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Offlinepcubmycol
flattiefisherman

Registered: 05/23/05
Posts: 747
Loc: Selectively breeding the ...
Last seen: 14 years, 3 months
Another tek from pcub - Simple Liquid Culture
    #5362788 - 03/03/06 10:05 PM (16 years, 5 months ago)

This teks permanant home is on my site's forums (gainesvilleps.org) here... Pcub's simple Liquid Culture

So i see alot of LC teks, most of them say PC... F*** that. LCs let alot of people down cause they just cant get it right... right? so here's my attempt to get the noobs more involved on what every grower knows is the faster method for grows.

Q. How does a liquid culture benefit me?
A. By eliminating your waiting for germination so read this.

What you'll need!

1. 3 1/2pt jars with bands and lids
2. Light Karo corn syrup
3. A pillow that has some poly-fil in it
4. an empty syringe (10 or 20ml)
5. a spore syringe
6. Some incubation chamber

The jar...
you'll need to make a quarter inch hole right near the edge of your jar, but not too close as to prevent the seal. Fill that hole with poly-fil so that the poly-fil is about 3 or 4 times as compressed as it is standing by itself.

The contents... (MUST BE EXACT)
You can use measuring cups and a teaspoon if you like, but it makes getting the right ratio alot harder. Syringes are cheap and easy to acquire at your local feed n seed shop or online at ryvmed.com

Measure out 55ml (55cc) of water and put it in your jar. Measure out 2ml of karo and put it in your jar. (I use 53ml to 2ml but 55 is an easier number because IMO a little under 4% is optimal although most say 4% is the way to go.)

Sterilization
Easy as pie... wrap double layered(or heavy duty) tin foil over top of jars half way down jar. put your jars in a pot with a rag on the bottom. fill with water up to water line in jar. Heat on high til boiling, reduce heat around med/low and cover. Let boil without opening lid (once you find the right point on your stove so that it doesnt boil over) for 20min. Let cool for 2hrs inside without opening top. If you go to long you get caramlized media (it turns yellowish-brown)... thats why we dont PC LCs, carmelization causes slowing in the growing...

inoculation
First things first, turn off the ac, heat, or fan. Wipe down all surfaces in your work area with lysol or some other sterilizer. nuke your whole kitchen with oust. No... really... nuke it. Now get out your styrofoam cooler, rubber gloves, and isopropyl alcohol. Cup your hand and pour a small amount of alcohol in one hand and rub your hands together as if you were washing them. The alcohol will evaporate within a few seconds. Remove your jars from the pot and place them in your work area but do not remove the foil yet. Very carefully remove the syringe from its bag but leave the needle cap on. Shake the syringe to mix up the spore water evenly. Remove the foil from your jars and tighten the lids. Remove the needle cap from your syringe. Stick the needle in an alcohol soaked paper towel and then in one of your jar's hole. eject 1/2cc in there. Do this as quickly as possible to every jar sticking the needle in the alcohol pad each time. Immediately after you have inoculated all the jars, put two coffee filters on top and rubber band them underneath the band. carefully place them into your cooler. YOUR DONE! If you do all of these steps and dont try to take shortcuts, you will not get ANY contamination!!

Incubate @ 84F

when theres a nickle sized blob, shake and you got 57 1/2cc of innoculant that skips the germination process in your jars.

Happy Trails and GL on your journeys
~pcubmycol


--------------------
weretheshit.com is the shit!

:-D


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OfflineDelinquentes
TIMMMAYYYYYYY
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Registered: 02/08/06
Posts: 537
Loc: Hidin' in a bowl of rice
Last seen: 1 year, 2 months
Re: Another tek from pcub - Simple Liquid Culture [Re: pcubmycol]
    #5362820 - 03/03/06 10:13 PM (16 years, 5 months ago)

Forgive me for being blunt, but there are several of these teks floating around. I'm not saying it's not good, but it's about the same as the ones available.

I appreciate you taking the time to simplify the process for all those apprehensive about trying it.

Again, I am not knocking the tek.


