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Shop: Original Sensible Seeds Autoflowering Cannabis Seeds, Bulk Cannabis Seeds   North Spore Bulk Substrate, Cultivation Supplies

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OfflinekmIL
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Low Spawn:Substrate ratio... Any tips?
    #5364631 - 03/04/06 01:40 PM (17 years, 10 months ago)

My first attempt at making WBS spawn was kinda a failure and from what I was hoping to get 10 quarts I only got 2 :frown:  Do you old hands recommend using a 1:20 or even 1:30 spawn:substrate ratio?  Would this take SIGNIFICANTLY longer than say a 1:5 or 1:10?  Any tips on how I can avoid contams for this elongated colonization stage?  Thanks guys.


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Offlinejamman
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Re: Low Spawn:Substrate ratio... Any tips? [Re: kmIL]
    #5364637 - 03/04/06 01:42 PM (17 years, 10 months ago)

Just go for smaller amounts, 1:10 is as high as you should go. Best tip for avoiding contams is for quick colonization, which means lower ratios.


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Invisibleagar
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Re: Low Spawn:Substrate ratio... Any tips? [Re: kmIL]
    #5364650 - 03/04/06 01:48 PM (17 years, 10 months ago)

I would suggest 2 quarts of spawn to 7 or 8 quarts of substrate.

That will give you a fair sized tray.


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OfflineSnaggletooth
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Re: Low Spawn:Substrate ratio... Any tips? [Re: agar]
    #5364714 - 03/04/06 02:06 PM (17 years, 10 months ago)

The only thing I could add is make sure you mix the spawn

Thoroughly and evenly through out the substrate....and keep the moisture content correct


:bongload:


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InvisibleRoadkillM
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Re: Low Spawn:Substrate ratio... Any tips? [Re: jamman]
    #5364724 - 03/04/06 02:09 PM (17 years, 10 months ago)

Quote:

jamman said:

Just go for smaller amounts, 1:10 is as high as you should go.

Best tip for avoiding contams is for quick colonization, which means lower ratios.




:thumbup:


I always use the 5 to 1 ratio...myself.


tc


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OfflinekmIL
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Re: Low Spawn:Substrate ratio... Any tips? [Re: Roadkill]
    #5365544 - 03/04/06 07:17 PM (17 years, 10 months ago)

Thanks, but if I wanted to use a lower ratio, I could, right? Anybody know exactly how this affects colonization time? It would be an interesting experiment to see if it grows exponentially or linerally.


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Offlinesivad02487
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Re: Low Spawn:Substrate ratio... Any tips? [Re: kmIL]
    #5365554 - 03/04/06 07:21 PM (17 years, 10 months ago)

Well, if you kept EVERYTHING sterile, it may be possible. It will take a lot longer. Lower amount of spawn=longer waiting time and more chances for contams


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Invisiblemusher_420
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Re: Low Spawn:Substrate ratio... Any tips? [Re: kmIL]
    #5365585 - 03/04/06 07:31 PM (17 years, 10 months ago)

Quote:

kmIL said:
Thanks, but if I wanted to use a lower ratio, I could, right? Anybody know exactly how this affects colonization time? It would be an interesting experiment to see if it grows exponentially or linerally.





The way I see it. If you use half as much spawn, you have half as many inoculation points (assuming your applying the spawn the same way both times and conditions are the same.) and in theory it would take twice as long to colonize.

I would suggest taking extra steps for sterility. Wipe down all trays and materials with 70% isopropanol alcohol, cover gas exchange holes with micro-pore tape. Allow it to colonize inside of a clean box or something of the like.


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Edited by musher_420 (03/04/06 07:34 PM)


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Offlinesivad02487
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Re: Low Spawn:Substrate ratio... Any tips? [Re: musher_420]
    #5365618 - 03/04/06 07:46 PM (17 years, 10 months ago)

This guy knows what he's talking about. Keep everything sterile as fuck. Imagine trying to imitate a sterile laboratory environment


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OfflineChromeCrow
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Re: Low Spawn:Substrate ratio... Any tips? [Re: kmIL]
    #5365654 - 03/04/06 08:05 PM (17 years, 10 months ago)

first i would make 8 NEW jars and do a g2g.

if ya dont wanna go that route, i would grow in bags ( steralize everything ) this will give you a better chance to colonize more substrate in the added time your gonna get from using less spawn.

You could stretch the seed out to 3 or 4 bags per jar

8 full mycobags is a nice lil grow  :thumbup:


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InvisibleRahz
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Re: Low Spawn:Substrate ratio... Any tips? [Re: kmIL]
    #5365896 - 03/04/06 09:53 PM (17 years, 10 months ago)

Head to petsmart and grab a bag of crushed corn cob. Assuming your quart jars are 2/3 full (total of 1.33 quarts), scoop out 3-4 quarts of cob and place in a tub. Cover the cob with water and let it soak for 24 hours. Fill 2 pots half full with water and split the soaked cob between the two. Boil the cob for 20 minutes, keep an eye on it so it doesn't boil over (big mess).

If you have myco bags, use those, otherwise, while the cob is boiling, clean and sterilize the tub and lid. Once the cob is boiled, drain it (carefull not to get burned) and dump it in the tub. Secure the top and wait for it to cool to room temp (6-8 hours).

Once the cob is cooled, dump your colonized spawn in a bowl, break up well, and mix throughly with the cob. I add 2 quarts of hydrated cob to a myco bag, add 1 jar of spawn, and mix after sealing. If you use the tub, keep a cup of spawn to layer over the top. You'll want to keep your colonizing tub as sterile as possible. It would be a good idea to tape the lid up and add a 1 inch polyfil hole to the side. Another idea would be to use clean trashbags with a polyfil vent.

However you do it, let it sit for a week. Assuming you use 4 quarts of hydrated cob to your 1.33 quarts of spawn, you'll end up with 5.33 quarts (or 2/3 X 8) of spawn. This WORKS. I reciently ended up with way more spawn than I knew what to do with. I turned 7 jars into about 5 gallons of spawn using 2.5 quarts of cob per jar.

Rahz


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Offlinesivad02487
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Re: Low Spawn:Substrate ratio... Any tips? [Re: Rahz]
    #5365903 - 03/04/06 09:58 PM (17 years, 10 months ago)

You gotta remember that when doing any g2g transfers that it has to be ABSOLUTELY STERILE. I tried this in the middle of my kitchen and it fucked up horribly


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