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Offlineweezle
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substrate sterilization
    #5348326 - 02/28/06 05:14 AM (17 years, 10 months ago)

alright i have an aluminum baking sheet of substrate..... i dont know how to sterilize it.. but i can pull just about anything thats suggested... any help? well im thinking of baking it for 3 hours but i dont get any help here ever anyway thanks guys.... no help for the new guy.... why do i even come here i should jump off a bridge get things done alot quicker that way....


Edited by weezle (02/28/06 05:35 AM)


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InvisibleFooMan
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Re: substrate sterilization [Re: weezle]
    #5348342 - 02/28/06 05:38 AM (17 years, 10 months ago)

What's the substrate? Most will dry out if baked.


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Offlineweezle
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Re: substrate sterilization [Re: FooMan]
    #5348343 - 02/28/06 05:38 AM (17 years, 10 months ago)

a little vermucilite brown rice flour and some birdseed enough for 12 jars...


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InvisibleFooMan
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Re: substrate sterilization [Re: weezle]
    #5348345 - 02/28/06 05:38 AM (17 years, 10 months ago)

You can also scroll down to "related threads" at the bottom of this page for answers.


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InvisibleFooMan
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Re: substrate sterilization [Re: weezle]
    #5348347 - 02/28/06 05:40 AM (17 years, 10 months ago)

Quote:

weezle said:
a little vermucilite brown rice flour and some birdseed enough for 12 jars...




Get a PC or get rid of the bird seed and boil the jars for an hour or two.


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Offlineweezle
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Re: substrate sterilization [Re: FooMan]
    #5348352 - 02/28/06 05:42 AM (17 years, 10 months ago)

ive boiled a hundred times....... and every single time its the same contam after contam im sick of green ickyness killing my failed attempts and ive boiled for hours... tried a thousand different things...


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OfflineRogerRabbitM
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Re: substrate sterilization [Re: weezle]
    #5348357 - 02/28/06 05:48 AM (17 years, 10 months ago)

Green molds are killed by temperatures far below boiling. If you're getting trichoderma, it's being introduced after your sterilization process. We PC to kill bacterial endospores, not molds.
RR


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Offlineweezle
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Re: substrate sterilization [Re: RogerRabbit]
    #5348358 - 02/28/06 05:51 AM (17 years, 10 months ago)

yeah but i aint got a pressure cooker.... i dont know but this is getting kind of stupid i think im gonna go my own way and see if i can figure something out unless someone can hit me with some solid way of santizing a substrate in a baking pan...


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InvisibleFooMan
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Re: substrate sterilization [Re: weezle]
    #5348372 - 02/28/06 05:59 AM (17 years, 10 months ago)

If you're using bird seed in your mix and boiling, you WILL get contams. Boiling doesn't get the temp up high enough to kill the endospores in the grains.

If you are boiling and not PC'ing, you should only be using BRF and verm.


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OfflineRogerRabbitM
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Re: substrate sterilization [Re: FooMan]
    #5348401 - 02/28/06 06:28 AM (17 years, 10 months ago)

You CAN'T sterilize grains in a baking pan, no matter what. It's physically impossible for moisture to exist in a liquid state above 212F/100C. Once your grains reach that temperature in the oven, they will be as dry as when you took them out of the bag from the store.
RR


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OfflineDelinquentes
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Re: substrate sterilization [Re: RogerRabbit]
    #5348417 - 02/28/06 06:56 AM (17 years, 10 months ago)

Not to mention the fact that transfering the substrate to the jars after sterilization is going to introduce contaminants. I know I have read here about how to do the PF tek without a PC. Perhaps you should be folowwing that method. It has worked for others.


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Offlineweezle
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Re: substrate sterilization [Re: RogerRabbit]
    #5348427 - 02/28/06 07:06 AM (17 years, 10 months ago)

well i went and baked it for 3 hours anyway.... then i threw it in ziplock bags microwaved them for 2 minutes 3 times each then remicrod them in their containers for 2 minutes... is it possible to work or am i destined to failure due to poverty?


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OfflineNewbieS
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Re: substrate sterilization [Re: weezle]
    #5348573 - 02/28/06 08:33 AM (17 years, 10 months ago)

Just put some money away for a good PC. You know you want one...you're thinking about it right now; all shiney, and...pressure-cooker-y. You can't (shouldn't) do grains without it so you make a choice: Mold or Mushrooms? ;-)


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Re: substrate sterilization [Re: weezle]
    #5348643 - 02/28/06 09:09 AM (17 years, 10 months ago)

Quote:

weezle said:
is it possible to work or am i destined to failure due to poverty?




It's possible, but doubtful that it will work. You aren't failing because of poverty. I think it's because you aren't following directions or studying up on this. You boil or PC instead of baking to retain the substrate's moisture content. You don't use WBS in your mix if you don't have a PC, otherwise you'll have to do fractional sterilization. If you follow the tek TO A "T", you shouldn't have any problems.

I'm still wondering why you have WBS in your cake mix :confused:


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Offlinecoda
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Re: substrate sterilization [Re: FooMan]
    #5348791 - 02/28/06 10:27 AM (17 years, 10 months ago)

im wondering why this guy bothered all of you then ignored all your sound advice.


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i really am glad you came back to us instead of taking the other path. *hug*

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