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Delinquentes
TIMMMAYYYYYYY


Registered: 02/07/06
Posts: 543
Loc: Hidin' in a bowl of rice
Last seen: 3 months, 16 days
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How much LC do you use?
#5367556 - 03/05/06 02:19 PM (17 years, 10 months ago) |
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For those who use LC. How much of the white fluff needs to be in each injection site for the sample to be viable? Does there only need to be a speck? Do I need to make sure I have tons? I am gearing up the clean room to inoculate some popcorn I had prepared a few days ago. I only hope I am able to aspirate enough fluff in the smaller needles that I have.
Options and opinions welcome.
-------------------- "The price of meat has just gone up, and your old lady has just gone down!" ~~ Zappa Click Here for a ghetto ozone generator Tek. Want to learn more about ozone as a bacteria killer? Click Here
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mycodetour
Mycologist inTraining

Registered: 03/02/06
Posts: 136
Loc: WA
Last seen: 17 years, 8 months
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Re: How much LC do you use? [Re: Delinquentes]
#5367565 - 03/05/06 02:22 PM (17 years, 10 months ago) |
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i use as much as i can without messing up the water conent in the jars. i knocked up quart jars of rye berries with 10cc of lc and growth started in 24 hours.. the more you use, the quicker it colonizes.. but just a few flakes of myc should work if you are worried about running out
-------------------- my set up / log
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joker_66599
NOOB / SMALLTIME GROWER


Registered: 07/18/05
Posts: 758
Last seen: 3 years, 11 months
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Re: How much LC do you use? [Re: Delinquentes]
#5367570 - 03/05/06 02:24 PM (17 years, 10 months ago) |
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i think i was told more faster your jars will get going but i used 2-3cc a jar.
I just shook the hell out of the baby bottle and then sucked shit up.
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?¿ ¿? I don't really grow mushrooms I just act like it to be coo. ?¿ ¿?
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Delinquentes
TIMMMAYYYYYYY


Registered: 02/07/06
Posts: 543
Loc: Hidin' in a bowl of rice
Last seen: 3 months, 16 days
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Re: How much LC do you use? [Re: joker_66599]
#5367591 - 03/05/06 02:31 PM (17 years, 10 months ago) |
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I will be taking this LC from my pint jar I posted pictures of. As of yet, I only have 2BB's with anything resembling mycelium.
I might need to wait it out a little and hope for more mycelium to be generated in the pint jar though.
Thanks!
-------------------- "The price of meat has just gone up, and your old lady has just gone down!" ~~ Zappa Click Here for a ghetto ozone generator Tek. Want to learn more about ozone as a bacteria killer? Click Here
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joker_66599
NOOB / SMALLTIME GROWER


Registered: 07/18/05
Posts: 758
Last seen: 3 years, 11 months
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Re: How much LC do you use? [Re: Delinquentes]
#5367618 - 03/05/06 02:44 PM (17 years, 10 months ago) |
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i had 3 bb i used some out of 2 of them and th 3rd one is still sitting in my closet was the size of a hole in the middle of a cd not it's bigger than a golf ball took it about a month but grew huge
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?¿ ¿? I don't really grow mushrooms I just act like it to be coo. ?¿ ¿?
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: How much LC do you use? [Re: Delinquentes]
#5367697 - 03/05/06 03:09 PM (17 years, 10 months ago) |
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One of the problems with an LC.
Is that it can grow into a thick mass, that will plug standard gauge needles.
The second problem is breaking up those myc clumps.
The best way to do that is with a magnetic stir plate & stir bar.
  
