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boomerman
psychobilly

Registered: 02/21/06
Posts: 294
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sterilization
#5344812 - 02/27/06 10:35 AM (17 years, 10 months ago) |
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I went out last night and bought a ton of shit, such as lysol, 409, anti bacterial soap and gerx hand sanitizer, dust masks, drinking water and some h202, and all the stuff to make a terrarium. I plan on steralizing the jars in a pressure cooker then inoculate the jars on the kitchen table. I am going to clean the whole house then dissenfect the kitchen with all the cleaners. my table is about 2 steps away from the stove, and if i follow the pf tek with tapes over the wholes in the jar, is it going to be safe for inoculation? is there anything else i should do before i inoculate for lesser chance of contamination - i am going to steralize the needle as well.
Should my terrarium be sterilized as well? And how should the perlite be rinsed of before putting it into the terrarium? and finaly, how much peroxide would i put into 1 gallon of water? I have seen the ratios for the formula but as you can probably tell from my spelling im not a math wizzard. Thanks
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coda
Banjo Goiter


Registered: 03/20/01
Posts: 8,750
Last seen: 10 months, 3 days
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Re: sterilization [Re: boomerman]
#5344862 - 02/27/06 10:52 AM (17 years, 10 months ago) |
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no need to sterlize the FC
Perlite doesnt need to be rinsed, just let it soak in a pot for 20-30 mins then strain it through a colander
1/4 cup of peroxide to 1 gal of water = 3%
Your sterile procedure is a bit overboard, but fine.
-------------------- To get really high is to forget yourself. And to forget yourself is to see everything else. And to see everything else is to become an understanding molecule in evolution, a conscious tool of the universe. And I think every human being should be a conscious tool of the universe. . . . -JG i really am glad you came back to us instead of taking the other path. *hug* -A_S (RIP your final words to me will never be forgotten)
 Don't fuck with the laughing jesus.
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: sterilization [Re: coda]
#5344876 - 02/27/06 10:57 AM (17 years, 10 months ago) |
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Quote:
coda said: Your sterile procedure is a bit overboard, but fine.
I concur. The main part you want to make sure you get right is sterilizing the jars. Also make sure to sterilize the needle of the inoculation syringe. Other than that, you can't really fuck up the PF tek unless the syringe was bad. I started out spraying Lysol and wiping everything down, but it's not really neccessary for the PF tek. The jar and dry vermiculite layer will keep out the nasties just fine.
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Quick WBS Prep
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g00n
Goombodias


Registered: 08/19/05
Posts: 77
Loc: Sothern Cali!!!
Last seen: 16 years, 6 months
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Re: sterilization [Re: boomerman]
#5344895 - 02/27/06 11:04 AM (17 years, 10 months ago) |
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With as much care as it seems you are taking, you should be just fine. If I were you I would make sure to pay great attention to keeping the air flow down when you are innoculating. If you are clean and make sure there isn't a wind passing through your kitchen you should do great.
-------------------- I run with the hatchet and claim the PSYCHOPATHIC!!!
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thenewguy05
The Mushroom Man


Registered: 02/11/05
Posts: 2,123
Loc: My Underground Layer
Last seen: 2 years, 5 months
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Re: sterilization [Re: g00n]
#5344902 - 02/27/06 11:08 AM (17 years, 10 months ago) |
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I always wipe down the fruiting chamber with alcohol before assembleing it. i never washed the perlite. and h2o2 i beleive is 13 ounces per gallon however a good ole fashion glug glug glug usually does the trick.
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