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InvisibleFungusMan
I81U812
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Registered: 08/06/05
Posts: 3,112
Loc: Everywhere
Printing...
    #5368004 - 03/05/06 04:52 PM (17 years, 10 months ago)

When I made prints recently, I wasnt that sterile. I wore sterile gloves, and they printed in a container on aluminum, but no other safeguards were used. I was wondering if I could still make syringes safely by using a peroxide solution. Here is a print with a quarter circled in red next to it...


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InvisiblePsychoslut
The Mother Fucking Bear-o-dactyl
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Registered: 12/10/02
Posts: 20,917
Loc: all up in ya
Re: Printing... [Re: FungusMan]
    #5368014 - 03/05/06 05:01 PM (17 years, 10 months ago)

Peroxide destroys spores, just drop 15 bux on a vendor syringe.


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


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Invisibleagar
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Re: Printing... [Re: FungusMan]
    #5368015 - 03/05/06 05:02 PM (17 years, 10 months ago)

H202 & spores is not a good mix. It will KILL them.


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Invisiblecappa
Nerd
Registered: 02/12/06
Posts: 854
Re: Printing... [Re: agar]
    #5368046 - 03/05/06 05:17 PM (17 years, 10 months ago)

Not to nit pick here but isn't the foil supposed to be flat? LOLz!

Probably more work than is worth the while to dink around with a contam print.

Lessons learned right? I have yet to make a print myself. I'm looking forward to it, but at the same time... not so easy to make a sterile one before you have procedures in place. Gonna have to do some rehearsing to work the bugs out so I can do it right the first time.

~Cappa.


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Their are 10 types of people. Those that understand binary, and those who don't.
~Cappa.


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OfflineDelinquentes
TIMMMAYYYYYYY
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Registered: 02/07/06
Posts: 543
Loc: Hidin' in a bowl of rice
Last seen: 3 months, 16 days
Re: Printing... [Re: agar]
    #5368100 - 03/05/06 05:41 PM (17 years, 10 months ago)

Quote:

agar said:
H202 & spores is not a good mix. It will KILL them.




*Sheepishly dumps out his very first LC.*

THAT would explain why it's got clear water after 2 weeks.


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"The price of meat has just gone up,
and your old lady has just gone down!" ~~ Zappa



Click Here for a ghetto ozone generator Tek.

Want to learn more about ozone as a bacteria killer?  Click Here


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Invisiblemusher_420
Stranger

Registered: 08/01/05
Posts: 2,691
Re: Printing... [Re: Delinquentes]
    #5368153 - 03/05/06 06:09 PM (17 years, 10 months ago)

A still air glove box (without gloves) like this one
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/4359637/an/0/page/0 (Thanks Roadkill)

Is cheap and easy to make. It greatly improves sterility when working with spores.

Of course you can still make clean prints and syringes like this
http://www.shroomery.org/forums/showflat.php/Number/5276177#Post5276177 (Thanks Agar)

I use this method occasionally when I don't feel like cleaning my glove box and just want to make a good syringe. I put one print into a syringe and use that as a master syringe that can be diluted or used with LC's.


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My Humidifier Reservoir Expansion Tek

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Btw- You are not what you own!


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