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weezle
Stranger
Registered: 03/13/04
Posts: 13
Last seen: 17 years, 10 months
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substrate sterilization
#5348326 - 02/28/06 05:14 AM (17 years, 10 months ago) |
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alright i have an aluminum baking sheet of substrate..... i dont know how to sterilize it.. but i can pull just about anything thats suggested... any help? well im thinking of baking it for 3 hours but i dont get any help here ever anyway thanks guys.... no help for the new guy.... why do i even come here i should jump off a bridge get things done alot quicker that way....
Edited by weezle (02/28/06 05:35 AM)
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: substrate sterilization [Re: weezle]
#5348342 - 02/28/06 05:38 AM (17 years, 10 months ago) |
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What's the substrate? Most will dry out if baked.
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weezle
Stranger
Registered: 03/13/04
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Last seen: 17 years, 10 months
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Re: substrate sterilization [Re: FooMan]
#5348343 - 02/28/06 05:38 AM (17 years, 10 months ago) |
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a little vermucilite brown rice flour and some birdseed enough for 12 jars...
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: substrate sterilization [Re: weezle]
#5348345 - 02/28/06 05:38 AM (17 years, 10 months ago) |
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You can also scroll down to "related threads" at the bottom of this page for answers.
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Quick WBS Prep
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: substrate sterilization [Re: weezle]
#5348347 - 02/28/06 05:40 AM (17 years, 10 months ago) |
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Quote:
weezle said: a little vermucilite brown rice flour and some birdseed enough for 12 jars...
Get a PC or get rid of the bird seed and boil the jars for an hour or two.
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Quick WBS Prep
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weezle
Stranger
Registered: 03/13/04
Posts: 13
Last seen: 17 years, 10 months
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Re: substrate sterilization [Re: FooMan]
#5348352 - 02/28/06 05:42 AM (17 years, 10 months ago) |
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ive boiled a hundred times....... and every single time its the same contam after contam im sick of green ickyness killing my failed attempts and ive boiled for hours... tried a thousand different things...
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: substrate sterilization [Re: weezle]
#5348357 - 02/28/06 05:48 AM (17 years, 10 months ago) |
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Green molds are killed by temperatures far below boiling. If you're getting trichoderma, it's being introduced after your sterilization process. We PC to kill bacterial endospores, not molds. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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weezle
Stranger
Registered: 03/13/04
Posts: 13
Last seen: 17 years, 10 months
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Re: substrate sterilization [Re: RogerRabbit]
#5348358 - 02/28/06 05:51 AM (17 years, 10 months ago) |
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yeah but i aint got a pressure cooker.... i dont know but this is getting kind of stupid i think im gonna go my own way and see if i can figure something out unless someone can hit me with some solid way of santizing a substrate in a baking pan...
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: substrate sterilization [Re: weezle]
#5348372 - 02/28/06 05:59 AM (17 years, 10 months ago) |
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If you're using bird seed in your mix and boiling, you WILL get contams. Boiling doesn't get the temp up high enough to kill the endospores in the grains.
If you are boiling and not PC'ing, you should only be using BRF and verm.
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
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Re: substrate sterilization [Re: FooMan]
#5348401 - 02/28/06 06:28 AM (17 years, 10 months ago) |
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You CAN'T sterilize grains in a baking pan, no matter what. It's physically impossible for moisture to exist in a liquid state above 212F/100C. Once your grains reach that temperature in the oven, they will be as dry as when you took them out of the bag from the store. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Delinquentes
TIMMMAYYYYYYY


Registered: 02/07/06
Posts: 543
Loc: Hidin' in a bowl of rice
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Re: substrate sterilization [Re: RogerRabbit]
#5348417 - 02/28/06 06:56 AM (17 years, 10 months ago) |
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Not to mention the fact that transfering the substrate to the jars after sterilization is going to introduce contaminants. I know I have read here about how to do the PF tek without a PC. Perhaps you should be folowwing that method. It has worked for others.
-------------------- "The price of meat has just gone up, and your old lady has just gone down!" ~~ Zappa Click Here for a ghetto ozone generator Tek. Want to learn more about ozone as a bacteria killer? Click Here
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weezle
Stranger
Registered: 03/13/04
Posts: 13
Last seen: 17 years, 10 months
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Re: substrate sterilization [Re: RogerRabbit]
#5348427 - 02/28/06 07:06 AM (17 years, 10 months ago) |
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well i went and baked it for 3 hours anyway.... then i threw it in ziplock bags microwaved them for 2 minutes 3 times each then remicrod them in their containers for 2 minutes... is it possible to work or am i destined to failure due to poverty?
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Newbie
User of semicolons.


Registered: 07/18/04
Posts: 24,710
Loc: SoCal
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Re: substrate sterilization [Re: weezle]
#5348573 - 02/28/06 08:33 AM (17 years, 10 months ago) |
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Just put some money away for a good PC. You know you want one...you're thinking about it right now; all shiney, and...pressure-cooker-y. You can't (shouldn't) do grains without it so you make a choice: Mold or Mushrooms? ;-)
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: substrate sterilization [Re: weezle]
#5348643 - 02/28/06 09:09 AM (17 years, 10 months ago) |
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Quote:
weezle said: is it possible to work or am i destined to failure due to poverty?
It's possible, but doubtful that it will work. You aren't failing because of poverty. I think it's because you aren't following directions or studying up on this. You boil or PC instead of baking to retain the substrate's moisture content. You don't use WBS in your mix if you don't have a PC, otherwise you'll have to do fractional sterilization. If you follow the tek TO A "T", you shouldn't have any problems.
I'm still wondering why you have WBS in your cake mix
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coda
Banjo Goiter


Registered: 03/20/01
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Re: substrate sterilization [Re: FooMan]
#5348791 - 02/28/06 10:27 AM (17 years, 10 months ago) |
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im wondering why this guy bothered all of you then ignored all your sound advice.
-------------------- To get really high is to forget yourself. And to forget yourself is to see everything else. And to see everything else is to become an understanding molecule in evolution, a conscious tool of the universe. And I think every human being should be a conscious tool of the universe. . . . -JG i really am glad you came back to us instead of taking the other path. *hug* -A_S (RIP your final words to me will never be forgotten)
 Don't fuck with the laughing jesus.
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