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InvisibleTODAY
Battletoad
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Registered: 09/25/03
Posts: 10,218
Loc: Metropolis City, USA
Don't let me fuck up bulk again!!
    #5349337 - 02/28/06 01:14 PM (17 years, 10 months ago)

I have a layer of dry, shredded straw in a container 16"x10" and 4" thick.  I haven't added water but plan on oven bag pasturizing as soon as the experienced folks have OK'd my method.  The problem is, I only have 1 quart of WBS spawned to use as spawn.  Is this enough? I originally wanted to use 2 quarts and would have been happy with that but the mold god decided to make it otherwise  :shocked:.

I just want to know, how long am I going to be waiting for my straw to be colonized with only 1 quart of spawn?  I know time is of the essence concerning any spawn run, especially straw, if you don't want contamination.  :thumbup:


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ca'rouse (k-rouz)
intr.v.
To engage in boisterous, drunken merrymaking.


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OfflinerDr4g0n
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Registered: 01/17/06
Posts: 587
Last seen: 17 years, 7 months
Re: Don't let me fuck up bulk again!! [Re: TODAY]
    #5349347 - 02/28/06 01:16 PM (17 years, 10 months ago)

put the quart you have in the fridge and wait for another jar to colonize. you can do a grain to grain transfer to get the other quart colonized faster.


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i can speel... im just too lazy to sppelcheck.

My first trip (good read) - Speed Leaching Poo! - My Second Trip (with art)


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Offlinethenewguy05
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Re: Don't let me fuck up bulk again!! [Re: rDr4g0n]
    #5349414 - 02/28/06 01:29 PM (17 years, 10 months ago)

well you have a few options. take away some straw or grain transfer to make more spawn.

grain to grain is the way IMO. what i do is take one jar and turn it into 6 jars. paul stamets says you can do 10 but i need faster colonization speed so i do 6.


Edited by thenewguy05 (02/28/06 01:31 PM)


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InvisibleTODAY
Battletoad
Male

Registered: 09/25/03
Posts: 10,218
Loc: Metropolis City, USA
Re: Don't let me fuck up bulk again!! [Re: thenewguy05]
    #5349428 - 02/28/06 01:32 PM (17 years, 10 months ago)

You two both suggest to wait for more spawn then.  I suppose a G2G would only push back the project a week and a half or two.  That should be fine :smile:.  Thanks!


--------------------

ca'rouse (k-rouz)
intr.v.
To engage in boisterous, drunken merrymaking.


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Offlinethenewguy05
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Re: Don't let me fuck up bulk again!! [Re: TODAY]
    #5349447 - 02/28/06 01:37 PM (17 years, 10 months ago)

prolly only a week and a half at most. live mycelium colonizes faster than spores do. you've made a wise decision.


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OfflineMitchnast
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Registered: 10/27/99
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Re: Don't let me fuck up bulk again!! [Re: thenewguy05]
    #5349519 - 02/28/06 01:53 PM (17 years, 10 months ago)

yeah, to put it bluntly, once you get a viable strain going, even at the end of a project, you can just take a colonized hunk of grain or straw or even mushroom tissue, give it a dip in peroxide, and pop it onto agar.
you dont even have to be particularly sterile, just give it a spray of peroxide evry so often and mold and bacteria wont take hold. Ive gotten agar to fully colonize this way even after it contaminated.

if you have viable myc, then youre laughing. It's so cleanable. and if its already established, it will outrun most contams.
after a while you dont even have to bother with spores and innoculating.

just keep the strain going, the purer the strain gets, the less it competes. that turns into bigger harvests and greater resistance to contam.


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InvisibleTODAY
Battletoad
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Registered: 09/25/03
Posts: 10,218
Loc: Metropolis City, USA
Re: Don't let me fuck up bulk again!! [Re: Mitchnast]
    #5350683 - 02/28/06 07:08 PM (17 years, 10 months ago)

thanks, that's good stuff to know mitchnast. and newuser, i could do the G2G but i'd have to buy more jars because i'm out of those.

On another note, i just found about 1-1.5 viable pints of fatass colonizing that I had given for dead because I thought it was too dry to colonize.

This means that I have:
1 pint Puerto Rican
1 pint Pink Buffalo
1-1.5 pints Fatass

Should I go for it and mix this into the pasteurized straw? 3 different strains may be a bad idea to mix in a bulk grow but if they are still able to independently colonize the straw, why should I care if one strain is able to outcolonize/outfruit the other strains? I'm not making this batch for printing purposes so is there any reason I shouldn't just give this a try?


--------------------

ca'rouse (k-rouz)
intr.v.
To engage in boisterous, drunken merrymaking.


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