--------------------
"The price of meat has just gone up,
and your old lady has just gone down!" ~~ Zappa




Want to be the first on your block to have an ozone generator to kill contaminants?

Click Here for a ghetto ozone generator Tek.

Want to learn more about ozone as a bacteria killer?  Click Here


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InvisiblemushboyMDiscord
moooooooosh
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Registered: 04/24/05
Posts: 27,767
Loc: better trashcan
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Re: Another tek from pcub - Simple Liquid Culture [Re: pcubmycol]
    #5362832 - 03/03/06 10:16 PM (16 years, 5 months ago)

Besides ripping my pillow up this is a great tek. nicely written ferr of spaling eroars
5shrooms for you! :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2:


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Offlinepcubmycol
flattiefisherman

Registered: 05/23/05
Posts: 747
Loc: Selectively breeding the ...
Last seen: 14 years, 3 months
Re: Another tek from pcub - Simple Liquid Culture [Re: Delinquentes]
    #5362834 - 03/03/06 10:17 PM (16 years, 5 months ago)

well theres a million teks for everything... but thanks for the 8 minute bump.

I like to simplify things... it helps the newbs, and cause less questions for my brothers and I.

happy trails


--------------------
weretheshit.com is the shit!

:-D


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Offlinepcubmycol
flattiefisherman

Registered: 05/23/05
Posts: 747
Loc: Selectively breeding the ...
Last seen: 14 years, 3 months
Re: Another tek from pcub - Simple Liquid Culture [Re: pcubmycol]
    #5362844 - 03/03/06 10:19 PM (16 years, 5 months ago)

thanks mushboy... those 5 shrooms entitle you to one free print.. would you like.. (the current surplus is of KSSS)

or to wait for other beautiful microscopic viewing?


--------------------
weretheshit.com is the shit!

:-D


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OfflineDelinquentes
TIMMMAYYYYYYY
Male User Gallery

Registered: 02/08/06
Posts: 537
Loc: Hidin' in a bowl of rice
Last seen: 1 year, 2 months
Re: Another tek from pcub - Simple Liquid Culture [Re: pcubmycol]
    #5362847 - 03/03/06 10:21 PM (16 years, 5 months ago)

I don't mind bumping it.

I'm a noob and had to start somewhere. I got that start by following someone else's tek. That gave me the inspiration to search and that is when I realized there were more than one way to skin a cat.

If this post has inspired one person to go do that same search I did, then it has done it's job.


--------------------
"The price of meat has just gone up,
and your old lady has just gone down!" ~~ Zappa




Want to be the first on your block to have an ozone generator to kill contaminants?

Click Here for a ghetto ozone generator Tek.

Want to learn more about ozone as a bacteria killer?  Click Here


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Invisiblemskip23
Can It All Be So Simple!
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Registered: 06/08/05
Posts: 1,520
Loc: Philly
Re: Another tek from pcub - Simple Liquid Culture [Re: Delinquentes]
    #5362897 - 03/03/06 10:35 PM (16 years, 5 months ago)

I just contamd 40 quarts of wbs with an Lc that was contamd... :foreheadslap:
Nice seein ya pcub...btw I moved from the sunshine state  :thumbdown:


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url=https://files.shroomery.org/files/05-26/988356075-Picture-278.gif][/url


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Offlinepcubmycol
flattiefisherman

Registered: 05/23/05
Posts: 747
Loc: Selectively breeding the ...
Last seen: 14 years, 3 months
Re: Another tek from pcub - Simple Liquid Culture [Re: mskip23]
    #5362914 - 03/03/06 10:41 PM (16 years, 5 months ago)

nice seein you too brother... sorry to hear you left our mushroom capital.. Your god-like picture from the south is on GPS's main page. Ya can always still post there, your presence is missed..


Happy Trails...


--------------------
weretheshit.com is the shit!

