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Invisibleboomerman
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Registered: 02/21/06
Posts: 294
spores
    #5344743 - 02/27/06 10:15 AM (17 years, 10 months ago)

w hat would happen if i picked a matures shroom with sterile utensils, and immedeatly put it into a cake jar that has sterilized? DO you absoultly have to use a syringe to inoculate jars?


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Offlinejamman
SCI fan

Registered: 12/14/05
Posts: 280
Last seen: 16 years, 10 months
Re: spores [Re: boomerman]
    #5344763 - 02/27/06 10:23 AM (17 years, 10 months ago)

b4 this gets closed

a mature mushroom drops spores, which go into syringes.  The actual fruit body will not colonize a jar. You need to do a little research before you waste your resources.

Just eat the mushroom, thats the best advice I can give you

actually thats not true, you can use a live mushroom to make a culture.  It takes a small amount of work but its possible, i dont think it would work like he wants it to however.

The thing is boomerman you couldn't do that simply because the sterility issue would be too much.  You'd introduce too many variables into your incubation stage that shouldn't be there.  Just print the cap and make syinges out of it, there's a reason these things have been around forever :laugh:


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Edited by coda (02/27/06 10:55 AM)


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InvisibleFooMan
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Re: spores [Re: boomerman]
    #5344775 - 02/27/06 10:25 AM (17 years, 10 months ago)

So you are going to put a possibly contaminated mushroom cap into a sterilized jar and expect growth? Make a spore print, buy some agar plates and isolate some aggressive myc. Inoculate the jar in sterile conditions with that and you'll probably have much better results. If you don't know what the hell I'm talking about, which is likely by the question you posted, READ!


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Offlinecoda
Banjo Goiter
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Registered: 03/20/01
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Re: spores [Re: jamman]
    #5344875 - 02/27/06 10:57 AM (17 years, 10 months ago)

Quote:

jamman said:
b4 this gets closed

a mature mushroom drops spores, which go into syringes.  The actual fruit body will not colonize a jar. You need to do a little research before you waste your resources.

Just eat the mushroom, thats the best advice I can give you.




actually thats not true, you can use a live mushroom to make a culture.  It takes a small amount of work but its possible, i dont think it would work like he wants it to however.

The thing is boomerman you couldn't do that simply because the sterility issue would be too much.  You'd introduce too many variables into your incubation stage that shouldn't be there.  Just print the cap and make syinges out of it, there's a reason these things have been around forever :laugh:


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To get really high is to forget yourself. And to forget yourself is to see everything else. And to see everything else is to become an understanding molecule in evolution, a conscious tool of the universe. And I think every human being should be a conscious tool of the universe. . . .

-JG

i really am glad you came back to us instead of taking the other path. *hug*

-A_S (RIP your final words to me will never be forgotten)



Don't fuck with the laughing jesus.


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Offlinejamman
SCI fan

Registered: 12/14/05
Posts: 280
Last seen: 16 years, 10 months
Re: spores [Re: coda]
    #5344888 - 02/27/06 11:02 AM (17 years, 10 months ago)

What isn't true, that a fruit body will not colonize a cake jar?


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Offlinecoda
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Re: spores [Re: jamman]
    #5344893 - 02/27/06 11:03 AM (17 years, 10 months ago)

yup :smile:


--------------------
To get really high is to forget yourself. And to forget yourself is to see everything else. And to see everything else is to become an understanding molecule in evolution, a conscious tool of the universe. And I think every human being should be a conscious tool of the universe. . . .

-JG

i really am glad you came back to us instead of taking the other path. *hug*

-A_S (RIP your final words to me will never be forgotten)



Don't fuck with the laughing jesus.


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Offlinejamman
SCI fan

Registered: 12/14/05
Posts: 280
Last seen: 16 years, 10 months
Re: spores [Re: coda]
    #5344903 - 02/27/06 11:08 AM (17 years, 10 months ago)

not to steal this thread, but wouldn't it rot and produce contams?


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Offlinecoda
Banjo Goiter
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Re: spores [Re: jamman]
    #5344910 - 02/27/06 11:12 AM (17 years, 10 months ago)

if he just threw a mushroom in there and expected it to grow most likely yes.  If he followed correct cloning procedure then no. :smile:


--------------------
To get really high is to forget yourself. And to forget yourself is to see everything else. And to see everything else is to become an understanding molecule in evolution, a conscious tool of the universe. And I think every human being should be a conscious tool of the universe. . . .

-JG

i really am glad you came back to us instead of taking the other path. *hug*

-A_S (RIP your final words to me will never be forgotten)



Don't fuck with the laughing jesus.


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Offlinethenewguy05
The Mushroom Man
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Registered: 02/11/05
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Re: spores [Re: jamman]
    #5344913 - 02/27/06 11:13 AM (17 years, 10 months ago)

this method has very very low possibility. i have taken spore prints in the past that have started growing on the paper from the stalk where it was cut. but i don't beleive that your original plan was to grow from live tissue.


Edited by thenewguy05 (02/27/06 11:15 AM)


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Offlinejamman
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Registered: 12/14/05
Posts: 280
Last seen: 16 years, 10 months
Re: spores [Re: coda]
    #5344920 - 02/27/06 11:18 AM (17 years, 10 months ago)

Doesn't correct cloning procedure involve either agar or water with nutrients?


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Invisibleagar
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Re: spores [Re: jamman]
    #5344939 - 02/27/06 11:24 AM (17 years, 10 months ago)

You can clone, by placeing a small piece of mushroom tissue in an LC.
(takes very sterile procedures)

I use a biopsy needle gun to extract clean tissue from inside a stem, then inject into an prepared LC.


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Offlinecoda
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Re: spores [Re: agar]
    #5344942 - 02/27/06 11:26 AM (17 years, 10 months ago)

there's a bunch of ways to clone, one easy way would be to turn the mushroom into a slurry and inject that into either a grain jar or a LC.


--------------------
To get really high is to forget yourself. And to forget yourself is to see everything else. And to see everything else is to become an understanding molecule in evolution, a conscious tool of the universe. And I think every human being should be a conscious tool of the universe. . . .

-JG

i really am glad you came back to us instead of taking the other path. *hug*

-A_S (RIP your final words to me will never be forgotten)



Don't fuck with the laughing jesus.


Extras: Filter Print Post Top
Offlinejamman
SCI fan

Registered: 12/14/05
Posts: 280
Last seen: 16 years, 10 months
Re: spores [Re: coda]
    #5344955 - 02/27/06 11:33 AM (17 years, 10 months ago)

Oh, IC. I thought that the slurry could only be injected into LC or water, I didn't know you could knock up grain jars with it.


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InvisibleFooMan
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Re: spores [Re: agar]
    #5344959 - 02/27/06 11:36 AM (17 years, 10 months ago)

Quote:

agar said:
I use a biopsy needle gun to extract clean tissue from inside a stem, then inject into an prepared LC.





Where could I purchase one of these? It doesn't look like something you can get without some paperwork involved.


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Quick WBS Prep


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