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OfflineAkashaPrinciple
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Registered: 05/19/05
Posts: 289
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Weird thing happned while innoculating...
    #5347610 - 02/27/06 10:56 PM (17 years, 10 months ago)

Yesterday i steam steralised a BRF jar, i had plumbers tape over the 4 holes in the lid, but i loosened the outer ring which opens or closes the jar. When steaming was over (1.5 hrs) i left it to cool for about an hr and tightned the lid. I left it to cool over night and inoculated with a LC syringe, except i didnt remove the tape, i just pierced through it with the needle. As i did this on the first hole, i heard gas escaping (no smell), then i looked at my syringe and the plunger was moving down by itself and LC was going into the jar. I stopped it (affter about 3.5 cc's) and did the rest of the holes with no problem, anyone had this before??


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"My confidant, As full and bright as I am, this light is not my own,
A million light reflections pass over me, It's source is bright and endless"


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Offlinelardnar
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Registered: 06/15/05
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Re: Weird thing happned while innoculating... [Re: AkashaPrinciple]
    #5347625 - 02/27/06 11:00 PM (17 years, 10 months ago)

While the jar is cooling it will try to suck in air creating a vacuum. This must have sealed the rubber ring on the lid even though the ring was loose and when you stuck the needle in the jar the vacuum pulled the LC in

Nothing to worry about


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OfflineAkashaPrinciple
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Registered: 05/19/05
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Last seen: 10 years, 8 months
Re: Weird thing happned while innoculating... [Re: lardnar]
    #5347634 - 02/27/06 11:01 PM (17 years, 10 months ago)

ok cool, would it matter if upto 4cc's went into the hole?? Im hoping it wont put too much moisture in the jar.


--------------------
And in my darkest moment, fetal and weeping. The moon tells me a secret.
"My confidant, As full and bright as I am, this light is not my own,
A million light reflections pass over me, It's source is bright and endless"


TOOL - 'Reflection'




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OfflineHoss
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Registered: 06/13/05
Posts: 297
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Re: Weird thing happned while innoculating... [Re: AkashaPrinciple]
    #5347651 - 02/27/06 11:05 PM (17 years, 10 months ago)

Yes. Happens to every LC I make.

The inside of the jar had very very low pressure. When you stuck the needle in, the vacuum made by the low pressure in the jar pulled the contents of your syringe out on its own.

Every LC I make, I have to go in with an airport syringe and equalize the pressure, otherwise I'll lose an entire spore syringe to one LC. =[


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