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Invisiblefigment
Apothecary
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Registered: 01/04/06
Posts: 522
Loc: Quidity
update on retarded first attempt
    #5359974 - 03/02/06 11:04 PM (17 years, 10 months ago)

I previously posted a problem I had with water content in cow dung/straw substrate.  They said I was doomed (among other things).

I cut open my three soaked spawn bags and mixed the contents with sterile perlite to get the correct moisture consistancy.  I was as sterile as I could be while mixing it.  Now it's incubating in the high 70's in a huge sterilte container.  I usually just inoculate the bags with spore, not a colonized medium and it usually works fine, so I am hoping that my spores germinate into myc. colonize before infection.

What do you guys think?  I am in the works on another plan, but I am just curious as to what you guys think about this "sure disaster" now. Will it work?  Is there even a small possibility? :crazy:


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Offlineg00n
Goombodias
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Registered: 08/19/05
Posts: 77
Loc: Sothern Cali!!!
Last seen: 16 years, 6 months
Re: update on retarded first attempt [Re: figment]
    #5359981 - 03/02/06 11:07 PM (17 years, 10 months ago)

There is always a small possibility. I have seen many certain dead jars come to life... if you have the time and space let it go.


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Offlinemycodetour
Mycologist inTraining

Registered: 03/02/06
Posts: 136
Loc: WA
Last seen: 17 years, 8 months
Re: update on retarded first attempt [Re: g00n]
    #5359990 - 03/02/06 11:11 PM (17 years, 10 months ago)

perlite? i think you wanted to use verm


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Invisiblefigment
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Registered: 01/04/06
Posts: 522
Loc: Quidity
Re: update on retarded first attempt [Re: mycodetour]
    #5360036 - 03/02/06 11:27 PM (17 years, 10 months ago)

I used perlite because that's what I had at the time. I just used it to get rid of all the excess water.


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Offlinerockytop83
Ornoth/Myc/ologist

Registered: 01/21/04
Posts: 588
Loc: Rocky-lachians
Last seen: 10 years, 6 months
Re: update on retarded first attempt [Re: figment]
    #5360054 - 03/02/06 11:33 PM (17 years, 10 months ago)

perlite in your spawn???

Perlite is used to raise humidity in the bottom of a FC. It doesnt have any nuts for the myc to feed on, nor will it hold moisture. Although this is mixed with Hpoo, I say its very unlikely this will work.


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Invisiblefigment
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Registered: 01/04/06
Posts: 522
Loc: Quidity
Re: update on retarded first attempt [Re: rockytop83]
    #5360139 - 03/02/06 11:58 PM (17 years, 10 months ago)

Let me clarify. I mixed perlite into my cow dung/straw mixture to get rid of the excess water. That's all. My main question is: will I see contamination before colonization?


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Offlinemycodetour
Mycologist inTraining

Registered: 03/02/06
Posts: 136
Loc: WA
Last seen: 17 years, 8 months
Re: update on retarded first attempt [Re: figment]
    #5360173 - 03/03/06 12:06 AM (17 years, 10 months ago)

so you made spawn bags with cpoo/straw/perlite and knocked them up with a spore syringe?


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OfflineDelinquentes
TIMMMAYYYYYYY
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Registered: 02/07/06
Posts: 543
Loc: Hidin' in a bowl of rice
Last seen: 3 months, 16 days
Re: update on retarded first attempt [Re: mycodetour]
    #5360255 - 03/03/06 12:32 AM (17 years, 10 months ago)

I have to disagee in a way...perlite WILL hold moisture. If it didn't my old ebb and flow system would not have worked as well as it did.

I can see some experimenting in my future.


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"The price of meat has just gone up,
and your old lady has just gone down!" ~~ Zappa



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