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Offlinemushsporeman
Stranger
Registered: 02/18/06
Posts: 28
Last seen: 7 years, 2 months
is my spore syringe contaminated?
    #5364937 - 03/04/06 03:24 PM (17 years, 10 months ago)

how can i tell if my spore syringe is contaminated?


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Offlinej21kickster
Stranger
Registered: 02/25/06
Posts: 69
Last seen: 17 years, 9 months
Re: is my spore syringe contaminated? [Re: mushsporeman]
    #5364953 - 03/04/06 03:33 PM (17 years, 10 months ago)

you cant really until you grow it out- the bacteria and mold spores are too small to see. What leads you to think it is contaminated?


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OfflineDelinquentes
TIMMMAYYYYYYY
Male User Gallery

Registered: 02/07/06
Posts: 543
Loc: Hidin' in a bowl of rice
Last seen: 3 months, 16 days
Re: is my spore syringe contaminated? [Re: j21kickster]
    #5364957 - 03/04/06 03:36 PM (17 years, 10 months ago)

Yes, what makes you think this? Do you have small white flecks in it? If so, this may be a good sign that your spores have germinated in situ. (in the syringe)

This seems to be an OK thing to have...just store it in the fridge from here on out.


--------------------
"The price of meat has just gone up,
and your old lady has just gone down!" ~~ Zappa



Click Here for a ghetto ozone generator Tek.

Want to learn more about ozone as a bacteria killer?  Click Here


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Offlinemushsporeman
Stranger
Registered: 02/18/06
Posts: 28
Last seen: 7 years, 2 months
Re: is my spore syringe contaminated? [Re: Delinquentes]
    #5365026 - 03/04/06 04:04 PM (17 years, 10 months ago)

well i lost the needle protector and looked around for it with the needle exposed and dropped the syringe on the floor picked it back up and flamed the needle with my lighter. also i used 2cc on each jar.(i think this is OK though i don't think 2cc per jar is bad as long as the substrate is not too wet and i don't think it was)
i knocked up 2 jars using the PF-tek 3 days ago....and i got this funny feeling that nothing will happen with them...i know this is not enough time yet and i know that after 5 days only a very small spec will start to grow and be visible. i have them in a shoe box at a temp of 75f 80f and humidity is at 32%. i have my top layer of verm to protect from contaminants with the foil wrapped tight in the shoe box.(when do i take the foil off?) i am pretty sure i did my mix right...i used 140ml verm and 40ml brown rice flour grounded from a coffee grinder then added my water and strained all excessive water.for some reason i just know nothing is going to happen.i sure hope i am wrong. am i doing this all right?


--------------------
May the wind always be at your back and the sun upon your face, and the winds of destiny carry you aloft to dance with the stars
* George Jung *


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Offlineshroomsynergy
Fungi Freak
Male

Registered: 01/17/06
Posts: 861
Loc: Andromeda
Last seen: 4 years, 4 months
Re: is my spore syringe contaminated? [Re: mushsporeman]
    #5365056 - 03/04/06 04:17 PM (17 years, 10 months ago)

i think that unless the syringe sucked up, (unlikely) you should be fine as long as you follow the guidelines and DONT DROP YOUR SYRINGES ANYMORE. good luck.


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Offlinemushsporeman
Stranger
Registered: 02/18/06
Posts: 28
Last seen: 7 years, 2 months
Re: is my spore syringe contaminated? [Re: mushsporeman]
    #5365060 - 03/04/06 04:19 PM (17 years, 10 months ago)

i should add i did not worry about any cleaning or contaminants while mixing my substrate. and i did let the jars cool in the pc for 8hours. i am only worried about being clean for contaminants when i open the PC after cooking them at which time i have everything ready go for the Inoculation.


--------------------
May the wind always be at your back and the sun upon your face, and the winds of destiny carry you aloft to dance with the stars
* George Jung *


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Offlinemushsporeman
Stranger
Registered: 02/18/06
Posts: 28
Last seen: 7 years, 2 months
Re: is my spore syringe contaminated? [Re: mushsporeman]
    #5365065 - 03/04/06 04:21 PM (17 years, 10 months ago)

when do i take the foil off?


--------------------
May the wind always be at your back and the sun upon your face, and the winds of destiny carry you aloft to dance with the stars
* George Jung *


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Offlinemushsporeman
Stranger
Registered: 02/18/06
Posts: 28
Last seen: 7 years, 2 months
Re: is my spore syringe contaminated? [Re: mushsporeman]
    #5365126 - 03/04/06 04:39 PM (17 years, 10 months ago)

someone?


--------------------
May the wind always be at your back and the sun upon your face, and the winds of destiny carry you aloft to dance with the stars
* George Jung *


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Invisiblecappa
Nerd
Registered: 02/12/06
Posts: 854
Re: is my spore syringe contaminated? [Re: mushsporeman]
    #5366263 - 03/05/06 12:41 AM (17 years, 10 months ago)

It doesn't really matter from what I can tell. From what I've read the foil is there to keep excess moisture from entering your jars while they are in the PC. You can probably remove the foil now, Although I don't see a harm in leaving loosely wrapped foil on the jars. 
:smile:


~Cappa.


--------------------
Their are 10 types of people. Those that understand binary, and those who don't.
~Cappa.


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Invisiblemusher_420
Stranger

Registered: 08/01/05
Posts: 2,691
Re: is my spore syringe contaminated? [Re: cappa]
    #5366283 - 03/05/06 12:57 AM (17 years, 10 months ago)

Dry verm acts as an excellent contam filter in the PF jars. The foil really isn't needed and will hinder the gas exchange in your jars.

I don't fully understand how you measured the water content for your jars. For next time use a mix of 2 parts Verm, one part BRF and one part water.

When doing open air inoculations keep an alcohol soaked paper towel wrapped around your needle in between inoculations.


--------------------
My Humidifier Reservoir Expansion Tek

Must read links for beginners

Btw- You are not what you own!


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