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OfflineShimen
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Bunk Spores???
    #5348896 - 02/28/06 11:08 AM (17 years, 10 months ago)

Hey guys got a question. I am on my second attempt to grow with BRF cakes. I ordered my sryinge from spores101 and well i have had no growth. The new jars have been inoc'ed about 3 days ago. I know that that is not longth enough to start making assumptions but, I also have a LC (honeywater) that i knocked up about a week ago. I see growth in it but hardly any at all. So whats the deal here. I followed instructions to the T. Am i just doing something wrong. Its getting me frustrated. Thanks for any help you can offer. OH BTW my strain is Mexi-Cube.  :minigun:


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Death is when you become bored with life.


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OfflineWronguy
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Re: Bunk Spores??? [Re: Shimen]
    #5348907 - 02/28/06 11:12 AM (17 years, 10 months ago)

What is your LC in, a baby bottle, a mason jar? How are you providing gas exchange to your LC?


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OfflineShimen
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Re: Bunk Spores??? [Re: Wronguy]
    #5348914 - 02/28/06 11:14 AM (17 years, 10 months ago)

My LC is in a Ball Mason Jar. I has layer of Tyvek then a polyfil filter hole in it. i got told that provides anough gas exchange. and its just one hole. about 1/8 inch in diameter.


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Death is when you become bored with life.


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Offlinestrangladesh
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Re: Bunk Spores??? [Re: Wronguy]
    #5348915 - 02/28/06 11:14 AM (17 years, 10 months ago)

ive had a bad experiance with this company, not their cubie spores thogh those worked fine.


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OfflineShimen
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Re: Bunk Spores??? [Re: strangladesh]
    #5348928 - 02/28/06 11:19 AM (17 years, 10 months ago)

Quote:

strangladesh said:
ive had a bad experiance with this company, not their cubie spores thogh those worked fine.




So should i just order some more spores. I was thinking if i did to go through ralphsters this time. I read good things about him on this site.


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OfflineWronguy
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Re: Bunk Spores??? [Re: Shimen]
    #5348931 - 02/28/06 11:20 AM (17 years, 10 months ago)

In my experience the hole needs to be a minimum of 1/2" to be of any use. I don't think you need Tyvek and poly-fill, seems a bit redundant to me. Make sure you're agitating the LC everyday for optimal results. If you have the means, I would make an effort to increase the size of the hole and agitate everyday.

Most liquid culture failures can be attributed to improper gas exchange and lack of agitation. The other substrate you inoculated sounds normal to me. Spore germination can easily take 3-5 days and is not unusual to take 7-10. Use a flashlight to look for mycelium in your LC. Sometimes they're there, just harder to see without proper lighting.


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OfflineShimen
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Re: Bunk Spores??? [Re: Wronguy]
    #5348942 - 02/28/06 11:24 AM (17 years, 10 months ago)

Alright i will make a bigger hole. Would it be ok to take the lid off. And should i use tyvek still and just eliminate the polyfil or neither.


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OfflineWronguy
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Re: Bunk Spores??? [Re: Shimen]
    #5348977 - 02/28/06 11:34 AM (17 years, 10 months ago)

You can take the lid off in a sterile glove box. NEVER take the lid off any substrate in open air. You'll catch a contaminate quicker than you can spit.

Using Tyvek or poly-fill will suffice. I personally prefer poly-fill but its up to you.


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OfflineShimen
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Re: Bunk Spores??? [Re: Wronguy]
    #5349017 - 02/28/06 11:45 AM (17 years, 10 months ago)

Hey could i just take the inner lid off and leave the ring and tyvek filter on


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Offlinehyphae
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Re: Bunk Spores??? [Re: Wronguy]
    #5349021 - 02/28/06 11:45 AM (17 years, 10 months ago)

My first question would be what are your incubating temps at? Because they should be in the low to mid 80's also don't forget to shake the syringes although a couple of spore sites use ultrasonics and a suspension agent so shaking is no longer necessary. :wink:GL


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Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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InvisibleRoadkillM
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Re: Bunk Spores??? [Re: Wronguy]
    #5349023 - 02/28/06 11:46 AM (17 years, 10 months ago)

Quote:

Wronguy said:

Most liquid culture failures can be attributed to improper gas exchange.

Spore germination can easily take 3-5 days and is not unusual to take 7-10.




:thumbup:


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You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineShimen
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Re: Bunk Spores??? [Re: Roadkill]
    #5349050 - 02/28/06 11:54 AM (17 years, 10 months ago)

I got a small TiT holds 6 pints jars. (Wanted to start off small) and yes i keep it at about 83 in there. and another yes, i did shake the syringe. I hope this works because i dont want to buy another syringe. I want to make my own. Do my BRF jars need to have a big hole in them also for gas exchange?


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Death is when you become bored with life.


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OfflineWronguy
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Re: Bunk Spores??? [Re: Shimen]
    #5349094 - 02/28/06 12:06 PM (17 years, 10 months ago)

The holes for your inoculation points will provide enough gas exchange. You should have 4-5 in there, which together will provide what you need.


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Re: Bunk Spores??? [Re: Wronguy]
    #5349108 - 02/28/06 12:09 PM (17 years, 10 months ago)

even if the hole is small like 1/8 inch in diameter. Also why couldn't you just leave the inner lid off and use a tyvek lid. then seal the inoc holes up with micropore or something, or would that provide to much exchange and dry out the cakes.


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OfflineWronguy
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Re: Bunk Spores??? [Re: Shimen]
    #5349156 - 02/28/06 12:22 PM (17 years, 10 months ago)

If you have multiple inoculation holes they will equal the appropriate amount of air flow needed when combined. You don't want to just have a Tyvek lid for an opening. You'll experience excessive evaporation of moisture and dry your substrate out. The inner lid is required.


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OfflineMaverick
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Re: Bunk Spores??? [Re: Wronguy]
    #5349158 - 02/28/06 12:24 PM (17 years, 10 months ago)

I had the same problem with Spores101.  Bunk spores.  Everything went smooth, but not a single bit of growth, not even bacterial. :frown:


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OfflineShimen
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Re: Bunk Spores??? [Re: Maverick]
    #5349190 - 02/28/06 12:31 PM (17 years, 10 months ago)

Alright that is what i was thinking cool. THanks alot for all your help.... I will keep you guys updated.


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