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mikeownow
Humungus fungus

Registered: 09/01/05
Posts: 2,856
Loc: WA,USA
Last seen: 17 years, 3 months
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LC question
#5353840 - 03/01/06 03:00 PM (17 years, 10 months ago) |
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I did a honey liquid culter and used more than the recommended amount of honey. Anyway I pced it and there was just at tad bit of caramelized honey not much small small amount. Well now today there is stuff floating at the bottom and when I agitate the water it spreads and makes the water hard to see through. Is this myc or what?
for inoculation I used a peace from a brf cake. Is this just substrate from the cake floating around or can this happen in under 24 hours?
-------------------- No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.
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tiny_rabid_birds
Nocturnal


Registered: 11/08/05
Posts: 15,653
Loc: estados unidos
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Re: LC question [Re: mikeownow]
#5353866 - 03/01/06 03:09 PM (17 years, 10 months ago) |
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Yeah, growth can occur in 24 hours, especially since you inoculated with a live piece of myc rather than spores.
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rDr4g0n
Young Hand

Registered: 01/17/06
Posts: 587
Last seen: 17 years, 7 months
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when i did 2 of mine, both had the junk at the bottom. i wouldnt shake it becasue it would cloud up the water with the sediment. after a few days i saw blobs of white stuff that was obviously not sediment, so i knew it was working. id say let it sit a few days.
-------------------- i can speel... im just too lazy to sppelcheck. My first trip (good read) - Speed Leaching Poo! - My Second Trip (with art)
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mikeownow
Humungus fungus

Registered: 09/01/05
Posts: 2,856
Loc: WA,USA
Last seen: 17 years, 3 months
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Re: LC question [Re: rDr4g0n]
#5354795 - 03/01/06 07:59 PM (17 years, 10 months ago) |
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What do contaminants in a LC look like?
-------------------- No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.
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lardnar
Pu Pu Platter


Registered: 06/15/05
Posts: 703
Loc: Behind what appears
Last seen: 16 years, 11 months
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Re: LC question [Re: mikeownow]
#5354819 - 03/01/06 08:03 PM (17 years, 10 months ago) |
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I stopped bothering with carmelized cultures, sounds like you had the same problem I used to have - inoculate an LC with healthy liquid mycelium and watch it stall n sit at the bottom cause it's carmelized
-------------------- If your soul is sence this life is lost ...
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mikeownow
Humungus fungus

Registered: 09/01/05
Posts: 2,856
Loc: WA,USA
Last seen: 17 years, 3 months
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Re: LC question [Re: lardnar]
#5355936 - 03/02/06 12:04 AM (17 years, 10 months ago) |
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Quote:
lardnar said: I stopped bothering with carmelized cultures, sounds like you had the same problem I used to have - inoculate an LC with healthy liquid mycelium and watch it stall n sit at the bottom cause it's carmelized
I pced for only 25 minutes so it should not be carmelized realy.
-------------------- No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.
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mogur
regnartS

Registered: 11/15/05
Posts: 322
Loc: Puget Sound
Last seen: 11 years, 2 months
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Re: LC question [Re: mikeownow]
#5356048 - 03/02/06 12:51 AM (17 years, 10 months ago) |
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Quote:
mikeownow said:... I pced it and there was just at tad bit of caramelized honey not much small small amount.
Quote:
mikeownow said:... I pced for only 25 minutes so it should not be carmelized realy.
Some have claimed to have PC'd for 40 minutes without carmelization. I don't believe it. Often, I've seen people report carmelization at 20 minutes. You're not trying to kill endospores, here, just household bact and yeasts, so 15 mins is plenty. If you've ever made candy at home, you know that syrup will reach the hard ball stage at 250F. That is different, of course, because you are boiling the water off and concentrating the sugar, but the combo of time and temp will eventually break the sugars down. If you start with clear broth, it is WAY easier to tell contam from mycelium. Contams will cloud the broth and form cornmeal that settles to the bottom of the jar, or form off colored floating islands, or create a slimey film on the sides of the jar.
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mikeownow
Humungus fungus

Registered: 09/01/05
Posts: 2,856
Loc: WA,USA
Last seen: 17 years, 3 months
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Re: LC question [Re: mogur]
#5356138 - 03/02/06 01:33 AM (17 years, 10 months ago) |
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"Con tams will cloud the broth and form cornmeal that settles to the bottom of the jar,"
So the mycelium wont settle at the bottom because the stuff that looks like mycelium is settling at the bottom >.<
But when I shake it a little it spreads out all over.
-------------------- No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.
Edited by mikeownow (03/02/06 01:34 AM)
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mogur
regnartS

Registered: 11/15/05
Posts: 322
Loc: Puget Sound
Last seen: 11 years, 2 months
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Re: LC question [Re: mikeownow]
#5356241 - 03/02/06 02:53 AM (17 years, 10 months ago) |
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Mycelium pulls together into fluffy wispy clumps, often ball shaped, sometimes like a transparent jellyfish/mushroom shape. It also can be a whole bunch of little fluffy tufts, like really wet, large snowflakes. Myc can sometimes float at or near the top of the solution, but usually hangs at the bottom of the jar until agitated. And the solution stays clear.
I believe it's yeast contam when there's a murky layer of cornmeal or flour like sediment on the bottom of the jar. It will also 'spread out all over' when shaken, but it settles into a layer that has a distinct line between the sediment and liquid above. When myc is settled on the bottom, it is uneven and fluffy. If the entire solution gets so cloudy that you can no longer see anything through it, then I believe that may be bacteria contam.
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mikeownow
Humungus fungus

