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Offlinej21kickster
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Registered: 02/25/06
Posts: 69
Last seen: 17 years, 9 months
Substrain Development
    #5339824 - 02/25/06 11:57 PM (17 years, 11 months ago)

As generations of mushrooms progress from one parent to the next via spore collection, what is happening when people select more vigorous strains? I understand that there may be a slight effect on potentcy also. I guess im just trying to figure out how to sellect the best mushrooms/mycelium to use for the next batch. I would think that all of the spores from a fulsh would contain the same genetics, but since there is evidence of a change over generations, they must not be 100% identical, how is one to know what to look for and select for future propagation?


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OfflineRogerRabbitM
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Re: Substrain Development [Re: j21kickster]
    #5340508 - 02/26/06 10:15 AM (17 years, 10 months ago)

You don't.

Spores in this regard are like sperm and eggs. Look at a family with ten kids. All ten are different. Some are taller, some are smarter, some are meaner, etc. Each time you go back to spores, you have to select a substrain that has the qualities you want.
RR


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Offlinej21kickster
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Re: Substrain Development [Re: RogerRabbit]
    #5340525 - 02/26/06 10:21 AM (17 years, 10 months ago)

so is the only true way to preserve the strain, once you found one you like, through direct mycelium inoculation?


Edited by j21kickster (02/26/06 10:23 AM)


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OfflineRogerRabbitM
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Re: Substrain Development [Re: j21kickster]
    #5340565 - 02/26/06 10:37 AM (17 years, 10 months ago)

Correct. If you'll keep your master cultures in the refrigerator in test tubes, you can remove a very small piece once every few months to keep fresh material for growing. Once a year, remove a small piece from the test tube, and let it grow in a petri dish for three days or so, then remove a small piece from the petri dish and use it to inoculate a fresh test tube. If you'll do this, you can keep the same culture for twenty years or more without any problems from senesence.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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Offlinej21kickster
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Registered: 02/25/06
Posts: 69
Last seen: 17 years, 9 months
Re: Substrain Development [Re: RogerRabbit]
    #5340859 - 02/26/06 12:21 PM (17 years, 10 months ago)

thanks for the info


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