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Offlinebuggers
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numerous species on agar and ??'s
    #5359693 - 03/02/06 09:45 PM (17 years, 10 months ago)

let me first say that anyone interested in this hobby should get into agar, it is one of the most satisfying aspect i have come across.
also, make a still air glovebox, my contam rates have dropped to next to nothing since i started using one.

my post:

i've been using my new glovebox a lot lately.
tonight i inoculated 20 agar dishes from fresh fruitbodies of 4 species.
1. P. Cubensis Tak mountain strain
2. White button mushroom
3. little portabellas
4. shitakes

i did 5 plates each. the white buttons, portabellas and shitakes came from a grocery store and the tak mountain came off of one of my caseings.

I've just been successful at getting colonized grain by cloning p. cubensis yangoon burmese strain on agar and then squirting sterile water from a syringe and muddling up the myc. on the dishes and then sucking the water back up and using that for innoculant. i've never worked with any of these other strains before.

will fresh storebought mushrooms work for cloning? will shitakes portabellas and white button mushrooms colonize Malt Dextrose Agar? will the myc if aspirated in a syringe as above work on grain?

my procedure was as follows. in still air glovebox with freshly poured peroxidated MDA plates i cut open each mushroom, cut five pieces out of the center of each stem or cap (being sure to avoid spore producing organs (gills)) allowed each piece to soak in h2o2 for a couple minutes before transferring to a plate.


what do you think? i figured i might save myself some $$ as a package of mushrooms at the store is like 2 bucks, i can eat them, AND i get free cultures with a little effort.

sorry if this post is a little disorganized i think i may have had a little too much to drink.


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OfflineRogerRabbitM
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Re: numerous species on agar and ??'s [Re: buggers]
    #5359726 - 03/02/06 09:57 PM (17 years, 10 months ago)

The problem with grocery store mushrooms is the farms expand their mycelium until the point of senesence before they fruit it. Some growers have reported success with store bought mushrooms, but personally my results have been dismal. They'll colonize just fine, but fruitings have been poor to not at all. Perhaps in different locals they don't expand the mycelium as much, but around me they do. Your tek is sound, but you can eliminate a step by simply slicing the agar into quarters and droping a quarter into each quart jar of grains. I'd recommend purchasing spawn from a reputable supplier rather than cloning.
RR


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Offlinebuggers
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Re: numerous species on agar and ??'s [Re: RogerRabbit]
    #5360977 - 03/03/06 09:11 AM (17 years, 10 months ago)

thanks for the info on on the possible inviability of my storebought strains.  do the commercial growers stretch substrains like this to the point of inviability simply because it is easier to repeatedly grow from tissue rather than starting from spores at intervals to ensure fresh genetics, or is it a means of preventing their own lab work from being extrapolated out of their fresh fruits by people like me (or more likely - their competition)?

hopefully i can get it to work but i'm not gonna cry if it doesnt.

i use the method of suspeniding mycelial tissue in water because it gives me lots of inoculation points rather than the single large one you get from wedges - i've just never had much luck with wedges.

thanks for your input. :thumbup:


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Offlinethenewguy05
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Re: numerous species on agar and ??'s [Re: RogerRabbit]
    #5360991 - 03/03/06 09:20 AM (17 years, 10 months ago)

Quote:

RogerRabbit said:
The problem with grocery store mushrooms is the farms expand their mycelium until the point of senesence before they fruit it. Some growers have reported success with store bought mushrooms, but personally my results have been dismal. They'll colonize just fine, but fruitings have been poor to not at all. Perhaps in different locals they don't expand the mycelium as much, but around me they do.
RR


this is true 100%. i've had success and i've had failure both using store bought mushrooms. in my area i have no idea what the hell they are doing over at that farm but sometimes you'll get a good batch and sometimes you get duds.


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Offlinebuggers
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Re: numerous species on agar and ??'s [Re: thenewguy05]
    #5363035 - 03/03/06 09:24 PM (17 years, 10 months ago)

mine came from P.A. i hear that 98 percent of edible mushrooms sold in usa come from P.a. so i'm guessing i'll have a problem with fruiting... any chance i could trade for some edible cultures?


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Offlinebuggers
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Re: numerous species on agar and ??'s [Re: buggers]
    #5363227 - 03/03/06 10:24 PM (17 years, 10 months ago)

admins please move to edibles or whatever. thx. ur kool.


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