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SHA2
Stranger

Registered: 02/23/06
Posts: 23
Last seen: 17 years, 7 months
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whats going on? (INCUBATION stage)
#5348289 - 02/28/06 04:37 AM (17 years, 10 months ago) |
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Hey guyz, its been 3 days after incubation and there is no sign of colonizing wutsoever. (at 84 - 86 f)
this is my first time grwoing mushrooms. everything was ok till i wanted to do sterilizing the jars. I dont have a PC so i used normal boiling pots. boiled them with a lid on so steam would help to sterilize the jars better. I did more than 1:30 and 3 of my jars broke so i through them away.
the other 3 were ok. so after 1 hour when they were pretty cooled down i injected the spores (using a syringe). Now I cant see any sign of ocolonizing and was thinking may be something is wrong.
reasons which you may agree: 1- overcooked the jars 2- didnt wait enough till the jars cool down 3- should wait more to see the results
ow and btw my jars are 500 ml (pint) and i injected about 1.2 ml in each.
any ideas?
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lardnar
Pu Pu Platter


Registered: 06/15/05
Posts: 703
Loc: Behind what appears
Last seen: 16 years, 11 months
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Re: whats going on? (INCUBATION stage) [Re: SHA2]
#5348333 - 02/28/06 05:24 AM (17 years, 10 months ago) |
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You should have waited for the jars to cool down, but 3 days isn't long enough to worry about not seeing growth. I would be worried about the jars still being too hot though, and about sterilizing larger than 1/2 pint jars with boiling
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Woland
The MercilessWarrior


Registered: 01/30/05
Posts: 217
Loc: FL
Last seen: 16 years, 9 months
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Re: whats going on? (INCUBATION stage) [Re: SHA2]
#5348334 - 02/28/06 05:26 AM (17 years, 10 months ago) |
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patience. If it's infected you will know, if they aren't you will know. It took my first grow jars about 4-5 days to show signs of growth. Although I had a shitty incubator (jars in an empty 18 can budweiser case on top of my comp tower) give it time mang...
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Delinquentes
TIMMMAYYYYYYY


Registered: 02/07/06
Posts: 543
Loc: Hidin' in a bowl of rice
Last seen: 3 months, 16 days
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Re: whats going on? (INCUBATION stage) [Re: Woland]
#5348418 - 02/28/06 07:00 AM (17 years, 10 months ago) |
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I figure you just need to wait. It took me at least 4 days to get eny kind of growth that I could identify.
If you can't touch the jars, they are too hot to inoculate. Mine seem to cool really quickly though. I let them sit on a cold window sill.
In a week, you'll be back showing us pictures of the White Mycellium and we will all oooh and ahhh and all will be right in the world.
-------------------- "The price of meat has just gone up, and your old lady has just gone down!" ~~ Zappa Click Here for a ghetto ozone generator Tek. Want to learn more about ozone as a bacteria killer? Click Here
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SHA2
Stranger

Registered: 02/23/06
Posts: 23
Last seen: 17 years, 7 months
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Re: whats going on? (INCUBATION stage) [Re: Delinquentes]
#5350162 - 02/28/06 04:23 PM (17 years, 10 months ago) |
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yeah thanks for the comments.
No the jars were pretty cool when i injected the spores. just i could feel it was a lil bit warmer than my hands. so hopefully it should be ok.
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esdfsfd
Stranger

Registered: 12/17/05
Posts: 350
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Re: whats going on? (INCUBATION stage) [Re: SHA2]
#5350236 - 02/28/06 04:48 PM (17 years, 10 months ago) |
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put marbles or broken glass in the boiling pot next time you sterilize that way so your jars don't bust. Also, no way you overcooked your jars with steam...I think you should just wait and see.
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Hypercube
80 SRM



Registered: 12/18/05
Posts: 814
Last seen: 11 years, 22 days
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Re: whats going on? (INCUBATION stage) [Re: esdfsfd]
#5350248 - 02/28/06 04:53 PM (17 years, 10 months ago) |
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I assume your substrate is BRF/verm.
If so, be prepared to wait up to THREE WEEKS to see the first signs of germination. I've waited that long before, it sucks. Move up to grains.
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