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Delinquentes
TIMMMAYYYYYYY


Registered: 02/07/06
Posts: 543
Loc: Hidin' in a bowl of rice
Last seen: 3 months, 16 days
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Re: How much LC do you use? [Re: agar]
#5367708 - 03/05/06 03:13 PM (17 years, 10 months ago) |
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Agar,
The stuff I have is very fine....especially since I have shaken it vigorously once a day. I think this stuff will suck up in to the needle, but won't know till the syringes I just PC'ed cool down.
I guess what I really wanted to know is this: Will the tiniest piece of mycelium be enough to get colonization going or do I need a syringe FULL of mycelium to get the job done?
-------------------- "The price of meat has just gone up, and your old lady has just gone down!" ~~ Zappa Click Here for a ghetto ozone generator Tek. Want to learn more about ozone as a bacteria killer? Click Here
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: How much LC do you use? [Re: Delinquentes]
#5367756 - 03/05/06 03:26 PM (17 years, 10 months ago) |
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The more MYC particles in the syringe, THE BETTER.
Mine is near a slurry.
Try to get an LC as THICK, as possible.
If you only have a few small balls of myc fluff in a jar.
Try to suck those balls into a syringe.
Then, shake the HELL out of the syringe, to break them up even smaller.
The more myc particulates in a syringe, the more points of inoculation are created.
More contact points = THE BETTER.
Next time, try tubing filters on glass LC jars. With GAS EXCHANGE, you will get thicker LC's.
 The LC above, has a tiny hepa filter on the lid. It was NEVER agitated, other than a mild swirl, once in awhile.
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Delinquentes
TIMMMAYYYYYYY


Registered: 02/07/06
Posts: 543
Loc: Hidin' in a bowl of rice
Last seen: 3 months, 16 days
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Re: How much LC do you use? [Re: agar]
#5367843 - 03/05/06 03:53 PM (17 years, 10 months ago) |
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I see. Well, I have decided to wait it out a few days, and contiune to agitate the jar. I am not in any real hurry, I was just bored 
I guess I will build my fruiting chamber in preperation for my firt order of tennstudd goodness.
Thanks again, folks!
-------------------- "The price of meat has just gone up, and your old lady has just gone down!" ~~ Zappa Click Here for a ghetto ozone generator Tek. Want to learn more about ozone as a bacteria killer? Click Here
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Snaggletooth
Stranger in a Strange Land


Registered: 10/24/05
Posts: 6,109
Loc: blinks stupidly
Last seen: 6 years, 8 months
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Re: How much LC do you use? [Re: Delinquentes]
#5367895 - 03/05/06 04:06 PM (17 years, 10 months ago) |
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Here is a handy toy for LC 

Socorex - 0.5 to 5.0 ml w/feed tube
Agar turn me on to it
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Atheist Chat
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Delinquentes
TIMMMAYYYYYYY


Registered: 02/07/06
Posts: 543
Loc: Hidin' in a bowl of rice
Last seen: 3 months, 16 days
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Re: How much LC do you use? [Re: Snaggletooth]
#5367942 - 03/05/06 04:23 PM (17 years, 10 months ago) |
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Yeah, I like that beast. That will be when I take this to the next level. Once I have the opportunity to sample some of the fruits of may labor (pun intended) I am sure I am going to step up to the tools the professionals use. First thing will be a stir-plate and related equipment. I can use that as a multi-tasker for my other chemistry related endevors.
Snaggle - Can you PM me the details on that devil so I may locate and track one?
-------------------- "The price of meat has just gone up, and your old lady has just gone down!" ~~ Zappa Click Here for a ghetto ozone generator Tek. Want to learn more about ozone as a bacteria killer? Click Here
Edited by Delinquentes (03/05/06 04:26 PM)
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Snaggletooth
Stranger in a Strange Land


Registered: 10/24/05
Posts: 6,109
Loc: blinks stupidly
Last seen: 6 years, 8 months
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Re: How much LC do you use? [Re: Delinquentes]
#5367964 - 03/05/06 04:35 PM (17 years, 10 months ago) |
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Same here.......Soon after my first harvest I new I was going to be around for a while. 
you can check this out Socorex - 0.5 to 5.0 ml w/feed tube
I still need to get a stirrer and stuff but toys are fun
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Atheist Chat
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: How much LC do you use? [Re: Snaggletooth]
#5368011 - 03/05/06 04:56 PM (17 years, 10 months ago) |
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Quote:
Snaggletooth said: Here is a handy toy for LC 

Socorex - 0.5 to 5.0 ml w/feed tube Agar turn me on to it
That baby is the BOSS, when it comes to knocking up lots of jars and/or bags.
There are also GREAT for loading 10 ml spore syringes (with the proper fittings), from a bulk spore water jar.
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