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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Vermiculite

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Offlineandrewcubensis
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Registered: 01/14/02
Posts: 103
Last seen: 13 years, 7 days
TC HELP!
    #534192 - 01/28/02 06:30 PM (15 years, 28 days ago)

hey guys,
well i finally got meself a pressure cooker. i did the jar at 1hr 15mins at 11.6lb/s (that how high it goes) now i left the jars to cool for 12 hours and innoculated them with a spore syring of TC.Ive about 200ml of BRF on vermiculite.i used 1 cc in each hole. any tips so far?


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Invisibleangryshroom
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Registered: 12/18/01
Posts: 7,262
Re: TC HELP! [Re: andrewcubensis]
    #534206 - 01/28/02 06:51 PM (15 years, 28 days ago)

Sounds good for now.

Just keep em warm and dark, and check them every other day for contam. I usually wash my hands and arms and then douse them with a little rubbing alchol before i check em :smile:


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OfflineSeussA
Error: divide byzero

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Registered: 04/27/01
Posts: 23,480
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Re: TC HELP! [Re: andrewcubensis]
    #534280 - 01/28/02 08:36 PM (15 years, 28 days ago)

The less you mess with them until they are ready to birth, the less likely you are to add a new contaminate. Whey my friend first started growing, she would check the jars daily... if not more often. After a few runs, the novelty wears out and it is easier to leave them for a week or two at a time. My friend recommends leaving the jars for a day or two after sterilization, but before innoculation, to see if any nasties start to grow. She also makes one check after a week of growth. Any jars that show signs of contaminations should be removed and tossed in the trash. Remember, anything that touches a jar can carry contamination. When you play with them before they are ready (grin) be sure you first wash everything, especially your hands, down with 97% Isopropyl Alcohol (from the grocery store in with the bandaids and stuff... read the label, not all of them are 97%).


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Just another spore in the wind.


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Invisibleangryshroom
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Re: TC HELP! [Re: Seuss]
    #534303 - 01/28/02 08:59 PM (15 years, 28 days ago)

yeah best off not to check them too much ....

I just get so excited that I check em probably more than I should. I always get scared one iwll have contamination and I wont know about it and then when I check again 2 others will have it or something.. hehe


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Offlinefresh357
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Registered: 09/02/01
Posts: 493
Last seen: 14 years, 10 months
Re: TC HELP! [Re: angryshroom]
    #534352 - 01/28/02 09:43 PM (15 years, 28 days ago)

you can play with them all you want...as long as they are totally sealed off from the outside environment.
all that it might do is initiate invitro pinning.


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Anonymous

Re: TC HELP! [Re: andrewcubensis]
    #534405 - 01/28/02 10:34 PM (15 years, 28 days ago)

A couple notes....30 minutes will suffice in your cooker at that psi

It only takes about 4 hours to cool the jars off and I've never found waiting a couple days to be anything more than a waste of time.

I would use 1/2 cc per hole, that way you'll get a few more jars. Too much spore water can cause non-germination due to...too much water. But your 1 cc should still be ok, it just doesn't get you many jars.



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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Vermiculite

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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