_birds
Nocturnal
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Registered: 11/08/05
Posts: 15,653
Loc: estados unidos
Re: CLEAN WORK [Re: HippieChick]
    #5369660 - 03/06/06 02:45 AM (17 years, 10 months ago)

Gah.. I wish I were naturally a clean freak.  But I'm not.  I'm a total slob.  I haven't taken the trash or recyclables out of my dormroom in at least a month and a half.  My car is such a wreck that there's litterally trash/junk piled up passed the seats in the back.  I buy plastic silverware because I never wash regular ones, and I've got empty food packets, pudding containers, and fruit refuse just lying on various surfaces in my room.. it's bad.

So all the cleanliness required for this hobby is not easy for me.  And I've had my fair share of contams from cutting corners or just being generally unsterile.  But I think I'm progressing.. lol.  Hopefully this hobby will be my turning point and will lead me to start living in a cleaner environment.  Because frankly, my filthiness sickens me.  :nonono:


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OfflineDelinquentes
TIMMMAYYYYYYY
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Registered: 02/07/06
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Re: CLEAN WORK [Re: tiny_rabid_birds]
    #5369674 - 03/06/06 02:51 AM (17 years, 10 months ago)

TRB, that's hard core, man! There are times I can relate being a bachelor and all, but daaaayyyyuuuummm.


--------------------
"The price of meat has just gone up,
and your old lady has just gone down!" ~~ Zappa



Click Here for a ghetto ozone generator Tek.

Want to learn more about ozone as a bacteria killer?  Click Here


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InvisibleRoadkillM
Retired Shroomery Mod
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Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: CLEAN WORK [Re: agar]
    #5369717 - 03/06/06 03:06 AM (17 years, 10 months ago)

I'm with Agar, RR and HC on this!~



I sometimes forget my gloves.

:smirk:


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Invisibleagar
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Registered: 11/21/04
Posts: 9,056
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Re: CLEAN WORK [Re: HippieChick]
    #5369845 - 03/06/06 04:42 AM (17 years, 10 months ago)

Quote:

HippieChick said:
Quote:

Snaggletooth said:
Yeap.........No Glove no Love :tongue:

Play safe people  :wink:







OK . I always do.......





I'm very anal about it  :wink: LMAO

I like to keep my work area so clean you could EAT off it  :grin:

Peace,Love,Happiness and Harmony
:heart: Hippie Chick  :mushroom2:




LOL :rolleyes: :grin: :tongue:


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Offlinemycodetour
Mycologist inTraining

Registered: 03/02/06
Posts: 136
Loc: WA
Last seen: 17 years, 8 months
Re: CLEAN WORK [Re: agar]
    #5369856 - 03/06/06 04:54 AM (17 years, 10 months ago)

:rimshot:


--------------------
my set up / log


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