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Offlineblackout
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Registered: 07/16/00
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Last seen: 4 months, 12 days
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Selective agar recipies?
    #5246281 - 01/31/06 10:43 AM (18 years, 1 month ago)

I know there is anti bacterial agar available. Does anybody use an easy selective agar for cubes? like one with no sugars at all, maybe just manure or rice or potato flour?

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Invisiblemicololo2
Stranger

Registered: 11/05/05
Posts: 388
Loc: Québec, Canada
Re: Selective agar recipies? [Re: blackout]
    #5246614 - 01/31/06 12:06 PM (18 years, 1 month ago)

One of my home made agar receipe for woodlover:
Natural agar, brewer's yeast, home made wheat gely, 26% protein dry chicken cat food, 2 pellets of hardwood sawdust per 500 ml.
It's the best I've found trew the years.
The grow on wheat grain and sawdust look enhance with that receipe.

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Offlinegourmetgrower
I'ze the milkmanwhat knocked upyer maw

Registered: 01/29/06
Posts: 170
Loc: The Old West
Last seen: 18 years, 8 days
Re: Selective agar recipies? [Re: micololo2]
    #5248962 - 01/31/06 09:51 PM (18 years, 1 month ago)

It is easy to mix hydrogen peroxide in with your agar. Mix it in when the agar is cool enough to touch the jar, but not yet solidifying. Too much heat will destroy the peroxide. A little peroxide goes a long way.

This will produce an agar that will kill both bacteria and spores. It can be used for mycelial propogation only. It pretty much allows for inoculation with myc. in dirty environments (eg. taking a sample from a mushroom you find outside).

Edited by gourmetgrower (02/01/06 09:40 PM)

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Invisibleshymanta
Mad Scientist
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Registered: 01/27/05
Posts: 907
Re: Selective agar recipies? [Re: blackout]
    #5355718 - 03/01/06 11:14 PM (18 years, 21 days ago)

I made some agar with brown rice flour. Works pretty good. My next batch will be interesting. I recently made some substrate with quinoa and there was a lot of thick juice from the grain after simmering. I saved the juice and pc'd in a jar. I will use this as a nutritive base for the agar. I'm experimenting with quina because I read that it has tryptamine in it. Don't know if that is true but if it is the implications are interesting to say the least.

If I ever pasteurize poo via the pillowcase method, I will be saving the water for agar preparation.

Edited by shymanta (03/05/06 07:51 PM)

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InvisiblePremedman1
Assistant to the insistent
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Registered: 12/21/05
Posts: 2,376
Loc: South of Sanity
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Re: Selective agar recipies? [Re: gourmetgrower]
    #5358223 - 03/02/06 04:29 PM (18 years, 21 days ago)

Quote:

gourmetgrower said:
A little peroxide goes a long way.

This will produce an agar that will kill both bacteria and spores.




H2O2 :thumbup:


--------------------
Build a man a fire, he is warm for the night. Set a man on fire, he is warm for the rest of his life.

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Invisiblemicololo2
Stranger

Registered: 11/05/05
Posts: 388
Loc: Québec, Canada
Re: Selective agar recipies? [Re: shymanta]
    #5362216 - 03/03/06 04:44 PM (18 years, 20 days ago)

Quote:

shymanta said:
My next batch will be interesting. I recently made some substrate with quina and there was a lot of thick juice from the grain after simmering.




Looks like the samething of what I said 2 threads above "the home made wheat gely".

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Invisibleshymanta
Mad Scientist
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Registered: 01/27/05
Posts: 907
Re: Selective agar recipies? [Re: micololo2]
    #5368456 - 03/05/06 07:49 PM (18 years, 18 days ago)

Yeah, I'll be using a modified PDA recipe. Replacing the potato extract with quinoa extract.

Agar, "quinoa jelly", Karo, & yeast. '

That's my basic recipe, I just switch out the main nutrient to give my cultures a variety and keep them healthy.

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