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OfflineDelinquentes
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How much LC do you use?
    #5367556 - 03/05/06 02:19 PM (17 years, 10 months ago)

For those who use LC. How much of the white fluff needs to be in each injection site for the sample to be viable? Does there only need to be a speck? Do I need to make sure I have tons? I am gearing up the clean room to inoculate some popcorn I had prepared a few days ago. I only hope I am able to aspirate enough fluff in the smaller needles that I have.

Options and opinions welcome.


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"The price of meat has just gone up,
and your old lady has just gone down!" ~~ Zappa



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Offlinemycodetour
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Registered: 03/02/06
Posts: 136
Loc: WA
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Re: How much LC do you use? [Re: Delinquentes]
    #5367565 - 03/05/06 02:22 PM (17 years, 10 months ago)

i use as much as i can without messing up the water conent in the jars. i knocked up quart jars of rye berries with 10cc of lc and growth started in 24 hours.. the more you use, the quicker it colonizes.. but just a few flakes of myc should work if you are worried about running out


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my set up / log


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Offlinejoker_66599
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Re: How much LC do you use? [Re: Delinquentes]
    #5367570 - 03/05/06 02:24 PM (17 years, 10 months ago)

i think i was told more faster your jars will get going but i used 2-3cc a jar.

I just shook the hell out of the baby bottle and then sucked shit up.


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?¿
¿?
I don't really grow mushrooms I just act like it to be coo.
?¿
¿?


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OfflineDelinquentes
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Re: How much LC do you use? [Re: joker_66599]
    #5367591 - 03/05/06 02:31 PM (17 years, 10 months ago)

I will be taking this LC from my pint jar I posted pictures of. As of yet, I only have 2BB's with anything resembling mycelium.

I might need to wait it out a little and hope for more mycelium to be generated in the pint jar though.

Thanks!


--------------------
"The price of meat has just gone up,
and your old lady has just gone down!" ~~ Zappa



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Want to learn more about ozone as a bacteria killer?  Click Here


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Offlinejoker_66599
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Re: How much LC do you use? [Re: Delinquentes]
    #5367618 - 03/05/06 02:44 PM (17 years, 10 months ago)

i had 3 bb i used some out of 2 of them and th 3rd one is still sitting in my closet was the size of a hole in the middle of a cd not it's bigger than a golf ball took it about a month but grew huge


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?¿
¿?
I don't really grow mushrooms I just act like it to be coo.
?¿
¿?


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Invisibleagar
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Re: How much LC do you use? [Re: Delinquentes]
    #5367697 - 03/05/06 03:09 PM (17 years, 10 months ago)

One of the problems with an LC.

Is that it can grow into a thick mass, that will plug standard gauge needles.

The second problem is breaking up those myc clumps.

The best way to do that is with a magnetic stir plate & stir bar.


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OfflineDelinquentes
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Re: How much LC do you use? [Re: agar]
    #5367708 - 03/05/06 03:13 PM (17 years, 10 months ago)

Agar,

The stuff I have is very fine....especially since I have shaken it vigorously once a day. I think this stuff will suck up in to the needle, but won't know till the syringes I just PC'ed cool down.

I guess what I really wanted to know is this: Will the tiniest piece of mycelium be enough to get colonization going or do I need a syringe FULL of mycelium to get the job done?


--------------------
"The price of meat has just gone up,
and your old lady has just gone down!" ~~ Zappa



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Want to learn more about ozone as a bacteria killer?  Click Here


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Invisibleagar
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Re: How much LC do you use? [Re: Delinquentes]
    #5367756 - 03/05/06 03:26 PM (17 years, 10 months ago)

The more MYC particles in the syringe, THE BETTER.

Mine is near a slurry.

Try to get an LC as THICK, as possible.

If you only have a few small balls of myc fluff in a jar.

Try to suck those balls into a syringe.

Then, shake the HELL out of the syringe, to break them up even smaller.

The more myc particulates in a syringe, the more points of inoculation are created.

More contact points = THE BETTER.

Next time, try tubing filters on glass LC jars.
With GAS EXCHANGE, you will get thicker LC's.

The LC above, has a tiny hepa filter on the lid.
It was NEVER agitated, other than a mild swirl, once in awhile.


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OfflineDelinquentes
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Re: How much LC do you use? [Re: agar]
    #5367843 - 03/05/06 03:53 PM (17 years, 10 months ago)

I see.  Well, I have decided to wait it out a few days, and contiune to agitate the jar.  I am not in any real hurry, I was just bored :wink:

I guess I will build my fruiting chamber in preperation for my firt order of tennstudd goodness.

Thanks again, folks!


--------------------
"The price of meat has just gone up,
and your old lady has just gone down!" ~~ Zappa



Click Here for a ghetto ozone generator Tek.

Want to learn more about ozone as a bacteria killer?  Click Here


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OfflineSnaggletooth
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Re: How much LC do you use? [Re: Delinquentes]
    #5367895 - 03/05/06 04:06 PM (17 years, 10 months ago)

Here is a handy toy for LC  :grin:


Socorex  - 0.5 to 5.0 ml w/feed tube 


Agar turn me on to it:thumbup:


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Atheist Chat


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OfflineDelinquentes
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Re: How much LC do you use? [Re: Snaggletooth]
    #5367942 - 03/05/06 04:23 PM (17 years, 10 months ago)

Yeah, I like that beast. That will be when I take this to the next level. Once I have the opportunity to sample some of the fruits of may labor (pun intended) I am sure I am going to step up to the tools the professionals use. First thing will be a stir-plate and related equipment. I can use that as a multi-tasker for my other chemistry related endevors.


Snaggle - Can you PM me the details on that devil so I may locate and track one?


--------------------
"The price of meat has just gone up,
and your old lady has just gone down!" ~~ Zappa



Click Here for a ghetto ozone generator Tek.

Want to learn more about ozone as a bacteria killer?  Click Here


Edited by Delinquentes (03/05/06 04:26 PM)


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OfflineSnaggletooth
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Re: How much LC do you use? [Re: Delinquentes]
    #5367964 - 03/05/06 04:35 PM (17 years, 10 months ago)

Same here.......Soon after my first harvest I new I was going to be around for a while. :wink:

you can check this out Socorex - 0.5 to 5.0 ml w/feed tube

I still need to get a stirrer and stuff but toys are fun :grin:


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Atheist Chat


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Invisibleagar
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Re: How much LC do you use? [Re: Snaggletooth]
    #5368011 - 03/05/06 04:56 PM (17 years, 10 months ago)

Quote:

Snaggletooth said:
Here is a handy toy for LC  :grin:


Socorex  - 0.5 to 5.0 ml w/feed tube 
Agar turn me on to it:thumbup:




That baby is the BOSS, when it comes to knocking up lots of jars and/or bags.

There are also GREAT for loading 10 ml spore syringes (with the proper fittings), from a bulk spore water jar.


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