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figment
Apothecary


Registered: 01/04/06
Posts: 522
Loc: Quidity
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update on retarded first attempt
#5359974 - 03/02/06 11:04 PM (17 years, 10 months ago) |
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I previously posted a problem I had with water content in cow dung/straw substrate. They said I was doomed (among other things).
I cut open my three soaked spawn bags and mixed the contents with sterile perlite to get the correct moisture consistancy. I was as sterile as I could be while mixing it. Now it's incubating in the high 70's in a huge sterilte container. I usually just inoculate the bags with spore, not a colonized medium and it usually works fine, so I am hoping that my spores germinate into myc. colonize before infection.
What do you guys think? I am in the works on another plan, but I am just curious as to what you guys think about this "sure disaster" now. Will it work? Is there even a small possibility?
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g00n
Goombodias


Registered: 08/19/05
Posts: 77
Loc: Sothern Cali!!!
Last seen: 16 years, 6 months
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Re: update on retarded first attempt [Re: figment]
#5359981 - 03/02/06 11:07 PM (17 years, 10 months ago) |
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There is always a small possibility. I have seen many certain dead jars come to life... if you have the time and space let it go.
-------------------- I run with the hatchet and claim the PSYCHOPATHIC!!!
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mycodetour
Mycologist inTraining

Registered: 03/02/06
Posts: 136
Loc: WA
Last seen: 17 years, 8 months
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Re: update on retarded first attempt [Re: g00n]
#5359990 - 03/02/06 11:11 PM (17 years, 10 months ago) |
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perlite? i think you wanted to use verm
-------------------- my set up / log
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figment
Apothecary


Registered: 01/04/06
Posts: 522
Loc: Quidity
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Re: update on retarded first attempt [Re: mycodetour]
#5360036 - 03/02/06 11:27 PM (17 years, 10 months ago) |
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I used perlite because that's what I had at the time. I just used it to get rid of all the excess water.
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rockytop83
Ornoth/Myc/ologist

Registered: 01/21/04
Posts: 588
Loc: Rocky-lachians
Last seen: 10 years, 6 months
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Re: update on retarded first attempt [Re: figment]
#5360054 - 03/02/06 11:33 PM (17 years, 10 months ago) |
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perlite in your spawn???
Perlite is used to raise humidity in the bottom of a FC. It doesnt have any nuts for the myc to feed on, nor will it hold moisture. Although this is mixed with Hpoo, I say its very unlikely this will work.
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  ~You know that same rowdy crowd was here last night is back again~
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figment
Apothecary


Registered: 01/04/06
Posts: 522
Loc: Quidity
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Re: update on retarded first attempt [Re: rockytop83]
#5360139 - 03/02/06 11:58 PM (17 years, 10 months ago) |
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Let me clarify. I mixed perlite into my cow dung/straw mixture to get rid of the excess water. That's all. My main question is: will I see contamination before colonization?
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mycodetour
Mycologist inTraining

Registered: 03/02/06
Posts: 136
Loc: WA
Last seen: 17 years, 8 months
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Re: update on retarded first attempt [Re: figment]
#5360173 - 03/03/06 12:06 AM (17 years, 10 months ago) |
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so you made spawn bags with cpoo/straw/perlite and knocked them up with a spore syringe?
-------------------- my set up / log
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Delinquentes
TIMMMAYYYYYYY


Registered: 02/07/06
Posts: 543
Loc: Hidin' in a bowl of rice
Last seen: 3 months, 16 days
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Re: update on retarded first attempt [Re: mycodetour]
#5360255 - 03/03/06 12:32 AM (17 years, 10 months ago) |
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I have to disagee in a way...perlite WILL hold moisture. If it didn't my old ebb and flow system would not have worked as well as it did.
I can see some experimenting in my future.
-------------------- "The price of meat has just gone up, and your old lady has just gone down!" ~~ Zappa Click Here for a ghetto ozone generator Tek. Want to learn more about ozone as a bacteria killer? Click Here
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