Registered: 09/01/05
Posts: 2,856
Loc: WA,USA
Last seen: 17 years, 3 months
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Re: LC question [Re: mogur]
#5360264 - 03/03/06 12:35 AM (17 years, 10 months ago) |
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Well since the mycelium is sinking I am now doing a test. I made a norther LC but did not PC it and did not add any mycelium to it. I took a knife and stabbed the same cake so that any spores or germs from the outside of the cake would get onto the knife and I scraped it on the side of the jar to get off any micro sized mycelium off and then stuck the knife in the honey water. I did this in the same bathroom that I did the other expert I did not do any oust or bleach water spray. O made sure to blow into it to make sure lots of germ things that may be in the bathroom get in.
Now if this 2nd jar does the same thing as the pced mycelium one then I know that the myc one is bad. If it stays the same I will inoculate all 7 of my grain quart jars.
Edit: all coments are apreciated.
-------------------- No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.
Edited by mikeownow (03/03/06 12:36 AM)
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Delusionz
<( '.' )>


Registered: 02/20/06
Posts: 89
Loc: U.S. West
Last seen: 16 years, 10 months
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Re: LC question [Re: mikeownow]
#5360318 - 03/03/06 12:59 AM (17 years, 10 months ago) |
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why dont you just do another clean jar, PC for only 15 min, try and do it in a more sterile condition, and see if comes out better?
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mikeownow
Humungus fungus

Registered: 09/01/05
Posts: 2,856
Loc: WA,USA
Last seen: 17 years, 3 months
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Re: LC question [Re: Delusionz]
#5360342 - 03/03/06 01:10 AM (17 years, 10 months ago) |
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I did it as sterile as I could. Ousted the air let settle then aired room out with bathroom fan then 3 caps of bleach in a spray bottle and sprayed around the room let settle then turned fan on and went to work.
-------------------- No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.
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mikeownow
Humungus fungus

Registered: 09/01/05
Posts: 2,856
Loc: WA,USA
Last seen: 17 years, 3 months
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Re: LC question [Re: mikeownow]
#5360348 - 03/03/06 01:12 AM (17 years, 10 months ago) |
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Maybe this is happening the way it is because I used so much honey lol. What is the consequence for using to much honey?
-------------------- No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.
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lardnar
Pu Pu Platter


Registered: 06/15/05
Posts: 703
Loc: Behind what appears
Last seen: 16 years, 11 months
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Re: LC question [Re: mikeownow]
#5366302 - 03/05/06 01:04 AM (17 years, 10 months ago) |
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Quote:
mikeownow said:
I pced for only 25 minutes so it should not be carmelized realy.
Mine carmelize at 20 minutes. Searching the old posts I found someone who was having problems at 15 minutes. Im now trying steaming the jars instead, hoping it works well
-------------------- If your soul is sence this life is lost ...
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Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
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Re: LC question [Re: lardnar]
#5366574 - 03/05/06 06:40 AM (17 years, 10 months ago) |
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no need to PC at all, simple boiling temperature is adequate
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
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Re: LC question [Re: Hippie3]
#5366798 - 03/05/06 09:27 AM (17 years, 10 months ago) |
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Quote:
Hippie3 said: no need to PC at all, simple boiling temperature is adequate
I agree also. 5 minutes minimum at a rolling boil.
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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mogur
regnartS

Registered: 11/15/05
Posts: 322
Loc: Puget Sound
Last seen: 11 years, 2 months
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Re: LC question [Re: mikeownow]
#5366938 - 03/05/06 10:16 AM (17 years, 10 months ago) |
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Quote:
mikeownow said: ... then turned fan on and went to work.
Shouldn't work with the fan on. All sources of air movement need to be off for as long as possible, say a half hour or so. The important thing to remember is that dust always falls in still air. Given a chance to settle, the bad guys will be sitting on the floor and countertops. Disinfecting the work surface, and minimizing air turbulence caused by your movements, greatly reduces the chance that a contam can waft its way onto your work. Flow hoods, of course, are much different, and use the movement of clean air to exclude contams.
PS Think of a scuba diver trying to work where the bottom is very silty. Any turbulence will be obvious and eventually obscure his vision. Dust in the air isn't so easy to visualize, but it acts a lot like silt does in water.
Edited by mogur (03/05/06 10:32 AM)
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
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Re: LC question [Re: mogur]
#5367087 - 03/05/06 11:15 AM (17 years, 10 months ago) |
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Quote:
mogur said: PS Think of a scuba diver trying to work where the bottom is very silty. Any turbulence will be obvious and eventually obscure his vision. Dust in the air isn't so easy to visualize, but it acts a lot like silt does in water.
Great analogy!
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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