:-D


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InvisibleTien
人民英雄
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Folding@home Statistics
Registered: 03/31/05
Posts: 2,382
Loc: Canoodia Flag
Re: Another tek from pcub - Simple Liquid Culture [Re: mskip23]
    #5362928 - 03/03/06 10:49 PM (16 years, 5 months ago)

Quote:

mskip23 said:
I just contamd 40 quarts of wbs with an Lc that was contamd... :foreheadslap:
Nice seein ya pcub...btw I moved from the sunshine state  :thumbdown:




dude...I thought I had a really healthy EQ LC but turned out to look so "healthy" because it was cobweb, I also found out the hard way. :nonono:

Pluto


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OfflineNumberThree
Jedi Drifter
Male

Registered: 02/05/06
Posts: 263
Last seen: 10 years, 7 months
Re: Another tek from pcub - Simple Liquid Culture [Re: Tien]
    #5362962 - 03/03/06 11:00 PM (16 years, 5 months ago)

Nice tek, I think I'm gonna go register on your site as well. The more knowledge at hand, the more power you can wield.


--------------------
Watch the weather change...



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Offlinepcubmycol
flattiefisherman

Registered: 05/23/05
Posts: 747
Loc: Selectively breeding the ...
Last seen: 14 years, 3 months
Re: Another tek from pcub - Simple Liquid Culture [Re: NumberThree]
    #5362975 - 03/03/06 11:05 PM (16 years, 5 months ago)

Glad to meet a new member to our community number three... Ill be glad to answer any Q's you have there on the forums.

and the more knowledge you can teach.

Happy Trails


--------------------
weretheshit.com is the shit!

:-D


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Offlinepcubmycol
flattiefisherman

Registered: 05/23/05
Posts: 747
Loc: Selectively breeding the ...
Last seen: 14 years, 3 months
Re: Another tek from pcub - Simple Liquid Culture [Re: pcubmycol]
    #5363871 - 03/04/06 10:07 AM (16 years, 5 months ago)

one last bump for the mornin since there's LC questions up there...

any other comments?

happy trails


--------------------
weretheshit.com is the shit!

:-D


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InvisibleTsinaglou
learning...
Male
Registered: 09/23/05
Posts: 79
Loc: midwest
Re: Another tek from pcub - Simple Liquid Culture [Re: pcubmycol]
    #5363936 - 03/04/06 10:51 AM (16 years, 5 months ago)

It's only recently I read that LC's if overcooked inhibit growth. That must explain my troubles, though some of the cubes seem able to tough it out. Recently I read that 15 minutes at 15 pounds pressure is good. Think that's too much?


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OfflineDelinquentes
TIMMMAYYYYYYY
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Posts: 537
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Re: Another tek from pcub - Simple Liquid Culture [Re: Tsinaglou]
    #5364067 - 03/04/06 11:53 AM (16 years, 5 months ago)

15-20 minutes should be fine.

I have cooked my Karo and my Honey for exactly 30 minutes after reaching 15psi. I have LC growing in at least the Karo. The honey is too young.


--------------------
"The price of meat has just gone up,
and your old lady has just gone down!" ~~ Zappa




Want to be the first on your block to have an ozone generator to kill contaminants?

Click Here for a ghetto ozone generator Tek.

Want to learn more about ozone as a bacteria killer?  Click Here


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OfflineOatman2000
-=Outa Space=-
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Registered: 05/10/05
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Re: Another tek from pcub - Simple Liquid Culture [Re: Tien]
    #5371382 - 03/06/06 06:01 PM (16 years, 5 months ago)

Quote:

Plutonium said:
Quote:

mskip23 said:
I just contamd 40 quarts of wbs with an Lc that was contamd... :foreheadslap:
Nice seein ya pcub...btw I moved from the sunshine state  :thumbdown:




dude...I thought I had a really healthy EQ LC but turned out to look so "healthy" because it was cobweb, I also found out the hard way. :nonono:

Pluto




I'm with ya brothers... actually contammed about 60-72 quarts with my bunk lc.  I kept thinking "it's gotta be the grain" unfortunaly it wasn't.... so, after comming out of a slup  "I'm back bitches"

Lc does contam fast like substrate will, and shows hardly any signs of doin soo which makes it dangerous for large batches.



Contam'd LC  (notice Pink on left)


--------------------
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:thumbup:  My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION
----------------------------

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